Polyethylenimine-mediated Expression of Transgenes in the Acinar Cells of Rats Salivary Glands in vivo

Polyethylenimine-mediated Expression of Transgenes in the Acinar Cells of Rats Salivary Glands in vivo

Non viral-mediated transfection of plasmid DNA provides a fast and reliable way to express various transgenes in selected cell populations in live animals. Here, we show an improvement of a previously published method that is based on injecting plasmid DNA into the ductal system of the salivary glan...

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Bibliographic Details
Main Authors: Monika eSramkova, Laura eParente, Timothy eWigand, Myo-Pale eAye, Akiko eShitara, Roberto eWeigert
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
Acinar Cells
Aquaporin 5
Polyethyleneimine
Salivary Glands
intravital microscopy
In vivo Transfection
Online Access:http://journal.frontiersin.org/Journal/10.3389/fcell.2014.00074/full

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