Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows
ABSTRACT: We evaluated the effects of supplementing bacterial direct-fed microbial (DFM) on performance, apparent total-tract digestibility, rumen fermentation, and immune parameters of lactating dairy cows. One hundred fourteen multiparous Holstein cows (41 ± 7 DIM) were used in a randomized comple...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223004563 |
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| author | A.O. Oyebade S. Lee H. Sultana K. Arriola E. Duvalsaint C. Nino De Guzman I. Fernandez Marenchino L. Marroquin Pacheco F. Amaro L. Ghedin Ghizzi L. Mu H. Guan K.V. Almeida B. Rajo Andrade J. Zhao P. Tian C. Cheng Y. Jiang J. Driver O. Queiroz L.F. Ferraretto I.M. Ogunade A.T. Adesogan D. Vyas |
| author_facet | A.O. Oyebade S. Lee H. Sultana K. Arriola E. Duvalsaint C. Nino De Guzman I. Fernandez Marenchino L. Marroquin Pacheco F. Amaro L. Ghedin Ghizzi L. Mu H. Guan K.V. Almeida B. Rajo Andrade J. Zhao P. Tian C. Cheng Y. Jiang J. Driver O. Queiroz L.F. Ferraretto I.M. Ogunade A.T. Adesogan D. Vyas |
| author_sort | A.O. Oyebade |
| collection | DOAJ |
| description | ABSTRACT: We evaluated the effects of supplementing bacterial direct-fed microbial (DFM) on performance, apparent total-tract digestibility, rumen fermentation, and immune parameters of lactating dairy cows. One hundred fourteen multiparous Holstein cows (41 ± 7 DIM) were used in a randomized complete block design with an experiment comprising 14 d of a covariate (pre-experimental sample and data collection) and 91 d of an experimental period. Cows were blocked based on energy-corrected milk (ECM) yield during the covariate period and the following treatments were randomly assigned within each block: (1) control (CON), corn silage-based total mixed ration without DFM; (2) PRO-A, basal diet top-dressed with a mixture of Lactobacillus animalis and Propionibacterium freudenreichii at 3 × 109 cfu/d; and 3) PRO-B, basal diet top-dressed with a mixture of L. animalis, P. freudenreichii, Bacillus subtilis, and Bacillus licheniformis at 11.8 × 109 cfu/d. Milk yield, dry matter intake (DMI), and body weight were measured daily, while milk samples for component analysis were taken on 2 consecutive days of each week of data collection. Feces, urine, rumen, and blood samples were taken during the covariate period, wk 4, 7, 10, and 13 for estimation of digestibility, N-partitioning, rumen fermentation, plasma nutrient status and immune parameters. Treatments had no effect on DMI and milk yield. Fat-corrected milk (3.5% FCM) and milk fat yield were improved with PRO-B, while milk fat percent and feed efficiency (ECM/DMI) tended to increase with PRO-B compared with PRO-A and CON. Crude fat digestibility was greater with PRO-B compared with CON. Feeding CON and PRO-A resulted in higher total volatile fatty acid concentration relative to PRO-B. Percentage of neutrophils tended to be reduced with PRO-A compared with CON and PRO-B. The mean fluorescence intensity (MFI) of anti-CD44 antibody on granulocytes tended to be higher in PRO-B compared with CON. The MFI of anti-CD62L antibody on CD8+ T cells was lower in PRO-A than PRO-B, with PRO-A also showing a tendency to be lower than CON. This study indicates the potential of DFM to improve fat digestibility with consequential improvement in fat corrected milk yield, feed efficiency and milk fat yield by lactating dairy cows. The study findings also indicate that dietary supplementation with DFM may augment immune parameters or activation of immune cells, including granulocytes and T cells; however, the overall effects on immune parameters are inconclusive. |
| first_indexed | 2024-03-08T23:13:55Z |
| format | Article |
| id | doaj.art-e59d18807d3341f1b585f2cf04f894d7 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-08T23:13:55Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-e59d18807d3341f1b585f2cf04f894d72023-12-15T07:21:51ZengElsevierJournal of Dairy Science0022-03022023-12-011061286118626Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cowsA.O. Oyebade0S. Lee1H. Sultana2K. Arriola3E. Duvalsaint4C. Nino De Guzman5I. Fernandez Marenchino6L. Marroquin Pacheco7F. Amaro8L. Ghedin Ghizzi9L. Mu10H. Guan11K.V. Almeida12B. Rajo Andrade13J. Zhao14P. Tian15C. Cheng16Y. Jiang17J. Driver18O. Queiroz19L.F. Ferraretto20I.M. Ogunade21A.T. Adesogan22D. Vyas23Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, KY 40601College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, KY 40601MU Bond Life Sciences Center, University of Missouri–Columbia, Columbia, MO 65211Chr. Hansen A/S, Animal Health and Nutrition, B⊘ge Allé 10-12, DK-2970 H⊘rsholm, DenmarkDepartment of Dairy Science, University of Wisconsin–Madison, Madison, WI 53706Division of Animal and Nutritional Science, West Virginia University, Morgantown, WV 26506Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611; Corresponding authorABSTRACT: We evaluated the effects of supplementing bacterial direct-fed microbial (DFM) on performance, apparent total-tract digestibility, rumen fermentation, and immune parameters of lactating dairy cows. One hundred fourteen multiparous Holstein cows (41 ± 7 DIM) were used in a randomized complete block design with an experiment comprising 14 d of a covariate (pre-experimental sample and data collection) and 91 d of an experimental period. Cows were blocked based on energy-corrected milk (ECM) yield during the covariate period and the following treatments were randomly assigned within each block: (1) control (CON), corn silage-based total mixed ration without DFM; (2) PRO-A, basal diet top-dressed with a mixture of Lactobacillus animalis and Propionibacterium freudenreichii at 3 × 109 cfu/d; and 3) PRO-B, basal diet top-dressed with a mixture of L. animalis, P. freudenreichii, Bacillus subtilis, and Bacillus licheniformis at 11.8 × 109 cfu/d. Milk yield, dry matter intake (DMI), and body weight were measured daily, while milk samples for component analysis were taken on 2 consecutive days of each week of data collection. Feces, urine, rumen, and blood samples were taken during the covariate period, wk 4, 7, 10, and 13 for estimation of digestibility, N-partitioning, rumen fermentation, plasma nutrient status and immune parameters. Treatments had no effect on DMI and milk yield. Fat-corrected milk (3.5% FCM) and milk fat yield were improved with PRO-B, while milk fat percent and feed efficiency (ECM/DMI) tended to increase with PRO-B compared with PRO-A and CON. Crude fat digestibility was greater with PRO-B compared with CON. Feeding CON and PRO-A resulted in higher total volatile fatty acid concentration relative to PRO-B. Percentage of neutrophils tended to be reduced with PRO-A compared with CON and PRO-B. The mean fluorescence intensity (MFI) of anti-CD44 antibody on granulocytes tended to be higher in PRO-B compared with CON. The MFI of anti-CD62L antibody on CD8+ T cells was lower in PRO-A than PRO-B, with PRO-A also showing a tendency to be lower than CON. This study indicates the potential of DFM to improve fat digestibility with consequential improvement in fat corrected milk yield, feed efficiency and milk fat yield by lactating dairy cows. The study findings also indicate that dietary supplementation with DFM may augment immune parameters or activation of immune cells, including granulocytes and T cells; however, the overall effects on immune parameters are inconclusive.http://www.sciencedirect.com/science/article/pii/S0022030223004563milk fatfeed efficiencyprobioticsimmune functionmilk production |
| spellingShingle | A.O. Oyebade S. Lee H. Sultana K. Arriola E. Duvalsaint C. Nino De Guzman I. Fernandez Marenchino L. Marroquin Pacheco F. Amaro L. Ghedin Ghizzi L. Mu H. Guan K.V. Almeida B. Rajo Andrade J. Zhao P. Tian C. Cheng Y. Jiang J. Driver O. Queiroz L.F. Ferraretto I.M. Ogunade A.T. Adesogan D. Vyas Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows Journal of Dairy Science milk fat feed efficiency probiotics immune function milk production |
| title | Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| title_full | Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| title_fullStr | Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| title_full_unstemmed | Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| title_short | Effects of direct-fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| title_sort | effects of direct fed microbial supplementation on performance and immune parameters of lactating dairy cows |
| topic | milk fat feed efficiency probiotics immune function milk production |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223004563 |
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