Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China
H7N7 avian influenza virus (AIV) can divided into low-pathogenic AIV and high-pathogenic AIV groups. It has been shown to infect humans and animals. Its prevalence state in wild birds in China remains largely unclear. In this study, a new strain of H7N7 AIV, designated CM1216, isolated from wild bir...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120308658 |
| _version_ | 1818736916926824448 |
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| author | Wangjun Tang Xuyong Li Ling Tang Tianhou Wang Guimei He |
| author_facet | Wangjun Tang Xuyong Li Ling Tang Tianhou Wang Guimei He |
| author_sort | Wangjun Tang |
| collection | DOAJ |
| description | H7N7 avian influenza virus (AIV) can divided into low-pathogenic AIV and high-pathogenic AIV groups. It has been shown to infect humans and animals. Its prevalence state in wild birds in China remains largely unclear. In this study, a new strain of H7N7 AIV, designated CM1216, isolated from wild birds in Shanghai, China, was characterized. Phylogenetic and nucleotide sequence analyses of CM1216 revealed that HA, NA, PB1, NP, and M genes shared the highest nucleotide identity with the Japan H7 subtype AIV circulated in 2019; the PB2 and PA genes shared the highest nucleotide identity with the Korea H7 subtype AIV circulated in wild birds in 2018, while NS gene of CM1216 was 98.93% identical to that of the duck AIV circulating in Bangladesh, and they all belong to the Eurasian lineage. A Bayesian phylogenetic reconstruction of the 2 surface genes of CM1216 showed that multiple reassortments might have occurred in 2015. Mutations were found in HA (A135 T, T136S, and T160 A [H3 numbering]), M1 (N30D and T215 A), NS1 (P42S and D97 E), PB2 (R389 K), and PA (N383D) proteins; these mutations have been shown to be related to mammalian adaptation and changes in virulence of AIVs. Infection studies demonstrated that CM1216 could infect mice and cause symptoms characteristic of influenza virus infection and proliferate in the lungs without prior adaption. This study demonstrates the need for routine surveillance of AIVs in wild birds and detection of their evolution to become a virus with high pathogenicity and ability to infect humans. |
| first_indexed | 2024-12-18T00:44:45Z |
| format | Article |
| id | doaj.art-e5c9c14e2fa34809a0cdbd69902c888f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-18T00:44:45Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-e5c9c14e2fa34809a0cdbd69902c888f2022-12-21T21:26:47ZengElsevierPoultry Science0032-57912021-02-011002565574Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, ChinaWangjun Tang0Xuyong Li1Ling Tang2Tianhou Wang3Guimei He4School of Life Sciences, East China Normal University, Shanghai, ChinaCollege of Agricultural, Liaocheng University, Liaocheng, ChinaSchool of Life Sciences, East China Normal University, Shanghai, ChinaSchool of Life Sciences, East China Normal University, Shanghai, China; Institute of Eco-Chongming (IEC), East China Normal University, Shanghai, ChinaSchool of Life Sciences, East China Normal University, Shanghai, China; Institute of Eco-Chongming (IEC), East China Normal University, Shanghai, China; Corresponding author:H7N7 avian influenza virus (AIV) can divided into low-pathogenic AIV and high-pathogenic AIV groups. It has been shown to infect humans and animals. Its prevalence state in wild birds in China remains largely unclear. In this study, a new strain of H7N7 AIV, designated CM1216, isolated from wild birds in Shanghai, China, was characterized. Phylogenetic and nucleotide sequence analyses of CM1216 revealed that HA, NA, PB1, NP, and M genes shared the highest nucleotide identity with the Japan H7 subtype AIV circulated in 2019; the PB2 and PA genes shared the highest nucleotide identity with the Korea H7 subtype AIV circulated in wild birds in 2018, while NS gene of CM1216 was 98.93% identical to that of the duck AIV circulating in Bangladesh, and they all belong to the Eurasian lineage. A Bayesian phylogenetic reconstruction of the 2 surface genes of CM1216 showed that multiple reassortments might have occurred in 2015. Mutations were found in HA (A135 T, T136S, and T160 A [H3 numbering]), M1 (N30D and T215 A), NS1 (P42S and D97 E), PB2 (R389 K), and PA (N383D) proteins; these mutations have been shown to be related to mammalian adaptation and changes in virulence of AIVs. Infection studies demonstrated that CM1216 could infect mice and cause symptoms characteristic of influenza virus infection and proliferate in the lungs without prior adaption. This study demonstrates the need for routine surveillance of AIVs in wild birds and detection of their evolution to become a virus with high pathogenicity and ability to infect humans.http://www.sciencedirect.com/science/article/pii/S0032579120308658H7N7avian influenza virusphylogeneticspathogenicitymouse |
| spellingShingle | Wangjun Tang Xuyong Li Ling Tang Tianhou Wang Guimei He Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China Poultry Science H7N7 avian influenza virus phylogenetics pathogenicity mouse |
| title | Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China |
| title_full | Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China |
| title_fullStr | Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China |
| title_full_unstemmed | Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China |
| title_short | Characterization of the low-pathogenic H7N7 avian influenza virus in Shanghai, China |
| title_sort | characterization of the low pathogenic h7n7 avian influenza virus in shanghai china |
| topic | H7N7 avian influenza virus phylogenetics pathogenicity mouse |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120308658 |
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