Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification
Background: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expres...
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| Format: | Article |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.678770/full |
| _version_ | 1818752114022678528 |
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| author | Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song |
| author_facet | Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song |
| author_sort | Tao Jiang |
| collection | DOAJ |
| description | Background: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expression, and its relationship with clinicopathological features and prognosis in CRC, was analyzed by bioinformatic analysis and validated by clinical CRC specimen cohort. The functional characterization of QSOX2 was detected via in vitro and vivo experiments in CRC cell lines, while the potential signaling pathways were predicted by Gene Set Enrichment Analysis (GSEA).Results: Our data based on bioinformatical analysis and clinical validation demonstrated that the expression of QSOX2 in CRC tissues was significantly upregulated. Additionally, the chi-square test, logistic regression analysis, and Fisher’s exact test showed that QSOX2 overexpression was significantly correlated with advanced clinicopathological parameters, such as pathological stage and lymph node metastasis. The Kaplan–Meier curves and univariate Cox regression model showed that QSOX2 overexpression predicts poor overall survival (OS) and disease-free survival (DFS) in CRC patients. More importantly, multivariate Cox regression model showed that QSOX2 overexpression could serve as an independent factor for CRC patients. In vitro and vivo data showed that the proliferation and metastasis ability of CRC cells were suppressed on condition of QSOX2 inhibition. In addition, GSEA showed that the QSOX2 high expression phenotype has enriched multiple potential cancer-related signaling pathways.Conclusion: QSOX2 overexpression is strongly associated with malignant progression and poor oncological outcomes in CRC. QSOX2 might act as a novel biomarker for prognosis prediction and a new target for biotherapy in CRC. |
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| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-18T04:46:19Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-e5c6d7e2ef6f4bddb7151667b499880b2022-12-21T21:20:35ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-11-01910.3389/fcell.2021.678770678770Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical VerificationTao Jiang0Tao Jiang1Li Zheng2Li Zheng3Xia Li4Jia Liu5Hu Song6Hu Song7Yixin Xu8Yixin Xu9Chenhua Dong10Chenhua Dong11Lianyu Liu12Lianyu Liu13Hongyu Wang14Hongyu Wang15Shuai Wang16Renhao Wang17Renhao Wang18Jun Song19Jun Song20Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences, Beijing Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, ChinaThe Graduate School, Xuzhou Medical University, Xuzhou, ChinaDepartment of Pathology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaThe Graduate School, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaThe Graduate School, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaThe Graduate School, Xuzhou Medical University, Xuzhou, ChinaSchool of Life Sciences, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, ChinaDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, ChinaBackground: As a member of the atypical thiol oxidase family, quiescin sulfhydryl oxidase 2 (QSOX2) has been reported to play an important role in several biological processes, but the expression and function of QSOX2 in colorectal cancer (CRC) remains elusive.Methods: The difference of QSOX2 expression, and its relationship with clinicopathological features and prognosis in CRC, was analyzed by bioinformatic analysis and validated by clinical CRC specimen cohort. The functional characterization of QSOX2 was detected via in vitro and vivo experiments in CRC cell lines, while the potential signaling pathways were predicted by Gene Set Enrichment Analysis (GSEA).Results: Our data based on bioinformatical analysis and clinical validation demonstrated that the expression of QSOX2 in CRC tissues was significantly upregulated. Additionally, the chi-square test, logistic regression analysis, and Fisher’s exact test showed that QSOX2 overexpression was significantly correlated with advanced clinicopathological parameters, such as pathological stage and lymph node metastasis. The Kaplan–Meier curves and univariate Cox regression model showed that QSOX2 overexpression predicts poor overall survival (OS) and disease-free survival (DFS) in CRC patients. More importantly, multivariate Cox regression model showed that QSOX2 overexpression could serve as an independent factor for CRC patients. In vitro and vivo data showed that the proliferation and metastasis ability of CRC cells were suppressed on condition of QSOX2 inhibition. In addition, GSEA showed that the QSOX2 high expression phenotype has enriched multiple potential cancer-related signaling pathways.Conclusion: QSOX2 overexpression is strongly associated with malignant progression and poor oncological outcomes in CRC. QSOX2 might act as a novel biomarker for prognosis prediction and a new target for biotherapy in CRC.https://www.frontiersin.org/articles/10.3389/fcell.2021.678770/fullQSOX2colorectal cancerTCGATMAsprognosisGSEA |
| spellingShingle | Tao Jiang Tao Jiang Li Zheng Li Zheng Xia Li Jia Liu Hu Song Hu Song Yixin Xu Yixin Xu Chenhua Dong Chenhua Dong Lianyu Liu Lianyu Liu Hongyu Wang Hongyu Wang Shuai Wang Renhao Wang Renhao Wang Jun Song Jun Song Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification Frontiers in Cell and Developmental Biology QSOX2 colorectal cancer TCGA TMAs prognosis GSEA |
| title | Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification |
| title_full | Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification |
| title_fullStr | Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification |
| title_full_unstemmed | Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification |
| title_short | Quiescin Sulfhydryl Oxidase 2 Overexpression Predicts Poor Prognosis and Tumor Progression in Patients With Colorectal Cancer: A Study Based on Data Mining and Clinical Verification |
| title_sort | quiescin sulfhydryl oxidase 2 overexpression predicts poor prognosis and tumor progression in patients with colorectal cancer a study based on data mining and clinical verification |
| topic | QSOX2 colorectal cancer TCGA TMAs prognosis GSEA |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.678770/full |
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