SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases
Summary: The cellular targets of SARS-CoV-2, the novel coronavirus causing the COVID-19 pandemic, is still rudimentary. Here, we incorporated the protein information to analyze the expression of ACE2, the SARS-CoV-2 receptor, together with co-factors, TMPRSS2 and Furin, at single-cell level in situ,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422030941X |
| _version_ | 1828780942169210880 |
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| author | Lulin Zhou Zubiao Niu Xiaoyi Jiang Zhengrong Zhang You Zheng Zhongyi Wang Yichao Zhu Lihua Gao Hongyan Huang Xiaoning Wang Qiang Sun |
| author_facet | Lulin Zhou Zubiao Niu Xiaoyi Jiang Zhengrong Zhang You Zheng Zhongyi Wang Yichao Zhu Lihua Gao Hongyan Huang Xiaoning Wang Qiang Sun |
| author_sort | Lulin Zhou |
| collection | DOAJ |
| description | Summary: The cellular targets of SARS-CoV-2, the novel coronavirus causing the COVID-19 pandemic, is still rudimentary. Here, we incorporated the protein information to analyze the expression of ACE2, the SARS-CoV-2 receptor, together with co-factors, TMPRSS2 and Furin, at single-cell level in situ, which we called protein-proofed single-cell RNA (pscRNA) profiling. Systemic analysis across 36 tissues revealed a rank list of candidate cells potentially vulnerable to SARS-CoV-2. The top targets are lung AT2 cells and macrophages, then cardiomyocytes and adrenal gland stromal cells, followed by stromal cells in testis, ovary, and thyroid, whereas the kidney proximal tubule cells, cholangiocytes, and enterocytes are less likely to be the primary SARS-CoV-2 targets. Actually, the stomach may constitute a physical barrier against SARS-CoV-2 as the acidic environment (pH < 2.0) could completely inactivate SARS-CoV-2 pseudo-viruses. Together, we provide a comprehensive view on the potential SARS-CoV-2 targets by pscRNA profiling. |
| first_indexed | 2024-12-11T17:25:46Z |
| format | Article |
| id | doaj.art-e5cb0fe412754ace91b7ad44a80cca51 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-11T17:25:46Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e5cb0fe412754ace91b7ad44a80cca512022-12-22T00:56:59ZengElsevieriScience2589-00422020-11-012311101744SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin ProteasesLulin Zhou0Zubiao Niu1Xiaoyi Jiang2Zhengrong Zhang3You Zheng4Zhongyi Wang5Yichao Zhu6Lihua Gao7Hongyan Huang8Xiaoning Wang9Qiang Sun10Institute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China; School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, P. R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, 10 TIEYI Road, Beijing 100038, P. R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. ChinaDepartment of Oncology, Beijing Shijitan Hospital of Capital Medical University, 10 TIEYI Road, Beijing 100038, P. R. China; Corresponding authorSchool of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, P. R. China; School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, P. R. China; National Clinic Center of Geriatric, the Chinese PLA General Hospital, Beijing 100853, P. R. China; Corresponding authorInstitute of Biotechnology, 20 Dongda Street, Beijing 100071, P.R. China; Corresponding authorSummary: The cellular targets of SARS-CoV-2, the novel coronavirus causing the COVID-19 pandemic, is still rudimentary. Here, we incorporated the protein information to analyze the expression of ACE2, the SARS-CoV-2 receptor, together with co-factors, TMPRSS2 and Furin, at single-cell level in situ, which we called protein-proofed single-cell RNA (pscRNA) profiling. Systemic analysis across 36 tissues revealed a rank list of candidate cells potentially vulnerable to SARS-CoV-2. The top targets are lung AT2 cells and macrophages, then cardiomyocytes and adrenal gland stromal cells, followed by stromal cells in testis, ovary, and thyroid, whereas the kidney proximal tubule cells, cholangiocytes, and enterocytes are less likely to be the primary SARS-CoV-2 targets. Actually, the stomach may constitute a physical barrier against SARS-CoV-2 as the acidic environment (pH < 2.0) could completely inactivate SARS-CoV-2 pseudo-viruses. Together, we provide a comprehensive view on the potential SARS-CoV-2 targets by pscRNA profiling.http://www.sciencedirect.com/science/article/pii/S258900422030941XVirologyMicrobiologyTranscriptomics |
| spellingShingle | Lulin Zhou Zubiao Niu Xiaoyi Jiang Zhengrong Zhang You Zheng Zhongyi Wang Yichao Zhu Lihua Gao Hongyan Huang Xiaoning Wang Qiang Sun SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases iScience Virology Microbiology Transcriptomics |
| title | SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases |
| title_full | SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases |
| title_fullStr | SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases |
| title_full_unstemmed | SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases |
| title_short | SARS-CoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases |
| title_sort | sars cov 2 targets by the pscrna profiling of ace2 tmprss2 and furin proteases |
| topic | Virology Microbiology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S258900422030941X |
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