Contrast-enhanced fluorescence microscope by LED integrated excitation cubes
Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world, and the fluorescence excitation light source is one of the most critical components. To compensate for the short operation lifetime, integrated light sources, and low excitation efficiency of conventional lig...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Light Publishing Group
2023-02-01
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| Series: | Light: Advanced Manufacturing |
| Subjects: | |
| Online Access: | https://www.light-am.com/article/doi/10.37188/lam.2023.008 |
| _version_ | 1797847528405729280 |
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| author | Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo Dayong Jin |
| author_facet | Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo Dayong Jin |
| author_sort | Yuanhua Liu |
| collection | DOAJ |
| description | Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world, and the fluorescence excitation light source is one of the most critical components. To compensate for the short operation lifetime, integrated light sources, and low excitation efficiency of conventional light sources such as mercury, halogen, and xenon lamps, we designed an LED-integrated excitation cube (LEC) with a decentralized structure and high optical power density. Using a Fresnel lens, the light from the light-emitting diode (LED) was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination. LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes. LECs’ optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps; therefore, high-quality fluorescence imaging with spectral coverage from UV to red can be achieved. By replacing conventional fluorescence filter cubes, LEC can be easily installed on any commercial fluorescence microscope. A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses. Moreover, high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence. |
| first_indexed | 2024-04-09T18:12:55Z |
| format | Article |
| id | doaj.art-e5b03031430b488980227e1f71eb486d |
| institution | Directory Open Access Journal |
| issn | 2689-9620 |
| language | English |
| last_indexed | 2024-04-09T18:12:55Z |
| publishDate | 2023-02-01 |
| publisher | Light Publishing Group |
| record_format | Article |
| series | Light: Advanced Manufacturing |
| spelling | doaj.art-e5b03031430b488980227e1f71eb486d2023-04-13T13:21:57ZengLight Publishing GroupLight: Advanced Manufacturing2689-96202023-02-014111010.37188/lam.2023.008Contrast-enhanced fluorescence microscope by LED integrated excitation cubesYuanhua Liu0Xiang Zhang1Fei SuZhiyong Guo2Dayong JinUTS-SUSTech Joint Research Centre for Biomedical Materials and Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, ChinaUTS-SUSTech Joint Research Centre for Biomedical Materials and Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, ChinaUTS-SUSTech Joint Research Centre for Biomedical Materials and Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, ChinaFluorescence microscopy is a powerful tool for scientists to observe the microscopic world, and the fluorescence excitation light source is one of the most critical components. To compensate for the short operation lifetime, integrated light sources, and low excitation efficiency of conventional light sources such as mercury, halogen, and xenon lamps, we designed an LED-integrated excitation cube (LEC) with a decentralized structure and high optical power density. Using a Fresnel lens, the light from the light-emitting diode (LED) was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination. LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes. LECs’ optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps; therefore, high-quality fluorescence imaging with spectral coverage from UV to red can be achieved. By replacing conventional fluorescence filter cubes, LEC can be easily installed on any commercial fluorescence microscope. A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses. Moreover, high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.https://www.light-am.com/article/doi/10.37188/lam.2023.008led-integrated excitation cubefilter cubetime-gated imagingfluorescence microscopy |
| spellingShingle | Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo Dayong Jin Contrast-enhanced fluorescence microscope by LED integrated excitation cubes Light: Advanced Manufacturing led-integrated excitation cube filter cube time-gated imaging fluorescence microscopy |
| title | Contrast-enhanced fluorescence microscope by LED integrated excitation cubes |
| title_full | Contrast-enhanced fluorescence microscope by LED integrated excitation cubes |
| title_fullStr | Contrast-enhanced fluorescence microscope by LED integrated excitation cubes |
| title_full_unstemmed | Contrast-enhanced fluorescence microscope by LED integrated excitation cubes |
| title_short | Contrast-enhanced fluorescence microscope by LED integrated excitation cubes |
| title_sort | contrast enhanced fluorescence microscope by led integrated excitation cubes |
| topic | led-integrated excitation cube filter cube time-gated imaging fluorescence microscopy |
| url | https://www.light-am.com/article/doi/10.37188/lam.2023.008 |
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