Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel
This paper challenges the production of the protein nanoparticles using the conjugation of Ni2+ complexed nitrilotriacetic acid end-functionalized polystyrene (Ni-NTA-PS) and histidine tagged GFP (His-GFP) hybrid. The microfluidic synthesis of the protein nanoparticle with the advantages of a unifor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-01-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/8/1/10 |
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| author | Hyeong Jin Jeon Chae Yeon Lee Moon Jeong Kim Xuan Don Nguyen Dong Hyeok Park Hyung Hoon Kim Jeung Sang Go Hyun-jong Paik |
| author_facet | Hyeong Jin Jeon Chae Yeon Lee Moon Jeong Kim Xuan Don Nguyen Dong Hyeok Park Hyung Hoon Kim Jeung Sang Go Hyun-jong Paik |
| author_sort | Hyeong Jin Jeon |
| collection | DOAJ |
| description | This paper challenges the production of the protein nanoparticles using the conjugation of Ni2+ complexed nitrilotriacetic acid end-functionalized polystyrene (Ni-NTA-PS) and histidine tagged GFP (His-GFP) hybrid. The microfluidic synthesis of the protein nanoparticle with the advantages of a uniform size, a fast reaction, and a precise control of preparation conditions is examined. The self-assembly occurs on the interfacial surface of the multi-laminated laminar flow stably formed in the microchannel. The clogging of the produced protein nanoparticles on the channel surface is solved by adding a retarding inlet channel. The size and shape of the produced protein nanoparticles are measured by the analysis of transmission electron microscopy (TEM) and scanning electron microscope (SEM) images, and the attachment of the protein is visualized with a green fluorescent image. Future research includes the encapsulation of vaccines and the coating of antigens on the protein surface. |
| first_indexed | 2024-12-10T21:37:23Z |
| format | Article |
| id | doaj.art-e5cae161da714cbfa4d744781faa4c9b |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-10T21:37:23Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-e5cae161da714cbfa4d744781faa4c9b2022-12-22T01:32:37ZengMDPI AGMicromachines2072-666X2017-01-01811010.3390/mi8010010mi8010010Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a MicrochannelHyeong Jin Jeon0Chae Yeon Lee1Moon Jeong Kim2Xuan Don Nguyen3Dong Hyeok Park4Hyung Hoon Kim5Jeung Sang Go6Hyun-jong Paik7School of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaDepartment of Polymer Science, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaSchool of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaSchool of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaSchool of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaBoditech Med Inc., 43, Geodudanji 1-gil, Dongnae-myeon, Chuncheon-si, Gang-won-do 24398, KoreaSchool of Mechanical Engineering, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaDepartment of Polymer Science, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, KoreaThis paper challenges the production of the protein nanoparticles using the conjugation of Ni2+ complexed nitrilotriacetic acid end-functionalized polystyrene (Ni-NTA-PS) and histidine tagged GFP (His-GFP) hybrid. The microfluidic synthesis of the protein nanoparticle with the advantages of a uniform size, a fast reaction, and a precise control of preparation conditions is examined. The self-assembly occurs on the interfacial surface of the multi-laminated laminar flow stably formed in the microchannel. The clogging of the produced protein nanoparticles on the channel surface is solved by adding a retarding inlet channel. The size and shape of the produced protein nanoparticles are measured by the analysis of transmission electron microscopy (TEM) and scanning electron microscope (SEM) images, and the attachment of the protein is visualized with a green fluorescent image. Future research includes the encapsulation of vaccines and the coating of antigens on the protein surface.http://www.mdpi.com/2072-666X/8/1/10protein nanoparticlesmicrofluidic synthesisself-assemblylamination flowprotein-polymer hybrid |
| spellingShingle | Hyeong Jin Jeon Chae Yeon Lee Moon Jeong Kim Xuan Don Nguyen Dong Hyeok Park Hyung Hoon Kim Jeung Sang Go Hyun-jong Paik Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel Micromachines protein nanoparticles microfluidic synthesis self-assembly lamination flow protein-polymer hybrid |
| title | Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel |
| title_full | Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel |
| title_fullStr | Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel |
| title_full_unstemmed | Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel |
| title_short | Preparation of Protein Nanoparticles Using NTA End Functionalized Polystyrenes on the Interface of a Multi-Laminated Flow Formed in a Microchannel |
| title_sort | preparation of protein nanoparticles using nta end functionalized polystyrenes on the interface of a multi laminated flow formed in a microchannel |
| topic | protein nanoparticles microfluidic synthesis self-assembly lamination flow protein-polymer hybrid |
| url | http://www.mdpi.com/2072-666X/8/1/10 |
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