An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo
Abstract Backgroud Drinking water contamination with hexavalent chromium [Cr (VI)] has become one of the most serious public health problems, thus the investigation of Cr (VI)-induced hepatotoxicity has attracted much attention in recent years. Methods In the present study, by determining the indice...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Journal of Occupational Medicine and Toxicology |
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| Online Access: | http://link.springer.com/article/10.1186/s12995-017-0161-x |
| _version_ | 1811262387131514880 |
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| author | Xiali Zhong Ming Zeng Huanfeng Bian Caigao Zhong Fang Xiao |
| author_facet | Xiali Zhong Ming Zeng Huanfeng Bian Caigao Zhong Fang Xiao |
| author_sort | Xiali Zhong |
| collection | DOAJ |
| description | Abstract Backgroud Drinking water contamination with hexavalent chromium [Cr (VI)] has become one of the most serious public health problems, thus the investigation of Cr (VI)-induced hepatotoxicity has attracted much attention in recent years. Methods In the present study, by determining the indices of hepatotoxicity induced by Cr (VI), the source of accumulated reactive oxygen species (ROS), and the protective effect of the antioxidant Vitamin C (Vit C), we explored the mechanisms involved in Cr (VI)-induced hepatotoxicity in vitro and in vivo. Results We found Cr (VI) caused hepatotoxicity characterized by the alterations of several enzymatic and cytokine markers including aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukine-1β (IL-1β), and tumor necrosis factor-α (TNF-α), etc. ROS production after Cr (VI) exposure was origins from the inhibition of electron transfer chain (ETC) and antioxidant system. Vit C inhibited ROS accumulation thus protected against Cr (VI)-induced hepatotoxicity in L-02 hepatocytes and in the rat model. Conclusions We concluded that ROS played a role in Cr (VI)-induced hepatotoxicity and Vit C exhibited protective effect. Our current data provides important clues for studying the mechanisms involved in Cr (VI)-induced liver injury, and may be of great help to develop therapeutic strategies for prevention and treatment of liver diseases involving ROS accumulation for occupational exposure population. |
| first_indexed | 2024-04-12T19:24:29Z |
| format | Article |
| id | doaj.art-e5efb581cd894e8aa342e56d4a2f6041 |
| institution | Directory Open Access Journal |
| issn | 1745-6673 |
| language | English |
| last_indexed | 2024-04-12T19:24:29Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Occupational Medicine and Toxicology |
| spelling | doaj.art-e5efb581cd894e8aa342e56d4a2f60412022-12-22T03:19:31ZengBMCJournal of Occupational Medicine and Toxicology1745-66732017-06-0112111210.1186/s12995-017-0161-xAn evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivoXiali Zhong0Ming Zeng1Huanfeng Bian2Caigao Zhong3Fang Xiao4Department of Health Toxicology, Xiangya School of Public Health, Central South UniversityDepartment of Health Toxicology, Xiangya School of Public Health, Central South UniversityShajing Institution of Health Supervision of Baoan DistrictDepartment of Health Toxicology, Xiangya School of Public Health, Central South UniversityDepartment of Health Toxicology, Xiangya School of Public Health, Central South UniversityAbstract Backgroud Drinking water contamination with hexavalent chromium [Cr (VI)] has become one of the most serious public health problems, thus the investigation of Cr (VI)-induced hepatotoxicity has attracted much attention in recent years. Methods In the present study, by determining the indices of hepatotoxicity induced by Cr (VI), the source of accumulated reactive oxygen species (ROS), and the protective effect of the antioxidant Vitamin C (Vit C), we explored the mechanisms involved in Cr (VI)-induced hepatotoxicity in vitro and in vivo. Results We found Cr (VI) caused hepatotoxicity characterized by the alterations of several enzymatic and cytokine markers including aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukine-1β (IL-1β), and tumor necrosis factor-α (TNF-α), etc. ROS production after Cr (VI) exposure was origins from the inhibition of electron transfer chain (ETC) and antioxidant system. Vit C inhibited ROS accumulation thus protected against Cr (VI)-induced hepatotoxicity in L-02 hepatocytes and in the rat model. Conclusions We concluded that ROS played a role in Cr (VI)-induced hepatotoxicity and Vit C exhibited protective effect. Our current data provides important clues for studying the mechanisms involved in Cr (VI)-induced liver injury, and may be of great help to develop therapeutic strategies for prevention and treatment of liver diseases involving ROS accumulation for occupational exposure population.http://link.springer.com/article/10.1186/s12995-017-0161-xHexavalent chromium [Cr (VI)]Reactive oxygen species (ROS)HepatotoxicityVitamin C (Vit C)Mitochondrial respiratory chain complex I (MRCC I) |
| spellingShingle | Xiali Zhong Ming Zeng Huanfeng Bian Caigao Zhong Fang Xiao An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo Journal of Occupational Medicine and Toxicology Hexavalent chromium [Cr (VI)] Reactive oxygen species (ROS) Hepatotoxicity Vitamin C (Vit C) Mitochondrial respiratory chain complex I (MRCC I) |
| title | An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| title_full | An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| title_fullStr | An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| title_full_unstemmed | An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| title_short | An evaluation of the protective role of vitamin C in reactive oxygen species-induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| title_sort | evaluation of the protective role of vitamin c in reactive oxygen species induced hepatotoxicity due to hexavalent chromium in vitro and in vivo |
| topic | Hexavalent chromium [Cr (VI)] Reactive oxygen species (ROS) Hepatotoxicity Vitamin C (Vit C) Mitochondrial respiratory chain complex I (MRCC I) |
| url | http://link.springer.com/article/10.1186/s12995-017-0161-x |
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