Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys
Zearalenone (ZEN) is a mycotoxin produced by various Fusarium strains, that is present in food and feed raw materials worldwide, causing toxicity effects in animals and humans. This research aimed to explore the toxicokinetics of ZEN on female Dezhou donkeys following a single oral exposure dosage o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/16/1/51 |
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| author | Honglei Qu Yunduo Zheng Ruifen Kang Yulong Feng Pengshuai Li Yantao Wang Jie Cheng Cheng Ji Wenqiong Chai Qiugang Ma |
| author_facet | Honglei Qu Yunduo Zheng Ruifen Kang Yulong Feng Pengshuai Li Yantao Wang Jie Cheng Cheng Ji Wenqiong Chai Qiugang Ma |
| author_sort | Honglei Qu |
| collection | DOAJ |
| description | Zearalenone (ZEN) is a mycotoxin produced by various Fusarium strains, that is present in food and feed raw materials worldwide, causing toxicity effects in animals and humans. This research aimed to explore the toxicokinetics of ZEN on female Dezhou donkeys following a single oral exposure dosage of 2 mg/kg BW (body weight). The sample collection of donkeys plasma was carried out at 0, 5, 10, 15, 20, 30, 45, 60, 90 min, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h, 6 h, 9 h, 12 h, 24 h, 48 h, 72 h, 96 h and 120 h via intravenous catheter, and fecal and urinary samples were severally collected at 0 h and every 6 h until 120 h. The concentrations of ZEN, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), zearalanone (ZAN) in plasma, urine, and feces were detected by UPLC-MS/MS. Only ZEN was detected in plasma, and the maximum was 15.34 ± 5.12 µg/L occurred at 0.48 h after gavage. The total plasma clearance (Cl) of ZEN was 95.20 ± 8.01 L·kg·BW<sup>−1</sup>·h<sup>−1</sup>. In addition, the volume of distribution (Vd) was up to 216.17 ± 58.71 L/kg. The percentage of total ZEN (ZEN plus the main metabolites) excretion in feces and urine was 2.49% and 2.10%, respectively. In summary, ZEN was fast absorbed and relatively slowly excreted in female donkeys during 120 h after a single gavage, indicating a trend of wider tissue distribution and longer tissue persistence. |
| first_indexed | 2024-03-08T10:33:53Z |
| format | Article |
| id | doaj.art-e59fae779d0f475a821069117e7795a0 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-08T10:33:53Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-e59fae779d0f475a821069117e7795a02024-01-26T18:42:33ZengMDPI AGToxins2072-66512024-01-011615110.3390/toxins16010051Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou DonkeysHonglei Qu0Yunduo Zheng1Ruifen Kang2Yulong Feng3Pengshuai Li4Yantao Wang5Jie Cheng6Cheng Ji7Wenqiong Chai8Qiugang Ma9State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng 252201, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng 252201, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng 252201, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaLiaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252000, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaZearalenone (ZEN) is a mycotoxin produced by various Fusarium strains, that is present in food and feed raw materials worldwide, causing toxicity effects in animals and humans. This research aimed to explore the toxicokinetics of ZEN on female Dezhou donkeys following a single oral exposure dosage of 2 mg/kg BW (body weight). The sample collection of donkeys plasma was carried out at 0, 5, 10, 15, 20, 30, 45, 60, 90 min, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h, 6 h, 9 h, 12 h, 24 h, 48 h, 72 h, 96 h and 120 h via intravenous catheter, and fecal and urinary samples were severally collected at 0 h and every 6 h until 120 h. The concentrations of ZEN, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), zearalanone (ZAN) in plasma, urine, and feces were detected by UPLC-MS/MS. Only ZEN was detected in plasma, and the maximum was 15.34 ± 5.12 µg/L occurred at 0.48 h after gavage. The total plasma clearance (Cl) of ZEN was 95.20 ± 8.01 L·kg·BW<sup>−1</sup>·h<sup>−1</sup>. In addition, the volume of distribution (Vd) was up to 216.17 ± 58.71 L/kg. The percentage of total ZEN (ZEN plus the main metabolites) excretion in feces and urine was 2.49% and 2.10%, respectively. In summary, ZEN was fast absorbed and relatively slowly excreted in female donkeys during 120 h after a single gavage, indicating a trend of wider tissue distribution and longer tissue persistence.https://www.mdpi.com/2072-6651/16/1/51toxicokineticdonkeymycotoxinszearalenone |
| spellingShingle | Honglei Qu Yunduo Zheng Ruifen Kang Yulong Feng Pengshuai Li Yantao Wang Jie Cheng Cheng Ji Wenqiong Chai Qiugang Ma Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys Toxins toxicokinetic donkey mycotoxins zearalenone |
| title | Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys |
| title_full | Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys |
| title_fullStr | Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys |
| title_full_unstemmed | Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys |
| title_short | Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys |
| title_sort | toxicokinetics of zearalenone following oral administration in female dezhou donkeys |
| topic | toxicokinetic donkey mycotoxins zearalenone |
| url | https://www.mdpi.com/2072-6651/16/1/51 |
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