Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles
Amatoxin poisoning induces delayed-onset acute liver failure, which are responsible for more than 90% of deaths in mushroom poisoning. It has been postulated from animal and human studies that biliary drainage interrupting enterohepatic amatoxin circulation may affect amatoxin poisoning. Dogs were r...
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MDPI AG
2018-05-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/6/215 |
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| author | Jian Sun Yu-Tao Zhang Yu-Min Niu Hai-Jiao Li Yu Yin Yi-Zhe Zhang Pei-Bin Ma Jing Zhou Jun-Jia Lu Hong-Shun Zhang Cheng-Ye Sun |
| author_facet | Jian Sun Yu-Tao Zhang Yu-Min Niu Hai-Jiao Li Yu Yin Yi-Zhe Zhang Pei-Bin Ma Jing Zhou Jun-Jia Lu Hong-Shun Zhang Cheng-Ye Sun |
| author_sort | Jian Sun |
| collection | DOAJ |
| description | Amatoxin poisoning induces delayed-onset acute liver failure, which are responsible for more than 90% of deaths in mushroom poisoning. It has been postulated from animal and human studies that biliary drainage interrupting enterohepatic amatoxin circulation may affect amatoxin poisoning. Dogs were randomly divided into four groups of six animals each. In 20 mg/kg and 60 mg/kg with biliary drainage groups, after accepting bile drainage operation, beagles were fed Amanita exitialis powder (20 or 60 mg/kg) in starch capsules. In control and bile drainage groups, the beagle dogs were fed with empty capsules. They were assessed for toxicity signs, biochemical and pathological changes, and peptide toxins in plasma, urine and bile. The data were directly compared with those from our published studies on Amanita exitialis-exposed beagles without biliary drainage. Amatoxins were rapidly absorbed and eliminated from plasma after Amanita exitialis ingestion. Amatoxins in 0–1-day urine accounted for more than 90% of the total urine excretion, and amatoxins in bile accounted for less than 20% of the total urine and bile excretion. The dogs with biliary drainage showed less severe toxicity signs and biochemical and pathological changes and much lower internal exposure than dogs without biliary drainage. Biliary drainage caused a more than 70% reduction in intestinal amatoxin absorption and could reduce amatoxin absorption from the gastrointestinal tract. |
| first_indexed | 2024-12-10T07:47:06Z |
| format | Article |
| id | doaj.art-e5f0352e12d94fcfbf36ae6afddad772 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-12-10T07:47:06Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-e5f0352e12d94fcfbf36ae6afddad7722022-12-22T01:57:10ZengMDPI AGToxins2072-66512018-05-0110621510.3390/toxins10060215toxins10060215Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in BeaglesJian Sun0Yu-Tao Zhang1Yu-Min Niu2Hai-Jiao Li3Yu Yin4Yi-Zhe Zhang5Pei-Bin Ma6Jing Zhou7Jun-Jia Lu8Hong-Shun Zhang9Cheng-Ye Sun10National Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaNational Institute of Occupational Health and Poison Control, Chinese Centre for Disease Control and Prevention, Beijing 100050, ChinaAmatoxin poisoning induces delayed-onset acute liver failure, which are responsible for more than 90% of deaths in mushroom poisoning. It has been postulated from animal and human studies that biliary drainage interrupting enterohepatic amatoxin circulation may affect amatoxin poisoning. Dogs were randomly divided into four groups of six animals each. In 20 mg/kg and 60 mg/kg with biliary drainage groups, after accepting bile drainage operation, beagles were fed Amanita exitialis powder (20 or 60 mg/kg) in starch capsules. In control and bile drainage groups, the beagle dogs were fed with empty capsules. They were assessed for toxicity signs, biochemical and pathological changes, and peptide toxins in plasma, urine and bile. The data were directly compared with those from our published studies on Amanita exitialis-exposed beagles without biliary drainage. Amatoxins were rapidly absorbed and eliminated from plasma after Amanita exitialis ingestion. Amatoxins in 0–1-day urine accounted for more than 90% of the total urine excretion, and amatoxins in bile accounted for less than 20% of the total urine and bile excretion. The dogs with biliary drainage showed less severe toxicity signs and biochemical and pathological changes and much lower internal exposure than dogs without biliary drainage. Biliary drainage caused a more than 70% reduction in intestinal amatoxin absorption and could reduce amatoxin absorption from the gastrointestinal tract.http://www.mdpi.com/2072-6651/10/6/215Amanita exitialisamatoxinsbeaglesbile drainageurine |
| spellingShingle | Jian Sun Yu-Tao Zhang Yu-Min Niu Hai-Jiao Li Yu Yin Yi-Zhe Zhang Pei-Bin Ma Jing Zhou Jun-Jia Lu Hong-Shun Zhang Cheng-Ye Sun Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles Toxins Amanita exitialis amatoxins beagles bile drainage urine |
| title | Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles |
| title_full | Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles |
| title_fullStr | Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles |
| title_full_unstemmed | Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles |
| title_short | Effect of Biliary Drainage on the Toxicity and Toxicokinetics of Amanita exitialis in Beagles |
| title_sort | effect of biliary drainage on the toxicity and toxicokinetics of amanita exitialis in beagles |
| topic | Amanita exitialis amatoxins beagles bile drainage urine |
| url | http://www.mdpi.com/2072-6651/10/6/215 |
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