Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells
Despite significant advances in the understanding, prevention, and treatment of cancer, the disease continues to affect millions of people worldwide. Chemoradiation therapy is a rational approach that has already proven beneficial for several malignancies. However, the existence of toxicity to norma...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/17/3154 |
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| author | Olesya S. Malyarenko Timofey V. Malyarenko Alla A. Kicha Natalia V. Ivanchina Svetlana P. Ermakova |
| author_facet | Olesya S. Malyarenko Timofey V. Malyarenko Alla A. Kicha Natalia V. Ivanchina Svetlana P. Ermakova |
| author_sort | Olesya S. Malyarenko |
| collection | DOAJ |
| description | Despite significant advances in the understanding, prevention, and treatment of cancer, the disease continues to affect millions of people worldwide. Chemoradiation therapy is a rational approach that has already proven beneficial for several malignancies. However, the existence of toxicity to normal tissue is a serious limitation of this treatment modality. The aim of the present study is to investigate the ability of polar steroids from starfish <i>Patiria (=Asterina) pectinifera</i> to enhance the efficacy of radiation therapy in colorectal carcinoma cells. The cytotoxic activity of polar steroids and X-ray radiation against DLD-1, HCT 116, and HT-29 cells was determined by an MTS assay. The effect of compounds, X-ray, and their combination on colony formation was studied using the soft agar method. The molecular mechanism of the radiosensitizing activity of asterosaponin P<sub>1</sub> was elucidated by western blotting and the DNA comet assay. Polar steroids inhibited colony formation in the tested cells, and to a greater extent in HT-29 cells. Asterosaponin P<sub>1</sub> enhanced the efficacy of radiation and, as a result, reduced the number and size of the colonies of colorectal cancer cells. The radiosensitizing activity of asterosaponin P<sub>1</sub> was realized by apoptosis induction through the regulation of anti- and pro-apoptotic protein expression followed by caspase activation and DNA degradation. |
| first_indexed | 2024-04-13T12:44:11Z |
| format | Article |
| id | doaj.art-e5a0dc0f99984a6da2a5ed42eaf3d44b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T12:44:11Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e5a0dc0f99984a6da2a5ed42eaf3d44b2022-12-22T02:46:26ZengMDPI AGMolecules1420-30492019-08-012417315410.3390/molecules24173154molecules24173154Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma CellsOlesya S. Malyarenko0Timofey V. Malyarenko1Alla A. Kicha2Natalia V. Ivanchina3Svetlana P. Ermakova4G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 100-let Vladivostok Ave., 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 100-let Vladivostok Ave., 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 100-let Vladivostok Ave., 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 100-let Vladivostok Ave., 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 100-let Vladivostok Ave., 690022 Vladivostok, RussiaDespite significant advances in the understanding, prevention, and treatment of cancer, the disease continues to affect millions of people worldwide. Chemoradiation therapy is a rational approach that has already proven beneficial for several malignancies. However, the existence of toxicity to normal tissue is a serious limitation of this treatment modality. The aim of the present study is to investigate the ability of polar steroids from starfish <i>Patiria (=Asterina) pectinifera</i> to enhance the efficacy of radiation therapy in colorectal carcinoma cells. The cytotoxic activity of polar steroids and X-ray radiation against DLD-1, HCT 116, and HT-29 cells was determined by an MTS assay. The effect of compounds, X-ray, and their combination on colony formation was studied using the soft agar method. The molecular mechanism of the radiosensitizing activity of asterosaponin P<sub>1</sub> was elucidated by western blotting and the DNA comet assay. Polar steroids inhibited colony formation in the tested cells, and to a greater extent in HT-29 cells. Asterosaponin P<sub>1</sub> enhanced the efficacy of radiation and, as a result, reduced the number and size of the colonies of colorectal cancer cells. The radiosensitizing activity of asterosaponin P<sub>1</sub> was realized by apoptosis induction through the regulation of anti- and pro-apoptotic protein expression followed by caspase activation and DNA degradation.https://www.mdpi.com/1420-3049/24/17/3154starfish<i>Patiria (=Asterina) pectinifera</i>polar steroidscolorectal carcinoma cellsX-raycolony formationapoptosisDNA fragmentation |
| spellingShingle | Olesya S. Malyarenko Timofey V. Malyarenko Alla A. Kicha Natalia V. Ivanchina Svetlana P. Ermakova Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells Molecules starfish <i>Patiria (=Asterina) pectinifera</i> polar steroids colorectal carcinoma cells X-ray colony formation apoptosis DNA fragmentation |
| title | Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells |
| title_full | Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells |
| title_fullStr | Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells |
| title_full_unstemmed | Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells |
| title_short | Effects of Polar Steroids from the Starfish <i>Patiria (=Asterina) pectinifera</i> in Combination with X-Ray Radiation on Colony Formation and Apoptosis Induction of Human Colorectal Carcinoma Cells |
| title_sort | effects of polar steroids from the starfish i patiria asterina pectinifera i in combination with x ray radiation on colony formation and apoptosis induction of human colorectal carcinoma cells |
| topic | starfish <i>Patiria (=Asterina) pectinifera</i> polar steroids colorectal carcinoma cells X-ray colony formation apoptosis DNA fragmentation |
| url | https://www.mdpi.com/1420-3049/24/17/3154 |
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