Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells
As emerging contaminants, nano-plastics have become a major cause for concern for their adverse effects on the ecosystem and human health. The nano-sized properties of nano-plastics enable their exposure risks to humans through the food chain or other ways. However, the fate and adverse impact of na...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/5/215 |
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| author | Yiqi Fu Mengqi Fan Liwang Xu Hui Wang Qinglian Hu Yuanxiang Jin |
| author_facet | Yiqi Fu Mengqi Fan Liwang Xu Hui Wang Qinglian Hu Yuanxiang Jin |
| author_sort | Yiqi Fu |
| collection | DOAJ |
| description | As emerging contaminants, nano-plastics have become a major cause for concern for their adverse effects on the ecosystem and human health. The nano-sized properties of nano-plastics enable their exposure risks to humans through the food chain or other ways. However, the fate and adverse impact of nano-plastics on the human cardiovascular system are lacking. In this regard, the human umbilical vein endothelial cell line HUVEC was applied as a cell model to investigate the biological effects of noncharged polystyrene nano-plastics (PS NPs) and amino-functionalized nano-plastics (NH<sub>2</sub>-PS NPs). The positively charged PS NPs exhibited higher cytotoxicity to HUVEC, as evidenced by the decreased cell viability, enhanced ROS generation, and decreased mitochondria membrane potential triggered by NH<sub>2</sub>-PS NPs. Importantly, RT-PCR analysis revealed that NH<sub>2</sub>-PS NPs dysregulated the mitochondrial dynamics, replication, and function-related gene expression. This study demonstrated that NH<sub>2</sub>-PS NPs presented higher risks to endothelial cells than non-charged nano-plastics by interfering with mitochondria, which supported the direct evidence and expanded the potential risks of PS NPs. |
| first_indexed | 2024-03-10T01:42:45Z |
| format | Article |
| id | doaj.art-e59bdd1d00a54c2eb9f7427db840300a |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T01:42:45Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-e59bdd1d00a54c2eb9f7427db840300a2023-11-23T13:21:18ZengMDPI AGToxics2305-63042022-04-0110521510.3390/toxics10050215Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial CellsYiqi Fu0Mengqi Fan1Liwang Xu2Hui Wang3Qinglian Hu4Yuanxiang Jin5College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, ChinaAs emerging contaminants, nano-plastics have become a major cause for concern for their adverse effects on the ecosystem and human health. The nano-sized properties of nano-plastics enable their exposure risks to humans through the food chain or other ways. However, the fate and adverse impact of nano-plastics on the human cardiovascular system are lacking. In this regard, the human umbilical vein endothelial cell line HUVEC was applied as a cell model to investigate the biological effects of noncharged polystyrene nano-plastics (PS NPs) and amino-functionalized nano-plastics (NH<sub>2</sub>-PS NPs). The positively charged PS NPs exhibited higher cytotoxicity to HUVEC, as evidenced by the decreased cell viability, enhanced ROS generation, and decreased mitochondria membrane potential triggered by NH<sub>2</sub>-PS NPs. Importantly, RT-PCR analysis revealed that NH<sub>2</sub>-PS NPs dysregulated the mitochondrial dynamics, replication, and function-related gene expression. This study demonstrated that NH<sub>2</sub>-PS NPs presented higher risks to endothelial cells than non-charged nano-plastics by interfering with mitochondria, which supported the direct evidence and expanded the potential risks of PS NPs.https://www.mdpi.com/2305-6304/10/5/215polystyrene nanoparticlesHUVECoxidative stressmitochondria |
| spellingShingle | Yiqi Fu Mengqi Fan Liwang Xu Hui Wang Qinglian Hu Yuanxiang Jin Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells Toxics polystyrene nanoparticles HUVEC oxidative stress mitochondria |
| title | Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells |
| title_full | Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells |
| title_fullStr | Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells |
| title_full_unstemmed | Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells |
| title_short | Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells |
| title_sort | amino functionalized polystyrene nano plastics induce mitochondria damage in human umbilical vein endothelial cells |
| topic | polystyrene nanoparticles HUVEC oxidative stress mitochondria |
| url | https://www.mdpi.com/2305-6304/10/5/215 |
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