Assessment of Bacterial Contamination of Orthodontic Arch wire
Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manu...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
College of Dentistry/ University of Baghdad
2019-03-01
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Series: | Journal of Baghdad College of Dentistry |
Online Access: | https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/2578 |
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author | Suha S Hassan Nidhal H. Ghaib Batool H Al-Ghurabi |
author_facet | Suha S Hassan Nidhal H. Ghaib Batool H Al-Ghurabi |
author_sort | Suha S Hassan |
collection | DOAJ |
description | Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination. |
first_indexed | 2024-04-11T14:43:18Z |
format | Article |
id | doaj.art-e5ac72fae1d74a37900df1489be58842 |
institution | Directory Open Access Journal |
issn | 2311-5270 |
language | English |
last_indexed | 2024-04-11T14:43:18Z |
publishDate | 2019-03-01 |
publisher | College of Dentistry/ University of Baghdad |
record_format | Article |
series | Journal of Baghdad College of Dentistry |
spelling | doaj.art-e5ac72fae1d74a37900df1489be588422022-12-22T04:17:48ZengCollege of Dentistry/ University of BaghdadJournal of Baghdad College of Dentistry2311-52702019-03-0131110.26477/jbcd.v31i1.2578Assessment of Bacterial Contamination of Orthodontic Arch wireSuha S HassanNidhal H. GhaibBatool H Al-GhurabiBackground: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/2578 |
spellingShingle | Suha S Hassan Nidhal H. Ghaib Batool H Al-Ghurabi Assessment of Bacterial Contamination of Orthodontic Arch wire Journal of Baghdad College of Dentistry |
title | Assessment of Bacterial Contamination of Orthodontic Arch wire |
title_full | Assessment of Bacterial Contamination of Orthodontic Arch wire |
title_fullStr | Assessment of Bacterial Contamination of Orthodontic Arch wire |
title_full_unstemmed | Assessment of Bacterial Contamination of Orthodontic Arch wire |
title_short | Assessment of Bacterial Contamination of Orthodontic Arch wire |
title_sort | assessment of bacterial contamination of orthodontic arch wire |
url | https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/2578 |
work_keys_str_mv | AT suhashassan assessmentofbacterialcontaminationoforthodonticarchwire AT nidhalhghaib assessmentofbacterialcontaminationoforthodonticarchwire AT batoolhalghurabi assessmentofbacterialcontaminationoforthodonticarchwire |