Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf
Chlorophyll (Chl)-deficient plants can potentially increase global surface albedo of mono-cropping systems, and simultaneously maintain a similar photosynthetic efficiency by increasing light canopy penetration and thus lowering investment in pigments. However, some previous studies have shown that...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469022000458 |
| _version_ | 1811172236056330240 |
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| author | Kelvin Acebron Nicole Salvatori Giorgio Alberti Onno Muller Alessandro Peressotti Uwe Rascher Shizue Matsubara |
| author_facet | Kelvin Acebron Nicole Salvatori Giorgio Alberti Onno Muller Alessandro Peressotti Uwe Rascher Shizue Matsubara |
| author_sort | Kelvin Acebron |
| collection | DOAJ |
| description | Chlorophyll (Chl)-deficient plants can potentially increase global surface albedo of mono-cropping systems, and simultaneously maintain a similar photosynthetic efficiency by increasing light canopy penetration and thus lowering investment in pigments. However, some previous studies have shown that pale mutants might reduce productivity in field conditions. Such lower yields were suspected to be due to loss of photosynthetic efficiency at leaf level during light fluctuations as a consequence of reduced capacity and slower relaxation of non-photochemical quenching (NPQ) of Chl fluorescence. In this paper, we tested this hypothesis by comparing, CO2 assimilation (A), photosystem II (PSII) efficiency (ΦPSII), photochemical quenching and NPQ, electron transport rate (ETR) and fluorescence yield (Fyield) in a green soybean (Glycine max L.) cultivar (Eiko) and in a Chl-deficient (MinnGold) mutant under dynamically fluctuating light conditions. MinnGold had significantly slower induction of ETR and lower A and ETR than Eiko, but there was little difference in ΦPSII between the two genotypes, suggesting that the lower photosynthesis of MinnGold was mainly due to lower light energy absorption by a Chl-deficient leaf. The NPQ capacity was also smaller in MinnGold than in Eiko. As for the kinetics of the rapidly inducible component of NPQ, MinnGold showed slower induction, not relaxation, than Eiko. The combination of the effect of Chl-deficiency on lower photosynthesis, NPQ capacity and slower NPQ induction may explain the lower biomass accumulation of MinnGold in the field. Our physiological observations, combined with fluorescence kinetics, can serve as a basis to parameterize Chl content in modelling radiative transfer and photosynthesis for upscaling measures of plant and ecosystem productivity by a big leaf model. |
| first_indexed | 2024-04-10T17:26:30Z |
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| institution | Directory Open Access Journal |
| issn | 2666-4690 |
| language | English |
| last_indexed | 2024-04-10T17:26:30Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj.art-e5ae80c759ba480dbf7e2f00f5a48f7f2023-02-04T04:18:53ZengElsevierJournal of Photochemistry and Photobiology2666-46902023-02-0113100152Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leafKelvin Acebron0Nicole Salvatori1Giorgio Alberti2Onno Muller3Alessandro Peressotti4Uwe Rascher5Shizue Matsubara6Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, Leo-Brandt-Str, 52425, Jülich, Germany; Corresponding author.Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206, 33100, Udine, Italy; Department of Life Sciences, University of Trieste, 34127, Trieste, ItalyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206, 33100, Udine, Italy; Faculty of Science and Technology, Free University of Bolzano, Bolzano, ItalyInstitute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, Leo-Brandt-Str, 52425, Jülich, GermanyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206, 33100, Udine, ItalyInstitute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, Leo-Brandt-Str, 52425, Jülich, GermanyInstitute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, Leo-Brandt-Str, 52425, Jülich, GermanyChlorophyll (Chl)-deficient plants can potentially increase global surface albedo of mono-cropping systems, and simultaneously maintain a similar photosynthetic efficiency by increasing light canopy penetration and thus lowering investment in pigments. However, some previous studies have shown that pale mutants might reduce productivity in field conditions. Such lower yields were suspected to be due to loss of photosynthetic efficiency at leaf level during light fluctuations as a consequence of reduced capacity and slower relaxation of non-photochemical quenching (NPQ) of Chl fluorescence. In this paper, we tested this hypothesis by comparing, CO2 assimilation (A), photosystem II (PSII) efficiency (ΦPSII), photochemical quenching and NPQ, electron transport rate (ETR) and fluorescence yield (Fyield) in a green soybean (Glycine max L.) cultivar (Eiko) and in a Chl-deficient (MinnGold) mutant under dynamically fluctuating light conditions. MinnGold had significantly slower induction of ETR and lower A and ETR than Eiko, but there was little difference in ΦPSII between the two genotypes, suggesting that the lower photosynthesis of MinnGold was mainly due to lower light energy absorption by a Chl-deficient leaf. The NPQ capacity was also smaller in MinnGold than in Eiko. As for the kinetics of the rapidly inducible component of NPQ, MinnGold showed slower induction, not relaxation, than Eiko. The combination of the effect of Chl-deficiency on lower photosynthesis, NPQ capacity and slower NPQ induction may explain the lower biomass accumulation of MinnGold in the field. Our physiological observations, combined with fluorescence kinetics, can serve as a basis to parameterize Chl content in modelling radiative transfer and photosynthesis for upscaling measures of plant and ecosystem productivity by a big leaf model.http://www.sciencedirect.com/science/article/pii/S2666469022000458Non-photochemical quenching (NPQ)Photochemical quenchingEffective quantum yield of photosystem II (ΦPSII)Electron transport rate (ETR)Solar-induced fluorescence (SIF) |
| spellingShingle | Kelvin Acebron Nicole Salvatori Giorgio Alberti Onno Muller Alessandro Peressotti Uwe Rascher Shizue Matsubara Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf Journal of Photochemistry and Photobiology Non-photochemical quenching (NPQ) Photochemical quenching Effective quantum yield of photosystem II (ΦPSII) Electron transport rate (ETR) Solar-induced fluorescence (SIF) |
| title | Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf |
| title_full | Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf |
| title_fullStr | Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf |
| title_full_unstemmed | Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf |
| title_short | Elucidating the photosynthetic responses in chlorophyll-deficient soybean (Glycine max, L.) leaf |
| title_sort | elucidating the photosynthetic responses in chlorophyll deficient soybean glycine max l leaf |
| topic | Non-photochemical quenching (NPQ) Photochemical quenching Effective quantum yield of photosystem II (ΦPSII) Electron transport rate (ETR) Solar-induced fluorescence (SIF) |
| url | http://www.sciencedirect.com/science/article/pii/S2666469022000458 |
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