Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome
The chloroplast, the energy organelle unique to plants and green algae, performs many functions, including photosynthesis and biosynthesis of metabolites. However, as the most critical tuber crop worldwide, the chloroplast proteome of potato (<i>Solanum tuberosum</i>) has not been explor...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/15/1915 |
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| author | Shengxuan Liu Tengfei Liu Enshuang Wang Yunxia Cheng Tiantian Liu Guogang Chen Minrui Guo Botao Song |
| author_facet | Shengxuan Liu Tengfei Liu Enshuang Wang Yunxia Cheng Tiantian Liu Guogang Chen Minrui Guo Botao Song |
| author_sort | Shengxuan Liu |
| collection | DOAJ |
| description | The chloroplast, the energy organelle unique to plants and green algae, performs many functions, including photosynthesis and biosynthesis of metabolites. However, as the most critical tuber crop worldwide, the chloroplast proteome of potato (<i>Solanum tuberosum</i>) has not been explored. Here, we use Percoll density gradient centrifugation to isolate intact chloroplasts from leaves of potato cultivar E3 and establish a reference proteome map of potato chloroplast by bottom-up proteomics. A total of 1834 non-redundant proteins were identified in the chloroplast proteome, including 51 proteins encoded by the chloroplast genome. Extensive sequence-based localization prediction revealed over 62% of proteins to be chloroplast resident by at least one algorithm. Sixteen proteins were selected to evaluate the prediction result by transient fluorescence assay, which confirmed that 14 were distributed in distinct internal compartments of the chloroplast. In addition, we identified 136 phosphorylation sites in 61 proteins encoded by chloroplast proteome. Furthermore, we reconstruct the snapshots along starch metabolic pathways in the two different types of plastids by a comparative analysis between chloroplast and previously reported amyloplast proteomes. Altogether, our results establish a comprehensive proteome map with post-translationally modified sites of potato chloroplast, which would provide the theoretical principle for the research of the photosynthesis pathway and starch metabolism. |
| first_indexed | 2024-03-09T12:17:17Z |
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| id | doaj.art-e5b3a074014547b9beb4badf237482a2 |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:17:17Z |
| publishDate | 2022-07-01 |
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| series | Plants |
| spelling | doaj.art-e5b3a074014547b9beb4badf237482a22023-11-30T22:45:02ZengMDPI AGPlants2223-77472022-07-011115191510.3390/plants11151915Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast ProteomeShengxuan Liu0Tengfei Liu1Enshuang Wang2Yunxia Cheng3Tiantian Liu4Guogang Chen5Minrui Guo6Botao Song7Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science, Tarim University, Alar 843300, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaSchool of Food Science and Technology, Shihezi University, Shihezi 832003, ChinaSchool of Food Science and Technology, Shihezi University, Shihezi 832003, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaThe chloroplast, the energy organelle unique to plants and green algae, performs many functions, including photosynthesis and biosynthesis of metabolites. However, as the most critical tuber crop worldwide, the chloroplast proteome of potato (<i>Solanum tuberosum</i>) has not been explored. Here, we use Percoll density gradient centrifugation to isolate intact chloroplasts from leaves of potato cultivar E3 and establish a reference proteome map of potato chloroplast by bottom-up proteomics. A total of 1834 non-redundant proteins were identified in the chloroplast proteome, including 51 proteins encoded by the chloroplast genome. Extensive sequence-based localization prediction revealed over 62% of proteins to be chloroplast resident by at least one algorithm. Sixteen proteins were selected to evaluate the prediction result by transient fluorescence assay, which confirmed that 14 were distributed in distinct internal compartments of the chloroplast. In addition, we identified 136 phosphorylation sites in 61 proteins encoded by chloroplast proteome. Furthermore, we reconstruct the snapshots along starch metabolic pathways in the two different types of plastids by a comparative analysis between chloroplast and previously reported amyloplast proteomes. Altogether, our results establish a comprehensive proteome map with post-translationally modified sites of potato chloroplast, which would provide the theoretical principle for the research of the photosynthesis pathway and starch metabolism.https://www.mdpi.com/2223-7747/11/15/1915proteomicschloroplastamyloplastphotosynthesisstarch metabolism<i>Solanum tuberosum</i> |
| spellingShingle | Shengxuan Liu Tengfei Liu Enshuang Wang Yunxia Cheng Tiantian Liu Guogang Chen Minrui Guo Botao Song Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome Plants proteomics chloroplast amyloplast photosynthesis starch metabolism <i>Solanum tuberosum</i> |
| title | Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome |
| title_full | Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome |
| title_fullStr | Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome |
| title_full_unstemmed | Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome |
| title_short | Dissecting the Chloroplast Proteome of the Potato (<i>Solanum Tuberosum</i> L.) and Its Comparison with the Tuber Amyloplast Proteome |
| title_sort | dissecting the chloroplast proteome of the potato i solanum tuberosum i l and its comparison with the tuber amyloplast proteome |
| topic | proteomics chloroplast amyloplast photosynthesis starch metabolism <i>Solanum tuberosum</i> |
| url | https://www.mdpi.com/2223-7747/11/15/1915 |
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