Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress
Submergence stress is a limiting factor for direct-seeded rice systems in rainfed lowlands and flood-prone areas of South and Southeast Asia. The present study demonstrated that submergence stress severely hampered the germination and seedling growth of rice, however, seed priming alleviated the det...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01125/full |
| _version_ | 1818925484828786688 |
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| author | Saddam Hussain Hanqi Yin Shaobing Peng Faheem Ahmed Khan Fahad Khan Muhammad Sameeullah Hafiz Athar Hussain Jianliang Huang Kehui Cui Lixiao Nie |
| author_facet | Saddam Hussain Hanqi Yin Shaobing Peng Faheem Ahmed Khan Fahad Khan Muhammad Sameeullah Hafiz Athar Hussain Jianliang Huang Kehui Cui Lixiao Nie |
| author_sort | Saddam Hussain |
| collection | DOAJ |
| description | Submergence stress is a limiting factor for direct-seeded rice systems in rainfed lowlands and flood-prone areas of South and Southeast Asia. The present study demonstrated that submergence stress severely hampered the germination and seedling growth of rice, however, seed priming alleviated the detrimental effects of submergence stress. To elucidate the molecular basis of seed priming-induced submergence tolerance, transcriptome analyses were performed using 4-day-old primed (selenium-Se and salicylic acid-SA priming) and non-primed rice seedlings under submergence stress. Genomewide transcriptomic profiling identified 2371 and 2405 transcripts with Se- and SA-priming, respectively that were differentially expressed in rice compared with non-priming treatment under submergence. Pathway and gene ontology term enrichment analyses revealed that genes involved in regulation of secondary metabolism, development, cell, transport, protein and metal handling were over-represented after Se- or SA-priming. These coordinated factors might have enhanced the submergence tolerance and maintained the better germination and vigorous seedling growth of primed rice seedlings. It was also found that many genes involved in cellular and metabolic processes such as carbohydrate metabolism, cellular and metabolic biosynthesis, nitrogen compound metabolic process, transcription and response to oxidative stress were induced and overlapped in seed priming treatments, a finding which reveals the common mechanism of seed priming-induced submergence tolerance. Taken together, these results may provide new avenues for understanding and advancing priming-induced responses to submergence tolerance in crop plants. |
| first_indexed | 2024-12-20T02:41:58Z |
| format | Article |
| id | doaj.art-e5bf6c85ce2a4ccd91b32964dccc14bd |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T02:41:58Z |
| publishDate | 2016-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e5bf6c85ce2a4ccd91b32964dccc14bd2022-12-21T19:56:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-07-01710.3389/fpls.2016.01125197927Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence StressSaddam Hussain0Hanqi Yin1Shaobing Peng2Faheem Ahmed Khan3Fahad Khan4Muhammad Sameeullah5Hafiz Athar Hussain6Jianliang Huang7Kehui Cui8Lixiao Nie9Huazhong Agricultural UniversityShanghai Biotechnology CorporationHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityAbant Izzet Baysal UniversityUniversity of Agriculture, FaisalabadHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversitySubmergence stress is a limiting factor for direct-seeded rice systems in rainfed lowlands and flood-prone areas of South and Southeast Asia. The present study demonstrated that submergence stress severely hampered the germination and seedling growth of rice, however, seed priming alleviated the detrimental effects of submergence stress. To elucidate the molecular basis of seed priming-induced submergence tolerance, transcriptome analyses were performed using 4-day-old primed (selenium-Se and salicylic acid-SA priming) and non-primed rice seedlings under submergence stress. Genomewide transcriptomic profiling identified 2371 and 2405 transcripts with Se- and SA-priming, respectively that were differentially expressed in rice compared with non-priming treatment under submergence. Pathway and gene ontology term enrichment analyses revealed that genes involved in regulation of secondary metabolism, development, cell, transport, protein and metal handling were over-represented after Se- or SA-priming. These coordinated factors might have enhanced the submergence tolerance and maintained the better germination and vigorous seedling growth of primed rice seedlings. It was also found that many genes involved in cellular and metabolic processes such as carbohydrate metabolism, cellular and metabolic biosynthesis, nitrogen compound metabolic process, transcription and response to oxidative stress were induced and overlapped in seed priming treatments, a finding which reveals the common mechanism of seed priming-induced submergence tolerance. Taken together, these results may provide new avenues for understanding and advancing priming-induced responses to submergence tolerance in crop plants.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01125/fullGerminationtranscriptome analysisSubmergence stressSeed primingDirect-Seeded Rice |
| spellingShingle | Saddam Hussain Hanqi Yin Shaobing Peng Faheem Ahmed Khan Fahad Khan Muhammad Sameeullah Hafiz Athar Hussain Jianliang Huang Kehui Cui Lixiao Nie Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress Frontiers in Plant Science Germination transcriptome analysis Submergence stress Seed priming Direct-Seeded Rice |
| title | Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress |
| title_full | Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress |
| title_fullStr | Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress |
| title_full_unstemmed | Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress |
| title_short | Comparative Transcriptional Profiling of Primed and Non-Primed Rice Seedlings under Submergence Stress |
| title_sort | comparative transcriptional profiling of primed and non primed rice seedlings under submergence stress |
| topic | Germination transcriptome analysis Submergence stress Seed priming Direct-Seeded Rice |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01125/full |
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