Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults
Background/purpose: Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features and proteo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790214001408 |
| _version_ | 1828422344458108928 |
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| author | Shan Jiang Xiaojing Huang Chengfei Zhang Zhiyu Cai Ting Zou |
| author_facet | Shan Jiang Xiaojing Huang Chengfei Zhang Zhiyu Cai Ting Zou |
| author_sort | Shan Jiang |
| collection | DOAJ |
| description | Background/purpose: Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features and proteomes of biofilms of S. mutans clinical isolates.
Materials and methods: Clinical strains isolated from caries-active (SM 593) and caries-free (SM 18) adults were cultured on polystyrene sheets in tryptone–polypeptone–yeast extract medium. Biofilm formation and structure were assessed by confocal laser scanning microscopy and scanning electron microscopy. Proteins were extracted from SM 593 and SM 18 presented in biofilms and separated with two-dimensional gel electrophoresis, followed by peptide mass fingerprinting using matrix-assisted laser desorption time-of-flight mass spectrometry analysis.
Results: Initially detected 2 hours after incubation, biofilm formation reached its maximum level at 20 hours. The biofilm formed by SM 593 was thicker with a higher percentage of viable bacteria compared with that formed by SM 18. Hydrolase and pantothenate kinase were detected in the SM 593 biofilm only, whereas 6-pyruvoyl tetrahydropterin synthase and phosphoribosylglycinamide formyltransferase were expressed exclusively in the SM 18 biofilm. Expressions of D-alanyl-D-alanine carboxypeptidase and response regulator homolog of RumR were most greatly enhanced in the SM 593 and SM 18 biofilms, respectively.
Conclusion: SM 593 exhibited greater biofilm-forming capacity compared to SM 18. SM 593 and SM 18 biofilms expressed specific proteins involved in nucleic acid metabolism and intermediary metabolism, respectively, which may account for the differences in their biofilm-forming abilities. |
| first_indexed | 2024-12-10T15:46:12Z |
| format | Article |
| id | doaj.art-e5ee5bf37f1b4db69da9dc76e883194f |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-10T15:46:12Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-e5ee5bf37f1b4db69da9dc76e883194f2022-12-22T01:42:56ZengElsevierJournal of Dental Sciences1991-79022015-06-0110220621510.1016/j.jds.2014.09.001Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adultsShan Jiang0Xiaojing Huang1Chengfei Zhang2Zhiyu Cai3Ting Zou4Department of Endodontics and Operative Dentistry I, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Endodontics and Operative Dentistry I, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, ChinaDepartment of Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, ChinaDepartment of Oral and Maxillofacial Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian, ChinaDepartment of Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong Special Administrative Region, ChinaBackground/purpose: Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features and proteomes of biofilms of S. mutans clinical isolates. Materials and methods: Clinical strains isolated from caries-active (SM 593) and caries-free (SM 18) adults were cultured on polystyrene sheets in tryptone–polypeptone–yeast extract medium. Biofilm formation and structure were assessed by confocal laser scanning microscopy and scanning electron microscopy. Proteins were extracted from SM 593 and SM 18 presented in biofilms and separated with two-dimensional gel electrophoresis, followed by peptide mass fingerprinting using matrix-assisted laser desorption time-of-flight mass spectrometry analysis. Results: Initially detected 2 hours after incubation, biofilm formation reached its maximum level at 20 hours. The biofilm formed by SM 593 was thicker with a higher percentage of viable bacteria compared with that formed by SM 18. Hydrolase and pantothenate kinase were detected in the SM 593 biofilm only, whereas 6-pyruvoyl tetrahydropterin synthase and phosphoribosylglycinamide formyltransferase were expressed exclusively in the SM 18 biofilm. Expressions of D-alanyl-D-alanine carboxypeptidase and response regulator homolog of RumR were most greatly enhanced in the SM 593 and SM 18 biofilms, respectively. Conclusion: SM 593 exhibited greater biofilm-forming capacity compared to SM 18. SM 593 and SM 18 biofilms expressed specific proteins involved in nucleic acid metabolism and intermediary metabolism, respectively, which may account for the differences in their biofilm-forming abilities.http://www.sciencedirect.com/science/article/pii/S1991790214001408biofilmdental caries susceptibilitymorphologyproteomeStreptococcus mutans |
| spellingShingle | Shan Jiang Xiaojing Huang Chengfei Zhang Zhiyu Cai Ting Zou Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults Journal of Dental Sciences biofilm dental caries susceptibility morphology proteome Streptococcus mutans |
| title | Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults |
| title_full | Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults |
| title_fullStr | Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults |
| title_full_unstemmed | Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults |
| title_short | Morphological and proteomic analyses of the biofilms generated by Streptococcus mutans isolated from caries-active and caries-free adults |
| title_sort | morphological and proteomic analyses of the biofilms generated by streptococcus mutans isolated from caries active and caries free adults |
| topic | biofilm dental caries susceptibility morphology proteome Streptococcus mutans |
| url | http://www.sciencedirect.com/science/article/pii/S1991790214001408 |
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