Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i>
Marine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/155 |
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| author | Kuan-Kuan Yuan Zi-Min Chen Ya-Xin Liu Hong-Ye Li Wei-Dong Yang |
| author_facet | Kuan-Kuan Yuan Zi-Min Chen Ya-Xin Liu Hong-Ye Li Wei-Dong Yang |
| author_sort | Kuan-Kuan Yuan |
| collection | DOAJ |
| description | Marine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effect of DHA on the response of the bivalve, <i>Perna viridis</i>, to DSTs by using LC-MS/MS, RT-qPCR, and histological examination. In this study, we observed that the DHA content decreased significantly with esterification of DSTs in the digestive gland of the mussel <i>P. viridis</i> after 96 h of exposure to <i>Prorocentrum lima</i>, a DST-producing dinoflagellate. The addition of DHA significantly increased the esterification level of DSTs and increased the expression of Nrf2 signaling pathway-related genes and enzyme activities, alleviating the damage of DSTs to digestive glands. These results suggested that DHA may mediate the esterification of DSTs and activation of the Nrf2 signaling pathway in <i>P. viridis</i> to protect mussels from the toxic effects of DSTs. This study may provide new insights regarding the response of bivalves to DSTs and lay the foundation for uncovering the role of DHA in environmental adaptation of bivalves. |
| first_indexed | 2024-03-11T06:16:44Z |
| format | Article |
| id | doaj.art-e5af4cf28cd747ed83182e7c19bdf273 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:44Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e5af4cf28cd747ed83182e7c19bdf2732023-11-17T12:17:18ZengMDPI AGMarine Drugs1660-33972023-02-0121315510.3390/md21030155Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i>Kuan-Kuan Yuan0Zi-Min Chen1Ya-Xin Liu2Hong-Ye Li3Wei-Dong Yang4Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaKey Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaKey Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaKey Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaKey Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaMarine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effect of DHA on the response of the bivalve, <i>Perna viridis</i>, to DSTs by using LC-MS/MS, RT-qPCR, and histological examination. In this study, we observed that the DHA content decreased significantly with esterification of DSTs in the digestive gland of the mussel <i>P. viridis</i> after 96 h of exposure to <i>Prorocentrum lima</i>, a DST-producing dinoflagellate. The addition of DHA significantly increased the esterification level of DSTs and increased the expression of Nrf2 signaling pathway-related genes and enzyme activities, alleviating the damage of DSTs to digestive glands. These results suggested that DHA may mediate the esterification of DSTs and activation of the Nrf2 signaling pathway in <i>P. viridis</i> to protect mussels from the toxic effects of DSTs. This study may provide new insights regarding the response of bivalves to DSTs and lay the foundation for uncovering the role of DHA in environmental adaptation of bivalves.https://www.mdpi.com/1660-3397/21/3/155diarrhetic shellfish toxinsDHAesterificationNrf2 signaling pathwayqPCRhistopathology |
| spellingShingle | Kuan-Kuan Yuan Zi-Min Chen Ya-Xin Liu Hong-Ye Li Wei-Dong Yang Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> Marine Drugs diarrhetic shellfish toxins DHA esterification Nrf2 signaling pathway qPCR histopathology |
| title | Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> |
| title_full | Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> |
| title_fullStr | Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> |
| title_full_unstemmed | Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> |
| title_short | Possible Role of Docosahexaenoic Acid in Response to Diarrhetic Shellfish Toxins in the Mussel <i>Perna viridis</i> |
| title_sort | possible role of docosahexaenoic acid in response to diarrhetic shellfish toxins in the mussel i perna viridis i |
| topic | diarrhetic shellfish toxins DHA esterification Nrf2 signaling pathway qPCR histopathology |
| url | https://www.mdpi.com/1660-3397/21/3/155 |
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