High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria
Human enteroviruses (EVs) are highly prevalent in sewage and have been associated with human diseases with complications leading to severe neurological syndromes. We have used a recently developed molecular method to investigate the presence of EVs in eight samples collected in 2017–2018 from water...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/2/249 |
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| author | Manasi Majumdar Dimitra Klapsa Thomas Wilton Erika Bujaki Maria Dolores Fernandez-Garcia Temitope Oluwasegun Cephas Faleye Adefunke Olufunmilayo Oyero Moses Olubusuyi Adewumi Kader Ndiaye Johnson Adekunle Adeniji Javier Martin |
| author_facet | Manasi Majumdar Dimitra Klapsa Thomas Wilton Erika Bujaki Maria Dolores Fernandez-Garcia Temitope Oluwasegun Cephas Faleye Adefunke Olufunmilayo Oyero Moses Olubusuyi Adewumi Kader Ndiaye Johnson Adekunle Adeniji Javier Martin |
| author_sort | Manasi Majumdar |
| collection | DOAJ |
| description | Human enteroviruses (EVs) are highly prevalent in sewage and have been associated with human diseases with complications leading to severe neurological syndromes. We have used a recently developed molecular method to investigate the presence of EVs in eight samples collected in 2017–2018 from water streams contaminated by drainage channels in three different locations in Nigeria. A total of 93 human EV strains belonging to 45 different serotypes were identified, far exceeding the number of strains and serotypes found in similar samples in previous studies. Next generation sequencing analysis retrieved whole-capsid genomic nucleotide sequences of EV strains belonging to all four A, B, C, and D species. Our results further demonstrate the value of environmental surveillance for the detection of EV transmission of both serotypes commonly associated with clinical syndromes, such as EV-A71, and those that appear to circulate silently but could eventually cause outbreaks and disease. Several uncommon serotypes, rarely reported elsewhere, were detected such as EV-A119, EV-B87, EV-C116, and EV-D111. Ten EV serotypes were detected in Nigeria for the first time and two of them, CV-A12 and EV-B86, firstly described in Africa. This method can be expanded to generate whole-genome EV sequences as we show here for one EV-D111 strain. Our data revealed phylogenetic relationships of Nigerian sewage strains with EV strains reported elsewhere, mostly from African origin, and provided new insights into the whole-genome structure of emerging serotype EV-D111 and recombination events among EV-D serotypes. |
| first_indexed | 2024-03-09T05:26:47Z |
| format | Article |
| id | doaj.art-e5bf06f5456844c39d7eafad90ae53cd |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:26:47Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e5bf06f5456844c39d7eafad90ae53cd2023-12-03T12:36:35ZengMDPI AGViruses1999-49152021-02-0113224910.3390/v13020249High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in NigeriaManasi Majumdar0Dimitra Klapsa1Thomas Wilton2Erika Bujaki3Maria Dolores Fernandez-Garcia4Temitope Oluwasegun Cephas Faleye5Adefunke Olufunmilayo Oyero6Moses Olubusuyi Adewumi7Kader Ndiaye8Johnson Adekunle Adeniji9Javier Martin10Division of Virology, National Institute for Biological Standards and Control (NIBSC), Potters Bar EN6 3QG, Hertfordshire, UKDivision of Virology, National Institute for Biological Standards and Control (NIBSC), Potters Bar EN6 3QG, Hertfordshire, UKDivision of Virology, National Institute for Biological Standards and Control (NIBSC), Potters Bar EN6 3QG, Hertfordshire, UKDivision of Virology, National Institute for Biological Standards and Control (NIBSC), Potters Bar EN6 3QG, Hertfordshire, UKDepartment of Virology, Institute Pasteur, Dakar, SenegalDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, NigeriaWorld Health Organization National Polio Laboratory, Ibadan, Oyo State, NigeriaDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Virology, Institute Pasteur, Dakar, SenegalDepartment of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, NigeriaDivision of Virology, National Institute for Biological Standards and Control (NIBSC), Potters Bar EN6 3QG, Hertfordshire, UKHuman enteroviruses (EVs) are highly prevalent in sewage and have been associated with human diseases with complications leading to severe neurological syndromes. We have used a recently developed molecular method to investigate the presence of EVs in eight samples collected in 2017–2018 from water streams contaminated by drainage channels in three different locations in Nigeria. A total of 93 human EV strains belonging to 45 different serotypes were identified, far exceeding the number of strains and serotypes found in similar samples in previous studies. Next generation sequencing analysis retrieved whole-capsid genomic nucleotide sequences of EV strains belonging to all four A, B, C, and D species. Our results further demonstrate the value of environmental surveillance for the detection of EV transmission of both serotypes commonly associated with clinical syndromes, such as EV-A71, and those that appear to circulate silently but could eventually cause outbreaks and disease. Several uncommon serotypes, rarely reported elsewhere, were detected such as EV-A119, EV-B87, EV-C116, and EV-D111. Ten EV serotypes were detected in Nigeria for the first time and two of them, CV-A12 and EV-B86, firstly described in Africa. This method can be expanded to generate whole-genome EV sequences as we show here for one EV-D111 strain. Our data revealed phylogenetic relationships of Nigerian sewage strains with EV strains reported elsewhere, mostly from African origin, and provided new insights into the whole-genome structure of emerging serotype EV-D111 and recombination events among EV-D serotypes.https://www.mdpi.com/1999-4915/13/2/249environmental surveillancewastewaterhuman enterovirusnext generation sequencing (NGS)sewagerecombination |
| spellingShingle | Manasi Majumdar Dimitra Klapsa Thomas Wilton Erika Bujaki Maria Dolores Fernandez-Garcia Temitope Oluwasegun Cephas Faleye Adefunke Olufunmilayo Oyero Moses Olubusuyi Adewumi Kader Ndiaye Johnson Adekunle Adeniji Javier Martin High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria Viruses environmental surveillance wastewater human enterovirus next generation sequencing (NGS) sewage recombination |
| title | High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria |
| title_full | High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria |
| title_fullStr | High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria |
| title_full_unstemmed | High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria |
| title_short | High Diversity of Human Non-Polio Enterovirus Serotypes Identified in Contaminated Water in Nigeria |
| title_sort | high diversity of human non polio enterovirus serotypes identified in contaminated water in nigeria |
| topic | environmental surveillance wastewater human enterovirus next generation sequencing (NGS) sewage recombination |
| url | https://www.mdpi.com/1999-4915/13/2/249 |
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