Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai
ObjectiveTo isolate and study the biological characteristics of severe acute respiratory syndrome coronavirus 2 （SARS-CoV-2） from feces of coronavirus disease 2019 （COVID-19） patients.MethodsVero E6 cells were used for virus isolation and the isolated strains were tested by nucleic acid test， immuno...
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| Format: | Article |
| Language: | zho |
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Shanghai Preventive Medicine Association
2022-12-01
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| Series: | Shanghai yufang yixue |
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| Online Access: | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.22266 |
| _version_ | 1811160901847351296 |
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| author | ZHOU Yanqiu CUI Xiaoxian MOU Jiabin FANG Fanghao TENG Zheng WU Huanyu CHEN Min ZHANG Xi |
| author_facet | ZHOU Yanqiu CUI Xiaoxian MOU Jiabin FANG Fanghao TENG Zheng WU Huanyu CHEN Min ZHANG Xi |
| author_sort | ZHOU Yanqiu |
| collection | DOAJ |
| description | ObjectiveTo isolate and study the biological characteristics of severe acute respiratory syndrome coronavirus 2 （SARS-CoV-2） from feces of coronavirus disease 2019 （COVID-19） patients.MethodsVero E6 cells were used for virus isolation and the isolated strains were tested by nucleic acid test， immunofluorescence test， virulence test and whole genome sequencing. 50% tissue culture infective dose （TCID50） was calculated after the cell cultures of each generation were collectedResultsEight fecal specimens were inoculated with Vero E6 cells after treatment and cultured for 48 h. One specimen showed obvious cytopathic effect on Vero E6 cells. One SARS-CoV-2 out of 8 fecal samples from COVID-19 patients were isolated， and separation rate was 12.5%. The TCID50 of P1， P2 and P3 were 104.0/0.2 mL， 104.5/0.2 mL and 104.75/0.2 mL， respectively. Only one of the 8 stool samples had SARS-CoV-2 virus replication and amplification， and the Ct value of the nucleic acid detection was about 10. The sequence of the isolation was more than 99.99% homologous with that of Wuhan-Hu-1（GenBank MN908947）.ConclusionThe SARS-CoV-2 strain is isolated from the fecal samples of COVID-19 cases and is confirmed by genomic sequencing and immunofluorescence test， which indicates the presence of live virus in feces of COVID-19 cases. |
| first_indexed | 2024-04-10T06:06:44Z |
| format | Article |
| id | doaj.art-e5c9e732b3f3498989bf482c817e4530 |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-04-10T06:06:44Z |
| publishDate | 2022-12-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-e5c9e732b3f3498989bf482c817e45302023-03-03T03:11:28ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312022-12-0134121173117910.19428/j.cnki.sjpm.2022.222661004-9231（2022）12-1173-07Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in ShanghaiZHOU Yanqiu0CUI Xiaoxian1MOU Jiabin2FANG Fanghao3TENG Zheng4WU Huanyu5CHEN Min6ZHANG Xi7Division of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaDivision of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaObjectiveTo isolate and study the biological characteristics of severe acute respiratory syndrome coronavirus 2 （SARS-CoV-2） from feces of coronavirus disease 2019 （COVID-19） patients.MethodsVero E6 cells were used for virus isolation and the isolated strains were tested by nucleic acid test， immunofluorescence test， virulence test and whole genome sequencing. 50% tissue culture infective dose （TCID50） was calculated after the cell cultures of each generation were collectedResultsEight fecal specimens were inoculated with Vero E6 cells after treatment and cultured for 48 h. One specimen showed obvious cytopathic effect on Vero E6 cells. One SARS-CoV-2 out of 8 fecal samples from COVID-19 patients were isolated， and separation rate was 12.5%. The TCID50 of P1， P2 and P3 were 104.0/0.2 mL， 104.5/0.2 mL and 104.75/0.2 mL， respectively. Only one of the 8 stool samples had SARS-CoV-2 virus replication and amplification， and the Ct value of the nucleic acid detection was about 10. The sequence of the isolation was more than 99.99% homologous with that of Wuhan-Hu-1（GenBank MN908947）.ConclusionThe SARS-CoV-2 strain is isolated from the fecal samples of COVID-19 cases and is confirmed by genomic sequencing and immunofluorescence test， which indicates the presence of live virus in feces of COVID-19 cases.http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.22266sars-cov-2fecesvero e6polymerase chain reactionwhole genome sequencing |
| spellingShingle | ZHOU Yanqiu CUI Xiaoxian MOU Jiabin FANG Fanghao TENG Zheng WU Huanyu CHEN Min ZHANG Xi Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai Shanghai yufang yixue sars-cov-2 feces vero e6 polymerase chain reaction whole genome sequencing |
| title | Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai |
| title_full | Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai |
| title_fullStr | Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai |
| title_full_unstemmed | Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai |
| title_short | Isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from COVID-19 patients in Shanghai |
| title_sort | isolation and identification of a severe acute respiratory syndrome coronavirus 2 strain in feces from covid 19 patients in shanghai |
| topic | sars-cov-2 feces vero e6 polymerase chain reaction whole genome sequencing |
| url | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.22266 |
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