Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon
Zika virus (ZIKV) is an RNA flavivirus (<i>Flaviviridae</i> family) endemic in tropical and subtropical regions that is transmitted to humans by <i>Aedes</i> (<i>Stegomyia</i>) species mosquitoes. The two main urban vectors of ZIKV are <i>Aedes aegypti</i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/6/1356 |
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| author | Erika Oliveira Gomes Lívia Sacchetto Maurício Teixeira Bárbara Aparecida Chaves Adam Hendy Claudia Mendonça Izabele Guimarães Ramon Linhares Daniela Brito Danielle Valério Jady Shayenne Mota Cordeiro Alexandre Vilhena Silva Neto Vanderson Souza Sampaio Vera Margarete Scarpassa Michaela Buenemann Nikos Vasilakis Djane Clarys Baia-da-Silva Maurício Lacerda Nogueira Maria Paula Gomes Mourão Marcus Vinícius Guimarães Lacerda |
| author_facet | Erika Oliveira Gomes Lívia Sacchetto Maurício Teixeira Bárbara Aparecida Chaves Adam Hendy Claudia Mendonça Izabele Guimarães Ramon Linhares Daniela Brito Danielle Valério Jady Shayenne Mota Cordeiro Alexandre Vilhena Silva Neto Vanderson Souza Sampaio Vera Margarete Scarpassa Michaela Buenemann Nikos Vasilakis Djane Clarys Baia-da-Silva Maurício Lacerda Nogueira Maria Paula Gomes Mourão Marcus Vinícius Guimarães Lacerda |
| author_sort | Erika Oliveira Gomes |
| collection | DOAJ |
| description | Zika virus (ZIKV) is an RNA flavivirus (<i>Flaviviridae</i> family) endemic in tropical and subtropical regions that is transmitted to humans by <i>Aedes</i> (<i>Stegomyia</i>) species mosquitoes. The two main urban vectors of ZIKV are <i>Aedes aegypti</i> and <i>Aedes albopictus,</i> which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female <i>Ae. aegypti</i> (22 specimens) and <i>Ae. albopictus</i> (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) <i>Ae. aegypti</i> and 5/241 (2%) <i>Ae. albopictus</i> pools screened using RT-qPCR were positive for ZIKV. No supernatants from <i>Ae. aegypti</i> were positive for ZIKV (0%), and 15 out of 241 (6.2%) <i>Ae. albopictus</i> pools were positive. In this study, we provide the first-ever evidence of <i>Ae. albopictus</i> naturally infected with ZIKV in the Amazon region. |
| first_indexed | 2024-03-11T01:50:31Z |
| format | Article |
| id | doaj.art-e5e76fa4c090441e8dc062049a240c64 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:50:31Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e5e76fa4c090441e8dc062049a240c642023-11-18T13:02:40ZengMDPI AGViruses1999-49152023-06-01156135610.3390/v15061356Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian AmazonErika Oliveira Gomes0Lívia Sacchetto1Maurício Teixeira2Bárbara Aparecida Chaves3Adam Hendy4Claudia Mendonça5Izabele Guimarães6Ramon Linhares7Daniela Brito8Danielle Valério9Jady Shayenne Mota Cordeiro10Alexandre Vilhena Silva Neto11Vanderson Souza Sampaio12Vera Margarete Scarpassa13Michaela Buenemann14Nikos Vasilakis15Djane Clarys Baia-da-Silva16Maurício Lacerda Nogueira17Maria Paula Gomes Mourão18Marcus Vinícius Guimarães Lacerda19Universidade do Estado do Amazonas (UEA), Manaus 69850-000, AM, BrazilLaboratório de Pesquisa em Virologia, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, SP, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USAPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilPrograma de Pós-Graduação em Medicina Tropical, PPGMT, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, BrazilLaboratório de Genética Populacional e Evolução de Mosquitos Vetores da Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus 69067-375, AM, BrazilDepartment of Geography, New Mexico State University, Las Cruces, NM 88003, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USAUniversidade do Estado do Amazonas (UEA), Manaus 69850-000, AM, BrazilLaboratório de Pesquisa em Virologia, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto 15090-000, SP, BrazilUniversidade do Estado do Amazonas (UEA), Manaus 69850-000, AM, BrazilUniversidade do Estado do Amazonas (UEA), Manaus 69850-000, AM, BrazilZika virus (ZIKV) is an RNA flavivirus (<i>Flaviviridae</i> family) endemic in tropical and subtropical regions that is transmitted to humans by <i>Aedes</i> (<i>Stegomyia</i>) species mosquitoes. The two main urban vectors of ZIKV are <i>Aedes aegypti</i> and <i>Aedes albopictus,</i> which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female <i>Ae. aegypti</i> (22 specimens) and <i>Ae. albopictus</i> (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) <i>Ae. aegypti</i> and 5/241 (2%) <i>Ae. albopictus</i> pools screened using RT-qPCR were positive for ZIKV. No supernatants from <i>Ae. aegypti</i> were positive for ZIKV (0%), and 15 out of 241 (6.2%) <i>Ae. albopictus</i> pools were positive. In this study, we provide the first-ever evidence of <i>Ae. albopictus</i> naturally infected with ZIKV in the Amazon region.https://www.mdpi.com/1999-4915/15/6/1356mosquito surveillancearbovirusforestAmazonZIKV |
| spellingShingle | Erika Oliveira Gomes Lívia Sacchetto Maurício Teixeira Bárbara Aparecida Chaves Adam Hendy Claudia Mendonça Izabele Guimarães Ramon Linhares Daniela Brito Danielle Valério Jady Shayenne Mota Cordeiro Alexandre Vilhena Silva Neto Vanderson Souza Sampaio Vera Margarete Scarpassa Michaela Buenemann Nikos Vasilakis Djane Clarys Baia-da-Silva Maurício Lacerda Nogueira Maria Paula Gomes Mourão Marcus Vinícius Guimarães Lacerda Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon Viruses mosquito surveillance arbovirus forest Amazon ZIKV |
| title | Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon |
| title_full | Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon |
| title_fullStr | Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon |
| title_full_unstemmed | Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon |
| title_short | Detection of Zika Virus in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon |
| title_sort | detection of zika virus in i aedes aegypti i and i aedes albopictus i mosquitoes collected in urban forest fragments in the brazilian amazon |
| topic | mosquito surveillance arbovirus forest Amazon ZIKV |
| url | https://www.mdpi.com/1999-4915/15/6/1356 |
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