Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction
Abstract This study evaluates the effects of a green tea (Camellia sinensis) and hyaluronic acid gel on fibroblast activity and alveolar bone repair following third molar extractions. By examining the gene expression related to cell survival, proliferation, and angiogenesis, the study bridges in vit...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57821-5 |
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| author | Mariana da Silva Bonatto Geórgia da Silva Feltran Thamires Prazeres Barbosa Davisson Alves Pereira Samara de Souza Santos Pedro Gomes Junqueira Mendes Roberto Sales e Pessoa Fábio José Barbosa Bezerra Willian Fernando Zambuzzi Guilherme José Pimentel Lopes de Oliveira |
| author_facet | Mariana da Silva Bonatto Geórgia da Silva Feltran Thamires Prazeres Barbosa Davisson Alves Pereira Samara de Souza Santos Pedro Gomes Junqueira Mendes Roberto Sales e Pessoa Fábio José Barbosa Bezerra Willian Fernando Zambuzzi Guilherme José Pimentel Lopes de Oliveira |
| author_sort | Mariana da Silva Bonatto |
| collection | DOAJ |
| description | Abstract This study evaluates the effects of a green tea (Camellia sinensis) and hyaluronic acid gel on fibroblast activity and alveolar bone repair following third molar extractions. By examining the gene expression related to cell survival, proliferation, and angiogenesis, the study bridges in vitro findings with clinical outcomes in a split-mouth randomized trial. Human fibroblasts were exposed to the treatment gel, analysing gene expression through RT-qPCR. Twenty participants undergoing bilateral third molar extractions received the test gel on one side and a placebo on the other. Assessments included patient-reported outcomes, professional evaluations, and radiographic analyses at multiple postoperative intervals. The test gel significantly enhanced AKT, CDKs, and VEGF gene expressions, indicating a positive effect on angiogenesis and cell proliferation. Clinically, it resulted in reduced exudate, swelling, and secondary interventions, with radiographs showing improved alveolar bone density after 90 days. The green tea and hyaluronic acid gel significantly improves soft tissue and bone healing post-extraction, offering a promising adjunctive therapy for enhancing postoperative recovery. This gel represents a novel adjuvant treatment option for facilitating improved healing outcomes after third molar extractions, highlighting its potential utility in clinical dental practice. |
| first_indexed | 2024-04-24T16:18:09Z |
| format | Article |
| id | doaj.art-e5cf31d3cd334d5fafdffc00de4e4279 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T16:18:09Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e5cf31d3cd334d5fafdffc00de4e42792024-03-31T11:21:35ZengNature PortfolioScientific Reports2045-23222024-03-0114111410.1038/s41598-024-57821-5Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extractionMariana da Silva Bonatto0Geórgia da Silva Feltran1Thamires Prazeres Barbosa2Davisson Alves Pereira3Samara de Souza Santos4Pedro Gomes Junqueira Mendes5Roberto Sales e Pessoa6Fábio José Barbosa Bezerra7Willian Fernando Zambuzzi8Guilherme José Pimentel Lopes de Oliveira9School of Dentistry - Universidade Federal de Uberlândia (UFU)UNESP - Campus de Botucatu - Instituto de Biociências, São Paulo State UniversityUNESP - Campus de Botucatu - Instituto de Biociências, São Paulo State UniversitySchool of Dentistry - Universidade Federal de Uberlândia (UFU)School of Dentistry - Universidade Federal de Uberlândia (UFU)School of Dentistry - Universidade Federal de Uberlândia (UFU)Centro Universitário Do TriânguloUNESP - Campus de Botucatu - Instituto de Biociências, São Paulo State UniversityUNESP - Campus de Botucatu - Instituto de Biociências, São Paulo State UniversitySchool of Dentistry - Universidade Federal de Uberlândia (UFU)Abstract This study evaluates the effects of a green tea (Camellia sinensis) and hyaluronic acid gel on fibroblast activity and alveolar bone repair following third molar extractions. By examining the gene expression related to cell survival, proliferation, and angiogenesis, the study bridges in vitro findings with clinical outcomes in a split-mouth randomized trial. Human fibroblasts were exposed to the treatment gel, analysing gene expression through RT-qPCR. Twenty participants undergoing bilateral third molar extractions received the test gel on one side and a placebo on the other. Assessments included patient-reported outcomes, professional evaluations, and radiographic analyses at multiple postoperative intervals. The test gel significantly enhanced AKT, CDKs, and VEGF gene expressions, indicating a positive effect on angiogenesis and cell proliferation. Clinically, it resulted in reduced exudate, swelling, and secondary interventions, with radiographs showing improved alveolar bone density after 90 days. The green tea and hyaluronic acid gel significantly improves soft tissue and bone healing post-extraction, offering a promising adjunctive therapy for enhancing postoperative recovery. This gel represents a novel adjuvant treatment option for facilitating improved healing outcomes after third molar extractions, highlighting its potential utility in clinical dental practice.https://doi.org/10.1038/s41598-024-57821-5Tissue regenerationBone regenerationOral SurgeryWound healingFibroblastsGreen tea |
| spellingShingle | Mariana da Silva Bonatto Geórgia da Silva Feltran Thamires Prazeres Barbosa Davisson Alves Pereira Samara de Souza Santos Pedro Gomes Junqueira Mendes Roberto Sales e Pessoa Fábio José Barbosa Bezerra Willian Fernando Zambuzzi Guilherme José Pimentel Lopes de Oliveira Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction Scientific Reports Tissue regeneration Bone regeneration Oral Surgery Wound healing Fibroblasts Green tea |
| title | Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction |
| title_full | Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction |
| title_fullStr | Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction |
| title_full_unstemmed | Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction |
| title_short | Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction |
| title_sort | green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post third molar extraction |
| topic | Tissue regeneration Bone regeneration Oral Surgery Wound healing Fibroblasts Green tea |
| url | https://doi.org/10.1038/s41598-024-57821-5 |
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