The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)
This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (m...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2019-03-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201901-0004_the-effect-of-magnetic-field-strength-on-shoot-regeneration-and-agrobacterium-tumefaciens-mediated-gene-transfe.php |
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| author | Murat Aycan Ramazan Beyaz Anzel Bahadir Mustafa Yildiz |
| author_facet | Murat Aycan Ramazan Beyaz Anzel Bahadir Mustafa Yildiz |
| author_sort | Murat Aycan |
| collection | DOAJ |
| description | This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength. |
| first_indexed | 2024-04-10T08:28:54Z |
| format | Article |
| id | doaj.art-e5dfbebe4d18480387661063a1478876 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:28:54Z |
| publishDate | 2019-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-e5dfbebe4d18480387661063a14788762023-02-23T03:30:46ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252019-03-01551202710.17221/195/2017-CJGPBcjg-201901-0004The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)Murat Aycan0Ramazan Beyaz1Anzel Bahadir2Mustafa Yildiz3Department of Field Crops, Graduate School of Natural and Applied Sciences, Ankara University, Diskapi, Ankara, TurkeyDepartment of Soil and Plant Nutrition, Faculty of Agriculture, Ahi Evran University, Bağbaşi, Kirşehir, TurkeyDepartment of Biophysics, Faculty of Medicine, Duzce University, Konuralp, Duzce, TurkeyDepartment of Field Crops, Faculty of Agriculture, Ankara University, Diskapi, Ankara, TurkeyThis study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.https://cjgpb.agriculturejournals.cz/artkey/cjg-201901-0004_the-effect-of-magnetic-field-strength-on-shoot-regeneration-and-agrobacterium-tumefaciens-mediated-gene-transfe.phpagrobacterium tumefacienslinum usitatissimummagnetic fieldregeneration efficiencytransformation |
| spellingShingle | Murat Aycan Ramazan Beyaz Anzel Bahadir Mustafa Yildiz The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) Czech Journal of Genetics and Plant Breeding agrobacterium tumefaciens linum usitatissimum magnetic field regeneration efficiency transformation |
| title | The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) |
| title_full | The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) |
| title_fullStr | The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) |
| title_full_unstemmed | The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) |
| title_short | The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) |
| title_sort | effect of magnetic field strength on shoot regeneration and agrobacterium tumefaciens mediated gene transfer in flax linum usitatissimum l |
| topic | agrobacterium tumefaciens linum usitatissimum magnetic field regeneration efficiency transformation |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201901-0004_the-effect-of-magnetic-field-strength-on-shoot-regeneration-and-agrobacterium-tumefaciens-mediated-gene-transfe.php |
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