Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants
The goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3145 |
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| author | Katarzyna Kasperkiewicz Roman Major Anna Sypien Marcin Kot Marcin Dyner Łukasz Major Adam Byrski Magdalena Kopernik Juergen M. Lackner |
| author_facet | Katarzyna Kasperkiewicz Roman Major Anna Sypien Marcin Kot Marcin Dyner Łukasz Major Adam Byrski Magdalena Kopernik Juergen M. Lackner |
| author_sort | Katarzyna Kasperkiewicz |
| collection | DOAJ |
| description | The goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy focused both on improvement of antibacterial properties against bacteria adhesion and on wear and corrosion resistance of tools for spine surgery. Further, a ~35% decrease in implant and tool dimensions was expected by introducing ultrahigh-strength titanium alloys for less-invasive surgeries. The tested materials, in the form of thin, multi-layered coatings, showed nanocrystalline microstructures. Performed direct-cytotoxicity studies (including lactate dehydrogenase activity measurement) showed that there was a low probability of adverse effects on surrounding SAOS-2 (<i>Homo sapiens</i> bone osteosarcoma) cells. The microbiological studies (e.g., ISO 22196 contact tests) showed that implanting Ag nanoparticles into Ti/Ti<sub>x</sub>N coatings inhibited the growth of <i>E. coli</i> and <i>S. aureus</i> cells and reduced their adhesion to the material surface. These findings suggest that Ag-nanoparticles present in implant coatings may potentially minimize infection risk and lower inherent stress. |
| first_indexed | 2024-03-10T11:05:51Z |
| format | Article |
| id | doaj.art-e5cab9d08bdf401ba0de4d91789a4a99 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:05:51Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e5cab9d08bdf401ba0de4d91789a4a992023-11-21T21:12:27ZengMDPI AGMolecules1420-30492021-05-012611314510.3390/molecules26113145Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal ImplantsKatarzyna Kasperkiewicz0Roman Major1Anna Sypien2Marcin Kot3Marcin Dyner4Łukasz Major5Adam Byrski6Magdalena Kopernik7Juergen M. Lackner8Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, PolandInstitute of Metallurgy and Materials Science, Polish Academy of Sciences, 30-059 Cracow, PolandInstitute of Metallurgy and Materials Science, Polish Academy of Sciences, 30-059 Cracow, PolandFaculty of Mechanical Engineering and Robotics, AGH University of Science and Technology, 30-059 Cracow, PolandFaculty of Science and Technology, Jan Dlugosz University in Czestochowa, 42-200 Czestochowa, PolandInstitute of Metallurgy and Materials Science, Polish Academy of Sciences, 30-059 Cracow, PolandInstitute of Metallurgy and Materials Science, Polish Academy of Sciences, 30-059 Cracow, PolandFaculty of Metals Engineering and Industrial Computer Science, AGH University of Science and Technology, 30-059 Cracow, PolandInstitute of Surface Technologies and Photonics, Functional Surfaces, Joanneum Research Forschungsges.m.b.H., 8712 Niklasdorf, AustriaThe goal of the work was to develop materials dedicated to spine surgery that minimized the potential for infection originating from the transfer of bacteria during long surgeries. The bacteria form biofilms, causing implant loosening, pain and finally, a risk of paralysis for patients. Our strategy focused both on improvement of antibacterial properties against bacteria adhesion and on wear and corrosion resistance of tools for spine surgery. Further, a ~35% decrease in implant and tool dimensions was expected by introducing ultrahigh-strength titanium alloys for less-invasive surgeries. The tested materials, in the form of thin, multi-layered coatings, showed nanocrystalline microstructures. Performed direct-cytotoxicity studies (including lactate dehydrogenase activity measurement) showed that there was a low probability of adverse effects on surrounding SAOS-2 (<i>Homo sapiens</i> bone osteosarcoma) cells. The microbiological studies (e.g., ISO 22196 contact tests) showed that implanting Ag nanoparticles into Ti/Ti<sub>x</sub>N coatings inhibited the growth of <i>E. coli</i> and <i>S. aureus</i> cells and reduced their adhesion to the material surface. These findings suggest that Ag-nanoparticles present in implant coatings may potentially minimize infection risk and lower inherent stress.https://www.mdpi.com/1420-3049/26/11/3145multilayer coatingsantimicrobial materialsbiomaterialsAg nanoparticlestitaniumspinal implants |
| spellingShingle | Katarzyna Kasperkiewicz Roman Major Anna Sypien Marcin Kot Marcin Dyner Łukasz Major Adam Byrski Magdalena Kopernik Juergen M. Lackner Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants Molecules multilayer coatings antimicrobial materials biomaterials Ag nanoparticles titanium spinal implants |
| title | Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants |
| title_full | Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants |
| title_fullStr | Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants |
| title_full_unstemmed | Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants |
| title_short | Antibacterial Optimization of Highly Deformed Titanium Alloys for Spinal Implants |
| title_sort | antibacterial optimization of highly deformed titanium alloys for spinal implants |
| topic | multilayer coatings antimicrobial materials biomaterials Ag nanoparticles titanium spinal implants |
| url | https://www.mdpi.com/1420-3049/26/11/3145 |
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