Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children
IntroductionThe emergence of multidrug-resistant Pseudomonas aeruginosa poses a global threat, but the distribution and resistance profiling are unclear, especially in young children. Infections due to P. aeruginosa are common, associated with high mortality, and increasingly β-lactam drug resistant...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1168096/full |
| _version_ | 1797821461574975488 |
|---|---|
| author | Sandip Patil Sandip Patil Xiaowen Chen Shaowei Dong Huirong Mai Bruno Silvester Lopes Bruno Silvester Lopes Sixi Liu Feiqiu Wen Feiqiu Wen |
| author_facet | Sandip Patil Sandip Patil Xiaowen Chen Shaowei Dong Huirong Mai Bruno Silvester Lopes Bruno Silvester Lopes Sixi Liu Feiqiu Wen Feiqiu Wen |
| author_sort | Sandip Patil |
| collection | DOAJ |
| description | IntroductionThe emergence of multidrug-resistant Pseudomonas aeruginosa poses a global threat, but the distribution and resistance profiling are unclear, especially in young children. Infections due to P. aeruginosa are common, associated with high mortality, and increasingly β-lactam drug resistant.MethodsWe studied the molecular epidemiology and antibiotic resistance mechanisms in 294 clinicalisolates of P. aeruginosa from a pediatric hospital in China. Non-duplicate isolates were recovered from clinical cases and were identified using an API-20 kit followed by antimicrobial susceptibility testing using the VITEK®2 compact system (BioMerieux, France) and also by broth dilution method. In addition, a double-disc synergy test for the ESBL/E-test for MBL was performed. The presence of beta-lactamases, plasmid types, and sequence types was determined by PCR and sequencing.ResultsFifty-six percent (n = 164) of the isolates were resistant to piperacillin–tazobactam, followed by cefepime (40%; n = 117), ceftazidime (39%; n = 115), imipenem (36%; n = 106), meropenem (33%; n = 97), and ciprofloxacin (32%; n = 94). Forty-two percent (n = 126) of the isolates were positive for ESBL according to the double-disc synergy test. The blaCTX-M-15 cephalosporinase was observed in 32% (n = 40/126), while 26% (n = 33/126) werepositive for blaNDM-1 carbapenemase. Aminoglycoside resistance gene aac(3)IIIawas observed in 16% (n = 20/126), and glycylcyclines resistance gene tet(A) was observed in 12% (n = 15/126) of the isolates. A total of 23 sequence types were detected, including ST1963 (12%; n = 16), followed by ST381 (11%; n = 14), ST234 (10%; n = 13), ST145 (58%; n = 10), ST304 (57%; n = 9), ST663 (5%; n = 7), and a novel strain. In ESBL-producing P. aeruginosa, 12 different Incompatibility groups (Inc) were observed, the most common being IncFI, IncFIS, and IncA/C. The MOBP was the most common plasmid type, followed by MOBH, MOBF, and MOBQ.DiscussionOur data suggest that the spread of antibiotic resistance is likely due toclonal spread and dissemination of different clinical strains of P. aeruginosa harbouring different plasmids. This is a growing threat in hospitals particularly in young children which needs robust prevention strategies. |
| first_indexed | 2024-03-13T09:53:02Z |
| format | Article |
| id | doaj.art-e59af71ee5f04a919d1f3ae47665d58d |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-13T09:53:02Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e59af71ee5f04a919d1f3ae47665d58d2023-05-24T05:39:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-05-011310.3389/fcimb.2023.11680961168096Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young childrenSandip Patil0Sandip Patil1Xiaowen Chen2Shaowei Dong3Huirong Mai4Bruno Silvester Lopes5Bruno Silvester Lopes6Sixi Liu7Feiqiu Wen8Feiqiu Wen9Department of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaPaediatric Research Institute, Shenzhen Children’s Hospital, Shenzhen, ChinaPaediatric Research Institute, Shenzhen Children’s Hospital, Shenzhen, ChinaPaediatric Research Institute, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaSchool of Health and Life Sciences, Teesside University, Middlesbrough, United KingdomNational Horizons Centre, Teesside University, Darlington, United KingdomDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaPaediatric Research Institute, Shenzhen Children’s Hospital, Shenzhen, ChinaIntroductionThe emergence of multidrug-resistant Pseudomonas aeruginosa poses a global threat, but the distribution and resistance profiling are unclear, especially in young children. Infections due to P. aeruginosa are common, associated with high mortality, and increasingly β-lactam drug resistant.MethodsWe studied the molecular epidemiology and antibiotic resistance mechanisms in 294 clinicalisolates of P. aeruginosa from a pediatric hospital in China. Non-duplicate isolates were recovered from clinical cases and were identified using an API-20 kit followed by antimicrobial susceptibility testing using the VITEK®2 compact system (BioMerieux, France) and also by broth dilution method. In addition, a double-disc synergy test for the ESBL/E-test for MBL was performed. The presence of beta-lactamases, plasmid types, and sequence types was determined by PCR and sequencing.ResultsFifty-six percent (n = 164) of the isolates were resistant to piperacillin–tazobactam, followed by cefepime (40%; n = 117), ceftazidime (39%; n = 115), imipenem (36%; n = 106), meropenem (33%; n = 97), and ciprofloxacin (32%; n = 94). Forty-two percent (n = 126) of the isolates were positive for ESBL according to the double-disc synergy test. The blaCTX-M-15 cephalosporinase was observed in 32% (n = 40/126), while 26% (n = 33/126) werepositive for blaNDM-1 carbapenemase. Aminoglycoside resistance gene aac(3)IIIawas observed in 16% (n = 20/126), and glycylcyclines resistance gene tet(A) was observed in 12% (n = 15/126) of the isolates. A total of 23 sequence types were detected, including ST1963 (12%; n = 16), followed by ST381 (11%; n = 14), ST234 (10%; n = 13), ST145 (58%; n = 10), ST304 (57%; n = 9), ST663 (5%; n = 7), and a novel strain. In ESBL-producing P. aeruginosa, 12 different Incompatibility groups (Inc) were observed, the most common being IncFI, IncFIS, and IncA/C. The MOBP was the most common plasmid type, followed by MOBH, MOBF, and MOBQ.DiscussionOur data suggest that the spread of antibiotic resistance is likely due toclonal spread and dissemination of different clinical strains of P. aeruginosa harbouring different plasmids. This is a growing threat in hospitals particularly in young children which needs robust prevention strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1168096/fullP. aeruginosaESBLsantimicrobial susceptibilitymlstPBRTMOB typing |
| spellingShingle | Sandip Patil Sandip Patil Xiaowen Chen Shaowei Dong Huirong Mai Bruno Silvester Lopes Bruno Silvester Lopes Sixi Liu Feiqiu Wen Feiqiu Wen Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children Frontiers in Cellular and Infection Microbiology P. aeruginosa ESBLs antimicrobial susceptibility mlst PBRT MOB typing |
| title | Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children |
| title_full | Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children |
| title_fullStr | Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children |
| title_full_unstemmed | Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children |
| title_short | Resistance genomics and molecular epidemiology of high-risk clones of ESBL-producing Pseudomonas aeruginosa in young children |
| title_sort | resistance genomics and molecular epidemiology of high risk clones of esbl producing pseudomonas aeruginosa in young children |
| topic | P. aeruginosa ESBLs antimicrobial susceptibility mlst PBRT MOB typing |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1168096/full |
| work_keys_str_mv | AT sandippatil resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT sandippatil resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT xiaowenchen resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT shaoweidong resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT huirongmai resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT brunosilvesterlopes resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT brunosilvesterlopes resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT sixiliu resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT feiqiuwen resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren AT feiqiuwen resistancegenomicsandmolecularepidemiologyofhighriskclonesofesblproducingpseudomonasaeruginosainyoungchildren |