The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis
Our recent study observed the combined beneficial effects of postbiotic butyrate on active vitamin D3-orchestrated innate immunity to <i>Salmonella</i> Colitis. There is increasing interest in the role of acyl hydrocarbon receptor (AhR) on colitis and innate immunity. Therefore, we inves...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/1/195 |
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| author | Fu-Chen Huang Shun-Chen Huang |
| author_facet | Fu-Chen Huang Shun-Chen Huang |
| author_sort | Fu-Chen Huang |
| collection | DOAJ |
| description | Our recent study observed the combined beneficial effects of postbiotic butyrate on active vitamin D3-orchestrated innate immunity to <i>Salmonella</i> Colitis. There is increasing interest in the role of acyl hydrocarbon receptor (AhR) on colitis and innate immunity. Therefore, we investigated the involvement of AhR in the effects. <i>Salmonella</i> colitis model is conducted with 6–8 w/o male C57BL/6 mice: Streptomycin (20 mg/mouse p.o.)-pretreated C57BL/6 mice were mock infected with sterile PBS or infected orally with 1 × 10<sup>8</sup> CFU of an <i>S. typhimurium</i> wild-type strain SL1344 for 48 h. Before and after the colitis induction, mice were oral gavage with active vitamin D3 0.2 μg/25 g mice (VD3) and/or postbiotics propionate (PP), in the absence of the presence of intraperitoneal injection of AhR inhibitor for 4 and 7 days, respectively. We observed AhR inhibitor counteracted the synergistic effects of PP and VD3 on reducing the severity of <i>Salmonella</i> colitis and body weight loss in C57BL/6 mice, reducing the cecal inflammatory but enhancing antimicrobial peptide mRNAs expression, and reducing the bacterial translocation in liver/spleen, compared to single treatment. It suggests the involvement of AhR on the synergistic effects of postbiotics PP and VD3 on the antibacterial and anti-inflammatory responses in <i>Salmonella</i> colitis and the potential biological treatment of <i>Salmonella</i> colitis. |
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| language | English |
| last_indexed | 2024-03-09T13:28:43Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-e59efa55b25c4e998e81d6c319e0431b2023-11-30T21:20:50ZengMDPI AGBiomedicines2227-90592023-01-0111119510.3390/biomedicines11010195The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> ColitisFu-Chen Huang0Shun-Chen Huang1Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanDepartment of Anatomic Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanOur recent study observed the combined beneficial effects of postbiotic butyrate on active vitamin D3-orchestrated innate immunity to <i>Salmonella</i> Colitis. There is increasing interest in the role of acyl hydrocarbon receptor (AhR) on colitis and innate immunity. Therefore, we investigated the involvement of AhR in the effects. <i>Salmonella</i> colitis model is conducted with 6–8 w/o male C57BL/6 mice: Streptomycin (20 mg/mouse p.o.)-pretreated C57BL/6 mice were mock infected with sterile PBS or infected orally with 1 × 10<sup>8</sup> CFU of an <i>S. typhimurium</i> wild-type strain SL1344 for 48 h. Before and after the colitis induction, mice were oral gavage with active vitamin D3 0.2 μg/25 g mice (VD3) and/or postbiotics propionate (PP), in the absence of the presence of intraperitoneal injection of AhR inhibitor for 4 and 7 days, respectively. We observed AhR inhibitor counteracted the synergistic effects of PP and VD3 on reducing the severity of <i>Salmonella</i> colitis and body weight loss in C57BL/6 mice, reducing the cecal inflammatory but enhancing antimicrobial peptide mRNAs expression, and reducing the bacterial translocation in liver/spleen, compared to single treatment. It suggests the involvement of AhR on the synergistic effects of postbiotics PP and VD3 on the antibacterial and anti-inflammatory responses in <i>Salmonella</i> colitis and the potential biological treatment of <i>Salmonella</i> colitis.https://www.mdpi.com/2227-9059/11/1/195acyl hydrocarbon receptorpostbioticsactive vitamin D3innate immunity<i>Salmonella</i> colitis |
| spellingShingle | Fu-Chen Huang Shun-Chen Huang The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis Biomedicines acyl hydrocarbon receptor postbiotics active vitamin D3 innate immunity <i>Salmonella</i> colitis |
| title | The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis |
| title_full | The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis |
| title_fullStr | The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis |
| title_full_unstemmed | The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis |
| title_short | The Critical Role of Acyl Hydrocarbon Receptor on the Combined Benefits of Postbiotic Propionate on Active Vitamin D3-Orchestrated Innate Immunity in <i>Salmonella</i> Colitis |
| title_sort | critical role of acyl hydrocarbon receptor on the combined benefits of postbiotic propionate on active vitamin d3 orchestrated innate immunity in i salmonella i colitis |
| topic | acyl hydrocarbon receptor postbiotics active vitamin D3 innate immunity <i>Salmonella</i> colitis |
| url | https://www.mdpi.com/2227-9059/11/1/195 |
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