Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities
ABSTRACT Poplar anthracnose caused by Colletotrichum gloeosporioides is a common disease affecting poplars globally that causes the destruction and alteration of poplar phyllosphere microbial communities; however, few studies have investigated these communities. Therefore, in this study, three speci...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04603-22 |
| _version_ | 1827925121688403968 |
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| author | Linxuan Zhang Fanli Meng Wei Ge Yue Ren Hangbin Bao Chengming Tian |
| author_facet | Linxuan Zhang Fanli Meng Wei Ge Yue Ren Hangbin Bao Chengming Tian |
| author_sort | Linxuan Zhang |
| collection | DOAJ |
| description | ABSTRACT Poplar anthracnose caused by Colletotrichum gloeosporioides is a common disease affecting poplars globally that causes the destruction and alteration of poplar phyllosphere microbial communities; however, few studies have investigated these communities. Therefore, in this study, three species of poplar with different resistances were investigated to explore the effects of Colletotrichum gloeosporioides and poplar secondary metabolites on the composition of poplar phyllosphere microbial communities. Evaluation of the phyllosphere microbial communities before and after inoculation of the poplars with C. gloeosporioides revealed that both bacterial and fungal OTUs decreased after inoculation. Among bacteria, the most abundant genera were Bacillus, Plesiomonas, Pseudomonas, Rhizobium, Cetobacterium, Streptococcus, Massilia, and Shigella for all poplar species. Among fungi, the most abundant genera before inoculation were Cladosporium, Aspergillus, Fusarium, Mortierella, and Colletotrichum, while Colletotrichum was the main genus after inoculation. The inoculation of pathogens may regulate the phyllosphere microorganisms by affecting the secondary metabolites of plants. We investigated metabolite contents in the phyllosphere before and after the inoculation of the three poplar species, as well as the effects of flavonoids, organic acids, coumarins, and indoles on poplar phyllosphere microbial communities. We speculated that coumarin had the greatest recruitment effect on phyllosphere microorganisms, followed by organic acids through regression analysis. Overall, our results provide a foundation for subsequent screening of antagonistic bacteria and fungi against poplar anthracnose and investigations of the mechanism by which poplar phyllosphere microorganisms are recruited. IMPORTANCE Our findings revealed that the inoculation of Colletotrichum gloeosporioides has a greater effect on the fungal community than the bacterial community. In addition, coumarins, organic acids, and flavonoids may have recruitment effects on phyllosphere microorganisms, while indoles may have inhibitory effects on these organisms. These findings may provide the theoretical basis for the prevention and control of poplar anthracnose. |
| first_indexed | 2024-03-13T05:20:40Z |
| format | Article |
| id | doaj.art-e5f120c6c1dc4375b4dbdfb38401ed32 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:20:40Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-e5f120c6c1dc4375b4dbdfb38401ed322023-06-15T13:18:32ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.04603-22Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial CommunitiesLinxuan Zhang0Fanli Meng1Wei Ge2Yue Ren3Hangbin Bao4Chengming Tian5The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaThe Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaThe Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaThe Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaThe Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaThe Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, ChinaABSTRACT Poplar anthracnose caused by Colletotrichum gloeosporioides is a common disease affecting poplars globally that causes the destruction and alteration of poplar phyllosphere microbial communities; however, few studies have investigated these communities. Therefore, in this study, three species of poplar with different resistances were investigated to explore the effects of Colletotrichum gloeosporioides and poplar secondary metabolites on the composition of poplar phyllosphere microbial communities. Evaluation of the phyllosphere microbial communities before and after inoculation of the poplars with C. gloeosporioides revealed that both bacterial and fungal OTUs decreased after inoculation. Among bacteria, the most abundant genera were Bacillus, Plesiomonas, Pseudomonas, Rhizobium, Cetobacterium, Streptococcus, Massilia, and Shigella for all poplar species. Among fungi, the most abundant genera before inoculation were Cladosporium, Aspergillus, Fusarium, Mortierella, and Colletotrichum, while Colletotrichum was the main genus after inoculation. The inoculation of pathogens may regulate the phyllosphere microorganisms by affecting the secondary metabolites of plants. We investigated metabolite contents in the phyllosphere before and after the inoculation of the three poplar species, as well as the effects of flavonoids, organic acids, coumarins, and indoles on poplar phyllosphere microbial communities. We speculated that coumarin had the greatest recruitment effect on phyllosphere microorganisms, followed by organic acids through regression analysis. Overall, our results provide a foundation for subsequent screening of antagonistic bacteria and fungi against poplar anthracnose and investigations of the mechanism by which poplar phyllosphere microorganisms are recruited. IMPORTANCE Our findings revealed that the inoculation of Colletotrichum gloeosporioides has a greater effect on the fungal community than the bacterial community. In addition, coumarins, organic acids, and flavonoids may have recruitment effects on phyllosphere microorganisms, while indoles may have inhibitory effects on these organisms. These findings may provide the theoretical basis for the prevention and control of poplar anthracnose.https://journals.asm.org/doi/10.1128/spectrum.04603-22poplar anthracnoseColletotrichum gloeosporioidesphyllosphere microbial communitysecondary metabolites |
| spellingShingle | Linxuan Zhang Fanli Meng Wei Ge Yue Ren Hangbin Bao Chengming Tian Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities Microbiology Spectrum poplar anthracnose Colletotrichum gloeosporioides phyllosphere microbial community secondary metabolites |
| title | Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities |
| title_full | Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities |
| title_fullStr | Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities |
| title_full_unstemmed | Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities |
| title_short | Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities |
| title_sort | effects of colletotrichum gloeosporioides and poplar secondary metabolites on the composition of poplar phyllosphere microbial communities |
| topic | poplar anthracnose Colletotrichum gloeosporioides phyllosphere microbial community secondary metabolites |
| url | https://journals.asm.org/doi/10.1128/spectrum.04603-22 |
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