Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells
Abstract In the present study, ZnO nanoparticles have been synthesized using an aqueous extract of shilajit. The nanoparticles were characterized using different techniques such as UV (ultraviolet–visible spectrophotometer), FTIR (Fourier transform infrared), XRD (X-ray diffraction), particle size a...
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| Format: | Article |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52217-x |
| _version_ | 1797276643995156480 |
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| author | Parthasarathi Perumal Nazeer Ahamed Sathakkathulla Kalaivani Kumaran Ramaladevi Ravikumar Justin Jayaraj Selvaraj Vijayakumar Nagendran Mariappan Gurusamy Naazneen Shaik Senthilkumar Gnanavadivel Prabhakaran Vinothkumar Suruli Palanichamy Vellaichamy Ganesan Purushoth Prabhu Thiraviam Seshan Gunalan Suresh Rathinasamy |
| author_facet | Parthasarathi Perumal Nazeer Ahamed Sathakkathulla Kalaivani Kumaran Ramaladevi Ravikumar Justin Jayaraj Selvaraj Vijayakumar Nagendran Mariappan Gurusamy Naazneen Shaik Senthilkumar Gnanavadivel Prabhakaran Vinothkumar Suruli Palanichamy Vellaichamy Ganesan Purushoth Prabhu Thiraviam Seshan Gunalan Suresh Rathinasamy |
| author_sort | Parthasarathi Perumal |
| collection | DOAJ |
| description | Abstract In the present study, ZnO nanoparticles have been synthesized using an aqueous extract of shilajit. The nanoparticles were characterized using different techniques such as UV (ultraviolet–visible spectrophotometer), FTIR (Fourier transform infrared), XRD (X-ray diffraction), particle size analysis, SEM (scanning electron microscope) and EDAX (Energy-dispersive X-ray) analysis. The UV absorption peak at 422.40 nm was observed for ZnO nanoparticles. SEM analysis showed the shape of nanoparticles to be spherical, FTIR spectrum confirmed the presence of zinc atoms, particle size analysis showed the nanoparticle size, EDAX confirmed the purity of ZnO nanoparticles whereas XRD pattern similar to that of JCPDS card for ZnO confirmed the presence of pure ZnO nanoparticles. The in vitro anticancer activity of ZnO nanoparticles against the HeLa cell line showed the IC50 value of 38.60 μg/mL compared to reference standard cisplatin. This finding confirms that ZnO nanoparticles from shilajit extract have potent cytotoxic effect on human cervical cancer cell lines. |
| first_indexed | 2024-03-07T15:31:11Z |
| format | Article |
| id | doaj.art-e5bf786889e84d1f910613b5dd418f50 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:31:11Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e5bf786889e84d1f910613b5dd418f502024-03-05T16:25:41ZengNature PortfolioScientific Reports2045-23222024-01-0114111110.1038/s41598-024-52217-xGreen synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cellsParthasarathi Perumal0Nazeer Ahamed Sathakkathulla1Kalaivani Kumaran2Ramaladevi Ravikumar3Justin Jayaraj Selvaraj4Vijayakumar Nagendran5Mariappan Gurusamy6Naazneen Shaik7Senthilkumar Gnanavadivel Prabhakaran8Vinothkumar Suruli Palanichamy9Vellaichamy Ganesan10Purushoth Prabhu Thiraviam11Seshan Gunalan12Suresh Rathinasamy13Department of Molecular and Cell Biology Lab, Greensmed LabsDepartment of Molecular and Cell Biology Lab, Greensmed LabsDepartment of Pharmaceutical Chemistry, EGS Pillay College of PharmacyDepartment of Pharmaceutical Chemistry, EGS Pillay College of PharmacyDepartment of Pharmaceutical Chemistry, EGS Pillay College of PharmacyDepartment of Pharmaceutical Chemistry, EGS Pillay College of PharmacyDepartment of Pharmaceutics, St. Mary’s College of PharmacyDepartment of Pharmaceutics, St. Mary’s College of PharmacyDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Bharath Institute of Higher Education and Research (BIHER)Department of Pharmaceutical Chemistry, Pannai College of Pharmacy (Affiliated to the Tamil Nadu Dr. M.G.R. Medical University, Chennai)Department of Pharmaceutical Chemistry, Pannai College of Pharmacy (Affiliated to the Tamil Nadu Dr. M.G.R. Medical University, Chennai)Department of Pharmacognosy, C. L. Baid Metha College of PharmacyCentre of Advanced Study in Crystallography and Biophysics, Guindy Campus, University of MadrasDepartment of Molecular and Cell Biology Lab, Greensmed LabsAbstract In the present study, ZnO nanoparticles have been synthesized using an aqueous extract of shilajit. The nanoparticles were characterized using different techniques such as UV (ultraviolet–visible spectrophotometer), FTIR (Fourier transform infrared), XRD (X-ray diffraction), particle size analysis, SEM (scanning electron microscope) and EDAX (Energy-dispersive X-ray) analysis. The UV absorption peak at 422.40 nm was observed for ZnO nanoparticles. SEM analysis showed the shape of nanoparticles to be spherical, FTIR spectrum confirmed the presence of zinc atoms, particle size analysis showed the nanoparticle size, EDAX confirmed the purity of ZnO nanoparticles whereas XRD pattern similar to that of JCPDS card for ZnO confirmed the presence of pure ZnO nanoparticles. The in vitro anticancer activity of ZnO nanoparticles against the HeLa cell line showed the IC50 value of 38.60 μg/mL compared to reference standard cisplatin. This finding confirms that ZnO nanoparticles from shilajit extract have potent cytotoxic effect on human cervical cancer cell lines.https://doi.org/10.1038/s41598-024-52217-x |
| spellingShingle | Parthasarathi Perumal Nazeer Ahamed Sathakkathulla Kalaivani Kumaran Ramaladevi Ravikumar Justin Jayaraj Selvaraj Vijayakumar Nagendran Mariappan Gurusamy Naazneen Shaik Senthilkumar Gnanavadivel Prabhakaran Vinothkumar Suruli Palanichamy Vellaichamy Ganesan Purushoth Prabhu Thiraviam Seshan Gunalan Suresh Rathinasamy Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells Scientific Reports |
| title | Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells |
| title_full | Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells |
| title_fullStr | Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells |
| title_full_unstemmed | Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells |
| title_short | Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against HeLa cells |
| title_sort | green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and their anticancer activity against hela cells |
| url | https://doi.org/10.1038/s41598-024-52217-x |
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