Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR

Sensitive and Rapid Detection of Traditional Chinese Herbs by Loop-Mediated Isothermal Amplification Method and Real-Time Fluorescence Quantitative PCR

ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and...

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Bibliographic Details
Main Authors: Min Min Ma, Dan Jiang, Shuang Ya Li, Yuan Wu, Ju Meng, Jiang Yao Huang
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2018-11-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Traditional Chinese medicine
Real-time PCR
Phellodendron chinense Schneid
LAMP
Specificity
Sensitivity
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314&lng=en&tlng=en

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