Resistance of oxidative stress in biofilm and planktonic cells
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stre...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-04-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300&lng=en&tlng=en |
| _version_ | 1818244152197906432 |
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| author | Witold Jakubowski Bogdan Walkowiak |
| author_facet | Witold Jakubowski Bogdan Walkowiak |
| author_sort | Witold Jakubowski |
| collection | DOAJ |
| description | This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in logarithmic phase of growth treated with H2O2, both in planktonic and biofilm forms, whereas for the cells in stationary phase, the highest resistance was observed for menadione. These results showed higher efficiency of agents based on superoxide anion donors in combating bacteria colonizing abiotic surfaces stainless steel (AISI 316L). |
| first_indexed | 2024-12-12T14:12:28Z |
| format | Article |
| id | doaj.art-e5eb5a537520400e9f9b225aecec937f |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-12T14:12:28Z |
| publishDate | 2015-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-e5eb5a537520400e9f9b225aecec937f2022-12-22T00:22:04ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-04-0158230030810.1590/S1516-8913201500356S1516-89132015000200300Resistance of oxidative stress in biofilm and planktonic cellsWitold JakubowskiBogdan WalkowiakThis work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in logarithmic phase of growth treated with H2O2, both in planktonic and biofilm forms, whereas for the cells in stationary phase, the highest resistance was observed for menadione. These results showed higher efficiency of agents based on superoxide anion donors in combating bacteria colonizing abiotic surfaces stainless steel (AISI 316L).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300&lng=en&tlng=enreactive oxygen speciesbiofilmbacterial colonizationplanktonic cellsimplant |
| spellingShingle | Witold Jakubowski Bogdan Walkowiak Resistance of oxidative stress in biofilm and planktonic cells Brazilian Archives of Biology and Technology reactive oxygen species biofilm bacterial colonization planktonic cells implant |
| title | Resistance of oxidative stress in biofilm and planktonic cells |
| title_full | Resistance of oxidative stress in biofilm and planktonic cells |
| title_fullStr | Resistance of oxidative stress in biofilm and planktonic cells |
| title_full_unstemmed | Resistance of oxidative stress in biofilm and planktonic cells |
| title_short | Resistance of oxidative stress in biofilm and planktonic cells |
| title_sort | resistance of oxidative stress in biofilm and planktonic cells |
| topic | reactive oxygen species biofilm bacterial colonization planktonic cells implant |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300&lng=en&tlng=en |
| work_keys_str_mv | AT witoldjakubowski resistanceofoxidativestressinbiofilmandplanktoniccells AT bogdanwalkowiak resistanceofoxidativestressinbiofilmandplanktoniccells |