Involvement of nitric oxide in the effect of caffeine on the pentylenetetrazole-induced seizure threshold in mice
Background: Effect of caffeine on seizure is controversial in animal models of epilepsy. Caffeine is non-selective antagonist of A1 and A2A adenosine receptors. Meanwhile, nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate (NO-sGC-cGMP) pathway is partly involved in the central...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
| Published: |
Qazvin University of Medical Sciences & Health Services
2018-12-01
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| Series: | The Journal of Qazvin University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.qums.ac.ir/browse.php?a_id=2728&sid=1&slc_lang=en |
| Summary: | Background: Effect of caffeine on seizure is controversial in animal models of epilepsy. Caffeine
is non-selective antagonist of A1 and A2A adenosine receptors. Meanwhile, nitric oxide-soluble
guanylate cyclase-cyclic guanosine monophosphate (NO-sGC-cGMP) pathway is partly involved in
the central effects of caffeine.
Objective: The aim of this study was to investigate the effect of acute caffeine administration on
seizure threshold and levels of nitric oxide metabolites in pentylenetetrazole (PTZ) model in mice.
Methods: In this study, 35 NMRI mice (25-30 g) were divided into 5 groups (7 animals in each
group). Clonic seizure threshold induced by PTZ was assessed in intact control group or 30 min
after acute intraperitoneal administration of caffeine (5, 100, and 150 mg/kg) or saline. Nitric oxide
metabolites (NOx) were measured in the brain tissues of all groups at the end of experiments. Data
were analyzed by ANOVA and followed Tukey test for multiple comparisons. Findings: Acute administration of 5 mg/kg of caffeine significantly decreased seizure threshold
compared to saline (P<0.01). While 100 and 150 mg/kg of caffeine did not change seizure
threshold. NOx levels significantly decreased following acute administration of caffeine (100 and
150 mg/kg) compared to saline (P<0.05). Conclusion: The results of study showed that different doses of caffeine had different effects. It
seems that effect of higher doses of caffeine is partly mediated through modulation of nitric oxide
pathway. |
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| ISSN: | 1561-3666 2228-7213 |