Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential
Concentrated Growth Factors (CGF) represent new autologous (blood-derived biomaterial), attracting growing interest in the field of regenerative medicine. In this study, the chemical, structural, and biological characterization of CGF was carried out. CGF molecular characterization was performed by...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8867 |
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| author | Eleonora Stanca Nadia Calabriso Laura Giannotti Paola Nitti Fabrizio Damiano Benedetta Di Chiara Stanca Maria Annunziata Carluccio Giuseppe Egidio De Benedetto Christian Demitri Andrea Palermo Franco Ferrante Luisa Siculella Alessio Rochira |
| author_facet | Eleonora Stanca Nadia Calabriso Laura Giannotti Paola Nitti Fabrizio Damiano Benedetta Di Chiara Stanca Maria Annunziata Carluccio Giuseppe Egidio De Benedetto Christian Demitri Andrea Palermo Franco Ferrante Luisa Siculella Alessio Rochira |
| author_sort | Eleonora Stanca |
| collection | DOAJ |
| description | Concentrated Growth Factors (CGF) represent new autologous (blood-derived biomaterial), attracting growing interest in the field of regenerative medicine. In this study, the chemical, structural, and biological characterization of CGF was carried out. CGF molecular characterization was performed by GC/MS to quantify small metabolites and by ELISA to measure growth factors and matrix metalloproteinases (MMPs) release; structural CGF characterization was carried out by SEM analysis and immunohistochemistry; CGF has been cultured, and its primary cells were isolated for the identification of their surface markers by flow cytometry, Western blot, and real-time PCR; finally, the osteogenic differentiation of CGF primary cells was evaluated through matrix mineralization by alizarin red staining and through mRNA quantification of osteogenic differentiation markers by real-time PCR. We found that CGF has a complex inner structure capable of influencing the release of growth factors, metabolites, and cells. These cells, which could regulate the production and release of the CGF growth factors, show stem features and are able to differentiate into osteoblasts producing a mineralized matrix. These data, taken together, highlight interesting new perspectives for the use of CGF in regenerative medicine. |
| first_indexed | 2024-03-10T08:44:22Z |
| format | Article |
| id | doaj.art-e5ef3c29e0244d89a7f7b4311909b3d5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:22Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e5ef3c29e0244d89a7f7b4311909b3d52023-11-22T08:01:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216886710.3390/ijms22168867Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic PotentialEleonora Stanca0Nadia Calabriso1Laura Giannotti2Paola Nitti3Fabrizio Damiano4Benedetta Di Chiara Stanca5Maria Annunziata Carluccio6Giuseppe Egidio De Benedetto7Christian Demitri8Andrea Palermo9Franco Ferrante10Luisa Siculella11Alessio Rochira12Laboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyNational Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyNational Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, ItalyAnalytical and Isotopic Mass Spectrometry Laboratory, Department of Cultural Heritage, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, ItalyAssociate Professor in Implant Dentistry College of Medicine and Dentistry, Birmingham B4 6BN, UKSpecialist in Oral Surgery, Private Practioner, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyConcentrated Growth Factors (CGF) represent new autologous (blood-derived biomaterial), attracting growing interest in the field of regenerative medicine. In this study, the chemical, structural, and biological characterization of CGF was carried out. CGF molecular characterization was performed by GC/MS to quantify small metabolites and by ELISA to measure growth factors and matrix metalloproteinases (MMPs) release; structural CGF characterization was carried out by SEM analysis and immunohistochemistry; CGF has been cultured, and its primary cells were isolated for the identification of their surface markers by flow cytometry, Western blot, and real-time PCR; finally, the osteogenic differentiation of CGF primary cells was evaluated through matrix mineralization by alizarin red staining and through mRNA quantification of osteogenic differentiation markers by real-time PCR. We found that CGF has a complex inner structure capable of influencing the release of growth factors, metabolites, and cells. These cells, which could regulate the production and release of the CGF growth factors, show stem features and are able to differentiate into osteoblasts producing a mineralized matrix. These data, taken together, highlight interesting new perspectives for the use of CGF in regenerative medicine.https://www.mdpi.com/1422-0067/22/16/8867CGFgrowth factorstem cellsblood-derived biomaterialsosteogenic differentiation |
| spellingShingle | Eleonora Stanca Nadia Calabriso Laura Giannotti Paola Nitti Fabrizio Damiano Benedetta Di Chiara Stanca Maria Annunziata Carluccio Giuseppe Egidio De Benedetto Christian Demitri Andrea Palermo Franco Ferrante Luisa Siculella Alessio Rochira Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential International Journal of Molecular Sciences CGF growth factor stem cells blood-derived biomaterials osteogenic differentiation |
| title | Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential |
| title_full | Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential |
| title_fullStr | Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential |
| title_full_unstemmed | Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential |
| title_short | Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential |
| title_sort | analysis of cgf biomolecules structure and cell population characterization of the stemness features of cgf cells and osteogenic potential |
| topic | CGF growth factor stem cells blood-derived biomaterials osteogenic differentiation |
| url | https://www.mdpi.com/1422-0067/22/16/8867 |
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