Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium
Background: Antibacterial and biofilm removal effects of agar hydrogel incorporating silver nanoparticles (SNP) at various concentrations were studied against Staphylococcus aureus and Salmonella typhimurium in vitro. Methods: The minimum inhibitory concentrations (MIC) of SNP was determine...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-10-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | https://jmb.tums.ac.ir/index.php/jmb/article/view/334 |
| _version_ | 1818021080795709440 |
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| author | Leyla Sadat Bouryabaf Mehran Moradi Hossein Tajik Armen Badali |
| author_facet | Leyla Sadat Bouryabaf Mehran Moradi Hossein Tajik Armen Badali |
| author_sort | Leyla Sadat Bouryabaf |
| collection | DOAJ |
| description | Background: Antibacterial and biofilm removal effects of agar hydrogel incorporating silver nanoparticles (SNP) at various concentrations were studied against Staphylococcus aureus and Salmonella typhimurium in vitro.
Methods: The minimum inhibitory concentrations (MIC) of SNP was determined by agar dilution method. Then, hydrogels were prepared by mixing of 0.5% w/v agar and SNP (1/2 MIC, MIC, and 2 MIC) and their inhibitory efficacies against planktonic and biofilm forms of bacteria were measured using agar spot and microtiter test, respectively.
Results: The MIC value was 125 µg/ mL for both bacteria. All SNP hydrogels represented antibacterial activity against Staphylococcus aureus and S. typhimurium on agar culture, which was significant compared to control group (silver sulfadiazine cream). The developed biofilm of S. aureus and S. typhimurium were strongly (85% reduction) and modernly affected (60% reduction) by SNP hydrogels during 15 min contact time, respectively. A dose-dependent biofilm reduction was not demonstrated when different SNP concentrations were tested. Moreover, the results from this study confirmed the moderate sanitizing ability of SNP loaded hydrogel against planktonic forms of both bacteria, which SNP (2MIC) hydrogel decreased only 2.3 log10 CFU/ mL in a primary population of S. typhimurium during 15 min exposure time.
Conclusion: We recommended SNP incorporated agar hydrogel as an effective biofilm removal sanitizer. |
| first_indexed | 2024-04-14T08:14:08Z |
| format | Article |
| id | doaj.art-e5d9bff1c1344ecdb19069aab862f921 |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-04-14T08:14:08Z |
| publishDate | 2017-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-e5d9bff1c1344ecdb19069aab862f9212022-12-22T02:04:28ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812017-10-0163-4290Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimuriumLeyla Sadat Bouryabaf0Mehran Moradi1Hossein Tajik2Armen Badali3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, West Azarbaijan, Iran.Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, West Azarbaijan, Iran.Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, West Azarbaijan, Iran.Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, West Azarbaijan, Iran.Background: Antibacterial and biofilm removal effects of agar hydrogel incorporating silver nanoparticles (SNP) at various concentrations were studied against Staphylococcus aureus and Salmonella typhimurium in vitro. Methods: The minimum inhibitory concentrations (MIC) of SNP was determined by agar dilution method. Then, hydrogels were prepared by mixing of 0.5% w/v agar and SNP (1/2 MIC, MIC, and 2 MIC) and their inhibitory efficacies against planktonic and biofilm forms of bacteria were measured using agar spot and microtiter test, respectively. Results: The MIC value was 125 µg/ mL for both bacteria. All SNP hydrogels represented antibacterial activity against Staphylococcus aureus and S. typhimurium on agar culture, which was significant compared to control group (silver sulfadiazine cream). The developed biofilm of S. aureus and S. typhimurium were strongly (85% reduction) and modernly affected (60% reduction) by SNP hydrogels during 15 min contact time, respectively. A dose-dependent biofilm reduction was not demonstrated when different SNP concentrations were tested. Moreover, the results from this study confirmed the moderate sanitizing ability of SNP loaded hydrogel against planktonic forms of both bacteria, which SNP (2MIC) hydrogel decreased only 2.3 log10 CFU/ mL in a primary population of S. typhimurium during 15 min exposure time. Conclusion: We recommended SNP incorporated agar hydrogel as an effective biofilm removal sanitizer.https://jmb.tums.ac.ir/index.php/jmb/article/view/334AgarAntibacterialBiofilmHydrogelNanoparticlesSanitizing |
| spellingShingle | Leyla Sadat Bouryabaf Mehran Moradi Hossein Tajik Armen Badali Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium Journal of Medical Bacteriology Agar Antibacterial Biofilm Hydrogel Nanoparticles Sanitizing |
| title | Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium |
| title_full | Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium |
| title_fullStr | Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium |
| title_full_unstemmed | Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium |
| title_short | Biofilm Removal and Antimicrobial Activities of Agar Hydrogel Containing Colloid Nano-Silver against Staphylococcus aureus and Salmonella typhimurium |
| title_sort | biofilm removal and antimicrobial activities of agar hydrogel containing colloid nano silver against staphylococcus aureus and salmonella typhimurium |
| topic | Agar Antibacterial Biofilm Hydrogel Nanoparticles Sanitizing |
| url | https://jmb.tums.ac.ir/index.php/jmb/article/view/334 |
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