Comparison of DNA Instability in Adenoma and Adenocarcinoma in Patients with Colorectal Cancer, Using Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)
Background: Colorectal cancer is the consequence of gathering numerous genetic alterations and it has been suggested that genomic instability is indispensable for the generation of multiple mutations underlying the development of cancer. Methods: Random amplified polymorphic DNA polymerase chain re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2017-07-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/7095 |
| Summary: | Background: Colorectal cancer is the consequence of gathering numerous genetic alterations and it has been suggested that genomic instability is indispensable for the generation of multiple mutations underlying the development of cancer.
Methods: Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method was utilized to find genomic alterations in adenomas and adenocarcinomas compared with normal epithelial tissue obtained from 17 patients with colorectal cancer.
Findings: Separated PCR products by 2% agarose determined approximate 370 base pairs band as a polymorphic fingerprint for normal (11.8%), adenoma (76.5%), and adenocarcinoma (88.2%) tissues. Polymorphic band could significantly discriminate adenomas [Odds ratio (OR) = 24.38, P = 0.0004] and adenocarcinomas (OR = 56.25, P < 0.0001) from normal tissues. Furthermore, the 370 base pairs band could not distinguish adenomas from adenocarcinomas (P = 0.6600). |
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| ISSN: | 1027-7595 1735-854X |