Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer
Abstract To test causal relationships between complex gut microbiota (GM) and host outcomes, researchers frequently transfer GM between donor and recipient mice via embryo transfer (ET) rederivation, cross-fostering (CF), and co-housing. In this study, we assess the influence of the transfer method...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24014-x |
| _version_ | 1811319619167715328 |
|---|---|
| author | Chunye Zhang Yushu Shi Matthew Burch Benjamin Olthoff Aaron C. Ericsson Craig L. Franklin |
| author_facet | Chunye Zhang Yushu Shi Matthew Burch Benjamin Olthoff Aaron C. Ericsson Craig L. Franklin |
| author_sort | Chunye Zhang |
| collection | DOAJ |
| description | Abstract To test causal relationships between complex gut microbiota (GM) and host outcomes, researchers frequently transfer GM between donor and recipient mice via embryo transfer (ET) rederivation, cross-fostering (CF), and co-housing. In this study, we assess the influence of the transfer method and the differences in baseline donor and recipient microbiota richness, on transfer efficiency. Additionally, recipient mice were subjected to DSS-induced chronic colitis to determine whether disease severity was affected by GM transfer efficiency or features within the GM. We found that the recipient’s genetic background, the baseline richness of donor and recipient GM, and the transfer method all influenced the GM transfer efficiency. Recipient genetic background and GM both had significant effects on DSS colitis severity and, unexpectedly, the transfer method was strongly associated with differential disease severity regardless of the other factors. |
| first_indexed | 2024-04-13T12:46:04Z |
| format | Article |
| id | doaj.art-e5c486b44ca74b9a85af76d39a7d9afe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T12:46:04Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e5c486b44ca74b9a85af76d39a7d9afe2022-12-22T02:46:22ZengNature PortfolioScientific Reports2045-23222022-11-0112111110.1038/s41598-022-24014-xTransfer efficiency and impact on disease phenotype of differing methods of gut microbiota transferChunye Zhang0Yushu Shi1Matthew Burch2Benjamin Olthoff3Aaron C. Ericsson4Craig L. Franklin5Department of Veterinary Pathobiology, University of MissouriDepartment of Statistics, University of MissouriDepartment of Veterinary Pathobiology, University of MissouriComparative Medicine Program, University of MissouriDepartment of Veterinary Pathobiology, University of MissouriDepartment of Veterinary Pathobiology, University of MissouriAbstract To test causal relationships between complex gut microbiota (GM) and host outcomes, researchers frequently transfer GM between donor and recipient mice via embryo transfer (ET) rederivation, cross-fostering (CF), and co-housing. In this study, we assess the influence of the transfer method and the differences in baseline donor and recipient microbiota richness, on transfer efficiency. Additionally, recipient mice were subjected to DSS-induced chronic colitis to determine whether disease severity was affected by GM transfer efficiency or features within the GM. We found that the recipient’s genetic background, the baseline richness of donor and recipient GM, and the transfer method all influenced the GM transfer efficiency. Recipient genetic background and GM both had significant effects on DSS colitis severity and, unexpectedly, the transfer method was strongly associated with differential disease severity regardless of the other factors.https://doi.org/10.1038/s41598-022-24014-x |
| spellingShingle | Chunye Zhang Yushu Shi Matthew Burch Benjamin Olthoff Aaron C. Ericsson Craig L. Franklin Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer Scientific Reports |
| title | Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| title_full | Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| title_fullStr | Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| title_full_unstemmed | Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| title_short | Transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| title_sort | transfer efficiency and impact on disease phenotype of differing methods of gut microbiota transfer |
| url | https://doi.org/10.1038/s41598-022-24014-x |
| work_keys_str_mv | AT chunyezhang transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer AT yushushi transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer AT matthewburch transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer AT benjaminolthoff transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer AT aaroncericsson transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer AT craiglfranklin transferefficiencyandimpactondiseasephenotypeofdifferingmethodsofgutmicrobiotatransfer |