Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens
ABSTRACTSex is a major biological factor in the development and physiology of a sexual reproductive organism, and its role in the growing process is needed to be investigated in various species. We compare blood transcriptome between 5 males and 5 females in 4-week-old Rhode Island Red chickens and...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-11-01
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| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2022.2146187 |
| _version_ | 1797815470275952640 |
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| author | Hana Kim Hyojun Choo Jihye Cha Myoungjin Jang Juhwan Son Taejoon Jeong Bong-Hwan Choi Youngjo Lim Han-Ha Chai Jungjae Lee Dajeong Lim Donghyun Shin Woncheoul Park Jong-Eun Park |
| author_facet | Hana Kim Hyojun Choo Jihye Cha Myoungjin Jang Juhwan Son Taejoon Jeong Bong-Hwan Choi Youngjo Lim Han-Ha Chai Jungjae Lee Dajeong Lim Donghyun Shin Woncheoul Park Jong-Eun Park |
| author_sort | Hana Kim |
| collection | DOAJ |
| description | ABSTRACTSex is a major biological factor in the development and physiology of a sexual reproductive organism, and its role in the growing process is needed to be investigated in various species. We compare blood transcriptome between 5 males and 5 females in 4-week-old Rhode Island Red chickens and perform functional annotation of differentially expressed genes (DEGs). The results are as follows. 141 and 109 DEGs were located in autosomes and sex chromosomes, respectively. The gene ontology (GO) terms are significantly (p < 0.05) enriched, which were limb development, inner ear development, positive regulation of dendrite development, the KEGG pathway the TGF-beta signaling pathway, and melanogenesis (p < 0.05). These pathways are related to morphological maintenance and growth of the tissues. In addition, the SMAD2W and the BMP5 were involved in the TGF-beta signaling pathway, and both play an important role in maintaining tissue development. The major DEGs related to the development of neurons and synapses include the up-regulated NRN1, GDF10, SLC1A1, BMP5, NBEA, and NRXN1. Also, 7 DEGs were validated using RT-qPCR with high correlation (r2 = 0.74). In conclusion, the differential expression of blood tissue in the early growing chicken was enriched in TGF-beta signaling and related to the development of neurons and synapses including SMAD2W and BMP5. These results suggest that blood in the early growing stage is differentially affected in tissue development, nervous system, and pigmentation by sex. For future research, experimental characterization of DEGs and a holistic investigation of various tissues and growth stages will be required. |
| first_indexed | 2024-03-13T08:23:13Z |
| format | Article |
| id | doaj.art-e5b77914efe641f385f189501427ea36 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-03-13T08:23:13Z |
| publishDate | 2022-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-e5b77914efe641f385f189501427ea362023-05-31T05:33:13ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852022-11-0126635836810.1080/19768354.2022.2146187Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickensHana Kim0Hyojun Choo1Jihye Cha2Myoungjin Jang3Juhwan Son4Taejoon Jeong5Bong-Hwan Choi6Youngjo Lim7Han-Ha Chai8Jungjae Lee9Dajeong Lim10Donghyun Shin11Woncheoul Park12Jong-Eun Park13Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaPoultry Research Institute, National Institute of Animal Science, Pyeongchang, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDepartment of Animal Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDepartment of Agricultural Convergence Technology, Jeonbuk National University, Jeonju, KoreaDivision of Animal Genomics and Bioinformatics, National Institute of Animal Science, Wanju, KoreaDepartment of Animal Biotechnology, College of Applied Life Science, Jeju National University, Jeju-si, KoreaABSTRACTSex is a major biological factor in the development and physiology of a sexual reproductive organism, and its role in the growing process is needed to be investigated in various species. We compare blood transcriptome between 5 males and 5 females in 4-week-old Rhode Island Red chickens and perform functional annotation of differentially expressed genes (DEGs). The results are as follows. 141 and 109 DEGs were located in autosomes and sex chromosomes, respectively. The gene ontology (GO) terms are significantly (p < 0.05) enriched, which were limb development, inner ear development, positive regulation of dendrite development, the KEGG pathway the TGF-beta signaling pathway, and melanogenesis (p < 0.05). These pathways are related to morphological maintenance and growth of the tissues. In addition, the SMAD2W and the BMP5 were involved in the TGF-beta signaling pathway, and both play an important role in maintaining tissue development. The major DEGs related to the development of neurons and synapses include the up-regulated NRN1, GDF10, SLC1A1, BMP5, NBEA, and NRXN1. Also, 7 DEGs were validated using RT-qPCR with high correlation (r2 = 0.74). In conclusion, the differential expression of blood tissue in the early growing chicken was enriched in TGF-beta signaling and related to the development of neurons and synapses including SMAD2W and BMP5. These results suggest that blood in the early growing stage is differentially affected in tissue development, nervous system, and pigmentation by sex. For future research, experimental characterization of DEGs and a holistic investigation of various tissues and growth stages will be required.https://www.tandfonline.com/doi/10.1080/19768354.2022.2146187bloodtranscriptomesexchickenTGF-beta signaling pathway |
| spellingShingle | Hana Kim Hyojun Choo Jihye Cha Myoungjin Jang Juhwan Son Taejoon Jeong Bong-Hwan Choi Youngjo Lim Han-Ha Chai Jungjae Lee Dajeong Lim Donghyun Shin Woncheoul Park Jong-Eun Park Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens Animal Cells and Systems blood transcriptome sex chicken TGF-beta signaling pathway |
| title | Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens |
| title_full | Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens |
| title_fullStr | Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens |
| title_full_unstemmed | Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens |
| title_short | Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens |
| title_sort | blood transcriptome comparison between sexes and their function in 4 week rhode island red chickens |
| topic | blood transcriptome sex chicken TGF-beta signaling pathway |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2022.2146187 |
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