Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides
The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated. Polysaccharides were treated at 50 °C with hydrochloric acid for 3–48 h. Portions of isopropanol (<i>i</i>-PrOH) were added sequentially to the h...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/13/2982 |
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| author | Cristina Lupo Samy Boulos Laura Nyström |
| author_facet | Cristina Lupo Samy Boulos Laura Nyström |
| author_sort | Cristina Lupo |
| collection | DOAJ |
| description | The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated. Polysaccharides were treated at 50 °C with hydrochloric acid for 3–48 h. Portions of isopropanol (<i>i</i>-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation. As expected, a significant reduction of weight-average molecular weight (<i>M<sub>w</sub></i>) was observed with increasing hydrolysis time. Fractional precipitation was successfully applied to collect at least one polymer fraction with dispersity (<i>Đ</i>) close to one for each polysaccharide. The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected. Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (<i>α</i>), confirmed that acid hydrolysis influenced the polysaccharides’ conformation. It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation. |
| first_indexed | 2024-03-10T18:48:52Z |
| format | Article |
| id | doaj.art-e5e26524b2dd400ca39367140f4114de |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:48:52Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e5e26524b2dd400ca39367140f4114de2023-11-20T05:19:36ZengMDPI AGMolecules1420-30492020-06-012513298210.3390/molecules25132982Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble PolysaccharidesCristina Lupo0Samy Boulos1Laura Nyström2Department of Health Science and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, SwitzerlandDepartment of Health Science and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, SwitzerlandDepartment of Health Science and Technology, Institute of Food, Nutrition and Health, ETH Zurich, 8092 Zurich, SwitzerlandThe effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated. Polysaccharides were treated at 50 °C with hydrochloric acid for 3–48 h. Portions of isopropanol (<i>i</i>-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation. As expected, a significant reduction of weight-average molecular weight (<i>M<sub>w</sub></i>) was observed with increasing hydrolysis time. Fractional precipitation was successfully applied to collect at least one polymer fraction with dispersity (<i>Đ</i>) close to one for each polysaccharide. The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected. Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (<i>α</i>), confirmed that acid hydrolysis influenced the polysaccharides’ conformation. It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.https://www.mdpi.com/1420-3049/25/13/2982water-soluble polysaccharidesdietary fiberspartial acid hydrolysisfractional precipitation with isopropanolmonosaccharide ratioMark-Houwink-Sakurada relationship |
| spellingShingle | Cristina Lupo Samy Boulos Laura Nyström Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides Molecules water-soluble polysaccharides dietary fibers partial acid hydrolysis fractional precipitation with isopropanol monosaccharide ratio Mark-Houwink-Sakurada relationship |
| title | Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides |
| title_full | Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides |
| title_fullStr | Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides |
| title_full_unstemmed | Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides |
| title_short | Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides |
| title_sort | influence of partial acid hydrolysis on size dispersity monosaccharide composition and conformation of linearly branched water soluble polysaccharides |
| topic | water-soluble polysaccharides dietary fibers partial acid hydrolysis fractional precipitation with isopropanol monosaccharide ratio Mark-Houwink-Sakurada relationship |
| url | https://www.mdpi.com/1420-3049/25/13/2982 |
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