Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level
To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02798-4 |
| _version_ | 1819006338262368256 |
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| author | Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren |
| author_facet | Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren |
| author_sort | Peter Lönn |
| collection | DOAJ |
| description | To improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput. |
| first_indexed | 2024-12-21T00:07:06Z |
| format | Article |
| id | doaj.art-e5ecdbaa06634e1d97b74de9458d189e |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-21T00:07:06Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-e5ecdbaa06634e1d97b74de9458d189e2022-12-21T19:22:27ZengNature PortfolioCommunications Biology2399-36422021-11-01411910.1038/s42003-021-02798-4Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell levelPeter Lönn0Rasel A. Al-Amin1Ehsan Manouchehri Doulabi2Johan Heldin3Radiosa Gallini4Johan Björkesten5Johan Oelrich6Masood Kamali-Moghaddam7Ulf Landegren8Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala UniversityTo improve our ability to monitor cellular responses to e.g. cytokines or drugs, Lönn et al have developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA) in HaCAT keratinocyte cells. Their approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.https://doi.org/10.1038/s42003-021-02798-4 |
| spellingShingle | Peter Lönn Rasel A. Al-Amin Ehsan Manouchehri Doulabi Johan Heldin Radiosa Gallini Johan Björkesten Johan Oelrich Masood Kamali-Moghaddam Ulf Landegren Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level Communications Biology |
| title | Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level |
| title_full | Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level |
| title_fullStr | Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level |
| title_full_unstemmed | Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level |
| title_short | Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level |
| title_sort | image based high throughput mapping of tgf β induced phosphocomplexes at a single cell level |
| url | https://doi.org/10.1038/s42003-021-02798-4 |
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