<i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways
More and more research in dermatology and cosmetic science is devoted to the development and application of postbiotic raw materials. In order to explore the anti-photoaging efficacy and application prospect of <i>Bacillus amyloliquefaciens</i> lysate (BAL1) on the skin, we used 16 J/cm&...
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MDPI AG
2022-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/18/9151 |
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| author | Yongtao Zhang Jingsha Zhao Yanbing Jiang Dongdong Wang Dan Zhao Changtao Wang Meng Li |
| author_facet | Yongtao Zhang Jingsha Zhao Yanbing Jiang Dongdong Wang Dan Zhao Changtao Wang Meng Li |
| author_sort | Yongtao Zhang |
| collection | DOAJ |
| description | More and more research in dermatology and cosmetic science is devoted to the development and application of postbiotic raw materials. In order to explore the anti-photoaging efficacy and application prospect of <i>Bacillus amyloliquefaciens</i> lysate (BAL1) on the skin, we used 16 J/cm<sup>2</sup> UVA stimulation of human embryonic fibroblasts (CCC-ESF-1) to establish a UVA photodamage model to investigate the anti-photoaging efficacy of BAL1 and its mechanism of action. In this study, we found that BAL1 activated the transcription of downstream antioxidant enzyme genes mainly by promoting the nuclear displacement of NF-E2-related factor 2 (Nrf2) within CCC-ESF-1, thus increasing the antioxidant capacity of antioxidant enzymes to scavenge excessive reactive oxygen species in cells. Meanwhile, BAL1 promoted intracellular TGF-β/Smad signaling pathway and reduced matrix metalloproteinase expression to alleviate the degradation of extracellular matrix. In conclusion, the results of this study demonstrate the potential benefit of BAL1 in protecting the skin against UVA-induced photoaging and highlight the potential of BAL1 in skin photoprotection. |
| first_indexed | 2024-03-10T00:49:27Z |
| format | Article |
| id | doaj.art-e5b377d9704740a29e07f22d6e539142 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T00:49:27Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-e5b377d9704740a29e07f22d6e5391422023-11-23T14:53:50ZengMDPI AGApplied Sciences2076-34172022-09-011218915110.3390/app12189151<i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling PathwaysYongtao Zhang0Jingsha Zhao1Yanbing Jiang2Dongdong Wang3Dan Zhao4Changtao Wang5Meng Li6Beijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, ChinaMore and more research in dermatology and cosmetic science is devoted to the development and application of postbiotic raw materials. In order to explore the anti-photoaging efficacy and application prospect of <i>Bacillus amyloliquefaciens</i> lysate (BAL1) on the skin, we used 16 J/cm<sup>2</sup> UVA stimulation of human embryonic fibroblasts (CCC-ESF-1) to establish a UVA photodamage model to investigate the anti-photoaging efficacy of BAL1 and its mechanism of action. In this study, we found that BAL1 activated the transcription of downstream antioxidant enzyme genes mainly by promoting the nuclear displacement of NF-E2-related factor 2 (Nrf2) within CCC-ESF-1, thus increasing the antioxidant capacity of antioxidant enzymes to scavenge excessive reactive oxygen species in cells. Meanwhile, BAL1 promoted intracellular TGF-β/Smad signaling pathway and reduced matrix metalloproteinase expression to alleviate the degradation of extracellular matrix. In conclusion, the results of this study demonstrate the potential benefit of BAL1 in protecting the skin against UVA-induced photoaging and highlight the potential of BAL1 in skin photoprotection.https://www.mdpi.com/2076-3417/12/18/9151<i>Bacillus amyloliquefaciens</i>postbioticslysateNrf2antioxidantanti-photoaging |
| spellingShingle | Yongtao Zhang Jingsha Zhao Yanbing Jiang Dongdong Wang Dan Zhao Changtao Wang Meng Li <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways Applied Sciences <i>Bacillus amyloliquefaciens</i> postbiotics lysate Nrf2 antioxidant anti-photoaging |
| title | <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways |
| title_full | <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways |
| title_fullStr | <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways |
| title_full_unstemmed | <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways |
| title_short | <i>Bacillus amyloliquefaciens</i> Lysate Ameliorates Photoaging of Human Skin Fibroblasts through NRF2/KEAP1 and TGF-β/SMAD Signaling Pathways |
| title_sort | i bacillus amyloliquefaciens i lysate ameliorates photoaging of human skin fibroblasts through nrf2 keap1 and tgf β smad signaling pathways |
| topic | <i>Bacillus amyloliquefaciens</i> postbiotics lysate Nrf2 antioxidant anti-photoaging |
| url | https://www.mdpi.com/2076-3417/12/18/9151 |
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