Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings
Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phlo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.948171/full |
| _version_ | 1811341688491212800 |
|---|---|
| author | Hongzhen Zhang Chenghua Zhang Xiaolong Xiang Xiaolong Xiang Qilun Zhang Wei Zhao Guoyu Wei Anlong Hu |
| author_facet | Hongzhen Zhang Chenghua Zhang Xiaolong Xiang Xiaolong Xiang Qilun Zhang Wei Zhao Guoyu Wei Anlong Hu |
| author_sort | Hongzhen Zhang |
| collection | DOAJ |
| description | Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phloem transport, was used to study the mechanism of uptake and transport of KSM. Results showed that cotyledon-applied KSM could transport into the phloem and distributed in root and shoot of plant. The temperature, concentration, and pH had significant effects on the uptake of KSM, indicating that the uptake of KSM was mediated by an active carrier system. Compared with the control, competitive inhibitors of sugar transporters D-glucose, D-chiro-inositol, and phloridzin inhibited 71.03%, 67.95%, and 61.73% uptake of KSM, respectively. Energy inhibitor dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also affected the uptake of KSM, and the inhibition rates were 34.23% and 48.06%. All the results showed that the uptake of KSM was mediated by a sugar transporter, and it could transport from shoot to root in plants via the phloem. The study preliminary elucidated the plant–microbe interactions in the context of the transport of microbial secondary metabolites in plants. It has certain significance for scientific application of antibiotics and biological control of plant diseases and provides theoretical basis for the development of bidirectional transport pesticides. |
| first_indexed | 2024-04-13T18:58:36Z |
| format | Article |
| id | doaj.art-e5dc7ad20d0d4bdab3282b1224ee2abd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T18:58:36Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e5dc7ad20d0d4bdab3282b1224ee2abd2022-12-22T02:34:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.948171948171Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlingsHongzhen Zhang0Chenghua Zhang1Xiaolong Xiang2Xiaolong Xiang3Qilun Zhang4Wei Zhao5Guoyu Wei6Anlong Hu7College of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaShandong Academy of Agricultural Sciences, Jinan, Shandong, ChinaCollege of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaForestry Bureau of Wuchuan County, Zunyi, Guizhou, ChinaCollege of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaCollege of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaCollege of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaCollege of Agriculture, Guizhou University, Guiyang, Guizhou, ChinaKasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phloem transport, was used to study the mechanism of uptake and transport of KSM. Results showed that cotyledon-applied KSM could transport into the phloem and distributed in root and shoot of plant. The temperature, concentration, and pH had significant effects on the uptake of KSM, indicating that the uptake of KSM was mediated by an active carrier system. Compared with the control, competitive inhibitors of sugar transporters D-glucose, D-chiro-inositol, and phloridzin inhibited 71.03%, 67.95%, and 61.73% uptake of KSM, respectively. Energy inhibitor dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also affected the uptake of KSM, and the inhibition rates were 34.23% and 48.06%. All the results showed that the uptake of KSM was mediated by a sugar transporter, and it could transport from shoot to root in plants via the phloem. The study preliminary elucidated the plant–microbe interactions in the context of the transport of microbial secondary metabolites in plants. It has certain significance for scientific application of antibiotics and biological control of plant diseases and provides theoretical basis for the development of bidirectional transport pesticides.https://www.frontiersin.org/articles/10.3389/fmicb.2022.948171/fullkasugamycinantibioticuptakeplanttransport |
| spellingShingle | Hongzhen Zhang Chenghua Zhang Xiaolong Xiang Xiaolong Xiang Qilun Zhang Wei Zhao Guoyu Wei Anlong Hu Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings Frontiers in Microbiology kasugamycin antibiotic uptake plant transport |
| title | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
| title_full | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
| title_fullStr | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
| title_full_unstemmed | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
| title_short | Uptake and transport of antibiotic kasugamycin in castor bean (Ricinus communis L.) seedlings |
| title_sort | uptake and transport of antibiotic kasugamycin in castor bean ricinus communis l seedlings |
| topic | kasugamycin antibiotic uptake plant transport |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.948171/full |
| work_keys_str_mv | AT hongzhenzhang uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT chenghuazhang uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT xiaolongxiang uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT xiaolongxiang uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT qilunzhang uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT weizhao uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT guoyuwei uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings AT anlonghu uptakeandtransportofantibiotickasugamycinincastorbeanricinuscommunislseedlings |