Proof of concept for multiple nerve transfers to a single target muscle
Surgical nerve transfers are used to efficiently treat peripheral nerve injuries, neuromas, phantom limb pain, or improve bionic prosthetic control. Commonly, one donor nerve is transferred to one target muscle. However, the transfer of multiple nerves onto a single target muscle may increase the nu...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/71312 |
| _version_ | 1828374600366424064 |
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| author | Matthias Luft Johanna Klepetko Silvia Muceli Jaime Ibáñez Vlad Tereshenko Christopher Festin Gregor Laengle Olga Politikou Udo Maierhofer Dario Farina Oskar C Aszmann Konstantin Davide Bergmeister |
| author_facet | Matthias Luft Johanna Klepetko Silvia Muceli Jaime Ibáñez Vlad Tereshenko Christopher Festin Gregor Laengle Olga Politikou Udo Maierhofer Dario Farina Oskar C Aszmann Konstantin Davide Bergmeister |
| author_sort | Matthias Luft |
| collection | DOAJ |
| description | Surgical nerve transfers are used to efficiently treat peripheral nerve injuries, neuromas, phantom limb pain, or improve bionic prosthetic control. Commonly, one donor nerve is transferred to one target muscle. However, the transfer of multiple nerves onto a single target muscle may increase the number of muscle signals for myoelectric prosthetic control and facilitate the treatment of multiple neuromas. Currently, no experimental models are available. This study describes a novel experimental model to investigate the neurophysiological effects of peripheral double nerve transfers to a common target muscle. In 62 male Sprague-Dawley rats, the ulnar nerve of the antebrachium alone (n=30) or together with the anterior interosseus nerve (n=32) was transferred to reinnervate the long head of the biceps brachii. Before neurotization, the motor branch to the biceps’ long head was transected at the motor entry point. Twelve weeks after surgery, muscle response to neurotomy, behavioral testing, retrograde labeling, and structural analyses were performed to assess reinnervation. These analyses indicated that all nerves successfully reinnervated the target muscle. No aberrant reinnervation was observed by the originally innervating nerve. Our observations suggest a minimal burden for the animal with no signs of functional deficit in daily activities or auto-mutilation in both procedures. Furthermore, standard neurophysiological analyses for nerve and muscle regeneration were applicable. This newly developed nerve transfer model allows for the reliable and standardized investigation of neural and functional changes following the transfer of multiple donor nerves to one target muscle. |
| first_indexed | 2024-04-14T07:35:21Z |
| format | Article |
| id | doaj.art-e5cbe11e856042109b3af1ec33341bb9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:35:21Z |
| publishDate | 2021-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e5cbe11e856042109b3af1ec33341bb92022-12-22T02:05:43ZengeLife Sciences Publications LtdeLife2050-084X2021-10-011010.7554/eLife.71312Proof of concept for multiple nerve transfers to a single target muscleMatthias Luft0https://orcid.org/0000-0002-9161-4125Johanna Klepetko1Silvia Muceli2https://orcid.org/0000-0002-0310-1021Jaime Ibáñez3Vlad Tereshenko4https://orcid.org/0000-0001-7761-5191Christopher Festin5Gregor Laengle6https://orcid.org/0000-0003-1011-3482Olga Politikou7Udo Maierhofer8Dario Farina9https://orcid.org/0000-0002-7883-2697Oskar C Aszmann10Konstantin Davide Bergmeister11https://orcid.org/0000-0003-3910-9727Clinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaDepartment of Electrical Engineering, Chalmers University of Technology, Gothenburg, SwedenDepartment of Bioengineering, Imperial College London, London, United Kingdom; Department of Clinical and Movement Neuroscience, University College London, London, London, United Kingdom; BSICoS Group, IIS Aragón, Universidad de Zaragoza, Zaragoza, SpainClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, AustriaDepartment of Bioengineering, Imperial College London, London, United Kingdom; Department of Clinical and Movement Neuroscience, University College London, London, London, United KingdomClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, AustriaClinical Laboratory for Bionic Extremity Reconstruction, Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna, Austria; Center for Biomedical Research, Medical University of Vienna, Vienna, Austria; Karl Landsteiner University of Health Sciences, Department of Plastic, Aesthetic and ReconstructiveSurgery, University Hospital St. Poelten, St. Poelten, AustriaSurgical nerve transfers are used to efficiently treat peripheral nerve injuries, neuromas, phantom limb pain, or improve bionic prosthetic control. Commonly, one donor nerve is transferred to one target muscle. However, the transfer of multiple nerves onto a single target muscle may increase the number of muscle signals for myoelectric prosthetic control and facilitate the treatment of multiple neuromas. Currently, no experimental models are available. This study describes a novel experimental model to investigate the neurophysiological effects of peripheral double nerve transfers to a common target muscle. In 62 male Sprague-Dawley rats, the ulnar nerve of the antebrachium alone (n=30) or together with the anterior interosseus nerve (n=32) was transferred to reinnervate the long head of the biceps brachii. Before neurotization, the motor branch to the biceps’ long head was transected at the motor entry point. Twelve weeks after surgery, muscle response to neurotomy, behavioral testing, retrograde labeling, and structural analyses were performed to assess reinnervation. These analyses indicated that all nerves successfully reinnervated the target muscle. No aberrant reinnervation was observed by the originally innervating nerve. Our observations suggest a minimal burden for the animal with no signs of functional deficit in daily activities or auto-mutilation in both procedures. Furthermore, standard neurophysiological analyses for nerve and muscle regeneration were applicable. This newly developed nerve transfer model allows for the reliable and standardized investigation of neural and functional changes following the transfer of multiple donor nerves to one target muscle.https://elifesciences.org/articles/71312nerve injuryperipheral nervenerve transfertargeted muscle reinnervationrat modelmotor unit |
| spellingShingle | Matthias Luft Johanna Klepetko Silvia Muceli Jaime Ibáñez Vlad Tereshenko Christopher Festin Gregor Laengle Olga Politikou Udo Maierhofer Dario Farina Oskar C Aszmann Konstantin Davide Bergmeister Proof of concept for multiple nerve transfers to a single target muscle eLife nerve injury peripheral nerve nerve transfer targeted muscle reinnervation rat model motor unit |
| title | Proof of concept for multiple nerve transfers to a single target muscle |
| title_full | Proof of concept for multiple nerve transfers to a single target muscle |
| title_fullStr | Proof of concept for multiple nerve transfers to a single target muscle |
| title_full_unstemmed | Proof of concept for multiple nerve transfers to a single target muscle |
| title_short | Proof of concept for multiple nerve transfers to a single target muscle |
| title_sort | proof of concept for multiple nerve transfers to a single target muscle |
| topic | nerve injury peripheral nerve nerve transfer targeted muscle reinnervation rat model motor unit |
| url | https://elifesciences.org/articles/71312 |
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