CRISPR/Cas9-Mediated Generation of Mutant Lines in <i>Medicago truncatula</i> Indicates a Symbiotic Role of <i>MtLYK10</i> during Nodule Formation

CRISPR/Cas9-Mediated Generation of Mutant Lines in <i>Medicago truncatula</i> Indicates a Symbiotic Role of <i>MtLYK10</i> during Nodule Formation

CRISPR/Cas9 systems are commonly used for plant genome editing; however, the generation of homozygous mutant lines in <i>Medicago truncatula</i> remains challenging. Here, we present a CRISPR/Cas9-based protocol that allows the efficient generation of <i>M. truncatula</i> mut...

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Bibliographic Details
Main Authors: Chun-Xiao Zhang, Ru-Jie Li, Laura Baude, Didier Reinhardt, Zhi-Ping Xie, Christian Staehelin
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Biology
Subjects:
genome editing
LysM receptor kinase
plant tissue culture
symbiosis
Online Access:https://www.mdpi.com/2079-7737/13/1/53

Internet

https://www.mdpi.com/2079-7737/13/1/53

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