Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant
Stress-associated proteins (SAP), a class of zinc-finger proteins, have been identified as novel stress regulatory proteins in stress responses. However, <i>SAP</i> genes in eggplant (<i>SmSAP</i>) have been little reported. It has important significance in identifying SAP me...
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MDPI AG
2022-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/2/108 |
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| author | Faxiang Wan Yuhu Xu Sulong Wang Jun Gao Dan Lu Chenghong Zhou Yanqing Liao Yanyan Ma Yu Zheng |
| author_facet | Faxiang Wan Yuhu Xu Sulong Wang Jun Gao Dan Lu Chenghong Zhou Yanqing Liao Yanyan Ma Yu Zheng |
| author_sort | Faxiang Wan |
| collection | DOAJ |
| description | Stress-associated proteins (SAP), a class of zinc-finger proteins, have been identified as novel stress regulatory proteins in stress responses. However, <i>SAP</i> genes in eggplant (<i>SmSAP</i>) have been little reported. It has important significance in identifying SAP members, understanding the molecular mechanisms underlying stress responses, and tolerance. We performed a comprehensive study of the A20/AN1 domains, motifs, gene structures, phylogenetic relationships, chromosomal locations, gene replications, collinearity, cis-acting elements, and expression pattern responses to various abiotic stresses. Twenty-one <i>SAP</i> genes were identified in eggplant (<i>SmSAP</i>) and were localized on 10 chromosomes. A phylogenetic analysis revealed that most of the SmSAP proteins showed a high homology with the tomato SAP members, and 21 members were divided into four groups based on the homology of the SAP members in eggplant, tomato, rice, and <i>Arabidopsis</i>. Further analysis revealed that SmSAP proteins contain the characteristic A20/AN1 domains, the A20 domain composed of motif 2 (ILCINNCGFFGSPATMNLCSKCYKDMJLK). Four pairs of tandem duplications were found in eggplant, and 10 <i>SmSAP</i> genes had collinearity with <i>SAP</i> genes from <i>Arabidopsis</i>, potato, or tomato, but only four <i>SmSAP</i> genes were collinear with <i>SAP</i> genes in the three species mentioned above. Moreover, the promoters of <i>SmSAP</i> genes were predicted to contain many cis-acting elements that respond to abiotic stress and hormones. A qRT-PCR analysis of the four selected <i>SmSAP</i> genes exhibited diverse expression levels in response to various environmental stresses. These results provided a comprehensive analysis of the <i>SmSAP</i> genes and lay a solid foundation for improving the understanding of the functional diversification of <i>SAP</i> genes under various environmental stresses in eggplant. |
| first_indexed | 2024-03-09T21:48:28Z |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T21:48:28Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-e5de85a7d9364e77953926900f78654a2023-11-23T20:11:48ZengMDPI AGHorticulturae2311-75242022-01-018210810.3390/horticulturae8020108Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in EggplantFaxiang Wan0Yuhu Xu1Sulong Wang2Jun Gao3Dan Lu4Chenghong Zhou5Yanqing Liao6Yanyan Ma7Yu Zheng8Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaFaculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, ChinaCitrus Research Institute, Southwest University, Chongqing 400712, ChinaChongqing Vocational Institute of Engineering, Chongqing 402260, ChinaStress-associated proteins (SAP), a class of zinc-finger proteins, have been identified as novel stress regulatory proteins in stress responses. However, <i>SAP</i> genes in eggplant (<i>SmSAP</i>) have been little reported. It has important significance in identifying SAP members, understanding the molecular mechanisms underlying stress responses, and tolerance. We performed a comprehensive study of the A20/AN1 domains, motifs, gene structures, phylogenetic relationships, chromosomal locations, gene replications, collinearity, cis-acting elements, and expression pattern responses to various abiotic stresses. Twenty-one <i>SAP</i> genes were identified in eggplant (<i>SmSAP</i>) and were localized on 10 chromosomes. A phylogenetic analysis revealed that most of the SmSAP proteins showed a high homology with the tomato SAP members, and 21 members were divided into four groups based on the homology of the SAP members in eggplant, tomato, rice, and <i>Arabidopsis</i>. Further analysis revealed that SmSAP proteins contain the characteristic A20/AN1 domains, the A20 domain composed of motif 2 (ILCINNCGFFGSPATMNLCSKCYKDMJLK). Four pairs of tandem duplications were found in eggplant, and 10 <i>SmSAP</i> genes had collinearity with <i>SAP</i> genes from <i>Arabidopsis</i>, potato, or tomato, but only four <i>SmSAP</i> genes were collinear with <i>SAP</i> genes in the three species mentioned above. Moreover, the promoters of <i>SmSAP</i> genes were predicted to contain many cis-acting elements that respond to abiotic stress and hormones. A qRT-PCR analysis of the four selected <i>SmSAP</i> genes exhibited diverse expression levels in response to various environmental stresses. These results provided a comprehensive analysis of the <i>SmSAP</i> genes and lay a solid foundation for improving the understanding of the functional diversification of <i>SAP</i> genes under various environmental stresses in eggplant.https://www.mdpi.com/2311-7524/8/2/108A20/AN1 domainstress-associated genesexpression analysisabiotic stresseggplant |
| spellingShingle | Faxiang Wan Yuhu Xu Sulong Wang Jun Gao Dan Lu Chenghong Zhou Yanqing Liao Yanyan Ma Yu Zheng Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant Horticulturae A20/AN1 domain stress-associated genes expression analysis abiotic stress eggplant |
| title | Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant |
| title_full | Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant |
| title_fullStr | Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant |
| title_full_unstemmed | Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant |
| title_short | Identification and Expression Analysis of Zinc Finger A20/AN1 Stress-Associated Genes SmSAP Responding to Abiotic Stress in Eggplant |
| title_sort | identification and expression analysis of zinc finger a20 an1 stress associated genes smsap responding to abiotic stress in eggplant |
| topic | A20/AN1 domain stress-associated genes expression analysis abiotic stress eggplant |
| url | https://www.mdpi.com/2311-7524/8/2/108 |
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