Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa
Abstract Roylea cinerea (D.Don) Baillon an indigenous medicinal plant of Lamiaceae family used for the treatment of several diseases. In the present study, its aqueous (leaves) extract was tested for genoprotective action against atrazine-induced chromosomal aberrations in the root tip cells of Alli...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16813-z |
| _version_ | 1818509834819993600 |
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| author | Farhana Rashid Davinder Singh Shivani Attri Prabhjot Kaur Harneetpal Kaur Pallvi Mohana Jahangeer Quadar Adarsh Pal Vig Astha Bhatia Balbir Singh Harpreet Walia Saroj Arora |
| author_facet | Farhana Rashid Davinder Singh Shivani Attri Prabhjot Kaur Harneetpal Kaur Pallvi Mohana Jahangeer Quadar Adarsh Pal Vig Astha Bhatia Balbir Singh Harpreet Walia Saroj Arora |
| author_sort | Farhana Rashid |
| collection | DOAJ |
| description | Abstract Roylea cinerea (D.Don) Baillon an indigenous medicinal plant of Lamiaceae family used for the treatment of several diseases. In the present study, its aqueous (leaves) extract was tested for genoprotective action against atrazine-induced chromosomal aberrations in the root tip cells of Allium cepa. Atrazine is a herbicide of triazine class commonly used to inhibit the growth of broad leaf and grassy weeds. In order to find the concentration of atrazine that exhibits maximum toxicity, its different concentrations (1, 5 and 10 µg/mL) were tested. It was observed that 10 µg/mL concentration was more toxic as it reduced the mitotic index and also increased the chromosomal aberrations. Among all the tested concentrations of aqueous (leaves) extracts (0.25. 0.5, 1.0, 1.5 and 3.0 µg/mL), the3.0 µg/mL concentration in both modes of experiments i.e. pre and post showed a significant reduction in chromosomal aberrations induced by atrazine. To understand the mechanism of protection by plant extract on atrazine-induced chromosomal abnormalities the RT-qPCR studies were conducted to observe the expression of marker genes Cyclin-dependent kinases (CDKs) (CDKA:1, CDKB2:1 and CDKD1:1. For this, the RNA was extracted from root tips treated with extract along with atrazine by TRIzol®. It was observed that aqueous extract of Roylea cinerea (D.Don) Baillon leaves upregulated the CDKs gene expression in both the modes i.e. pre and post treatments. A critical analysis of results indicated that aqueous extract ameliorated the chromosomal aberrations caused by atrazine which may be be due to the increased expression level of CDKs genes. |
| first_indexed | 2024-12-10T22:50:52Z |
| format | Article |
| id | doaj.art-e599f9261c234f699aac559034b16613 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T22:50:52Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e599f9261c234f699aac559034b166132022-12-22T01:30:26ZengNature PortfolioScientific Reports2045-23222022-07-0112111310.1038/s41598-022-16813-zModulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepaFarhana Rashid0Davinder Singh1Shivani Attri2Prabhjot Kaur3Harneetpal Kaur4Pallvi Mohana5Jahangeer Quadar6Adarsh Pal Vig7Astha Bhatia8Balbir Singh9Harpreet Walia10Saroj Arora11Department of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Pharmaceutical Sciences, Guru Nanak Dev UniversityDepartment of Botany and Environment Studies, DAV UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityAbstract Roylea cinerea (D.Don) Baillon an indigenous medicinal plant of Lamiaceae family used for the treatment of several diseases. In the present study, its aqueous (leaves) extract was tested for genoprotective action against atrazine-induced chromosomal aberrations in the root tip cells of Allium cepa. Atrazine is a herbicide of triazine class commonly used to inhibit the growth of broad leaf and grassy weeds. In order to find the concentration of atrazine that exhibits maximum toxicity, its different concentrations (1, 5 and 10 µg/mL) were tested. It was observed that 10 µg/mL concentration was more toxic as it reduced the mitotic index and also increased the chromosomal aberrations. Among all the tested concentrations of aqueous (leaves) extracts (0.25. 0.5, 1.0, 1.5 and 3.0 µg/mL), the3.0 µg/mL concentration in both modes of experiments i.e. pre and post showed a significant reduction in chromosomal aberrations induced by atrazine. To understand the mechanism of protection by plant extract on atrazine-induced chromosomal abnormalities the RT-qPCR studies were conducted to observe the expression of marker genes Cyclin-dependent kinases (CDKs) (CDKA:1, CDKB2:1 and CDKD1:1. For this, the RNA was extracted from root tips treated with extract along with atrazine by TRIzol®. It was observed that aqueous extract of Roylea cinerea (D.Don) Baillon leaves upregulated the CDKs gene expression in both the modes i.e. pre and post treatments. A critical analysis of results indicated that aqueous extract ameliorated the chromosomal aberrations caused by atrazine which may be be due to the increased expression level of CDKs genes.https://doi.org/10.1038/s41598-022-16813-z |
| spellingShingle | Farhana Rashid Davinder Singh Shivani Attri Prabhjot Kaur Harneetpal Kaur Pallvi Mohana Jahangeer Quadar Adarsh Pal Vig Astha Bhatia Balbir Singh Harpreet Walia Saroj Arora Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa Scientific Reports |
| title | Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa |
| title_full | Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa |
| title_fullStr | Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa |
| title_full_unstemmed | Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa |
| title_short | Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa |
| title_sort | modulation of atrazine induced chromosomal aberrations and cyclin dependent kinases by aqueous extract of roylea cinerea d don baillon leaves in allium cepa |
| url | https://doi.org/10.1038/s41598-022-16813-z |
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