Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition
In this study, the effects of a homogenous porphyran from <i>Porphyra haitanensis</i> (PHP) on the intestinal barrier and gut microbiota were investigated. The results showed that oral administration of PHP resulted in a higher luminal moisture content and a lower pH environment for the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/5/265 |
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| author | Sathuvan Malairaj Suresh Veeraperumal Wanzi Yao Mugesh Subramanian Karsoon Tan Saiyi Zhong Kit-Leong Cheong |
| author_facet | Sathuvan Malairaj Suresh Veeraperumal Wanzi Yao Mugesh Subramanian Karsoon Tan Saiyi Zhong Kit-Leong Cheong |
| author_sort | Sathuvan Malairaj |
| collection | DOAJ |
| description | In this study, the effects of a homogenous porphyran from <i>Porphyra haitanensis</i> (PHP) on the intestinal barrier and gut microbiota were investigated. The results showed that oral administration of PHP resulted in a higher luminal moisture content and a lower pH environment for the growth of beneficial bacteria in the colon of mice. PHP significantly increased the production of total short-chain fatty acids during the fermentation process. PHP made the intestinal epithelial cells of mice arrange more tidily and tightly with a significant increase in mucosal thickness. PHP also increased the amount of mucin-producing goblet cells and the expression of mucin in the colon, which maintained the structure and function of the intestinal mucosal barrier. Moreover, PHP up-regulated the expression of tight junctions including ZO-1 and occludin, improving the intestinal physical barrier function. The results of 16S rRNA sequencing showed that PHP regulated the composition of gut microbiota in mice, increasing the richness and diversity of gut microbiota and the ratio of Firmicutes to Bacteroidetes. This study revealed that the intake of PHP is beneficial for the gastrointestinal tract and PHP could be a potential source of prebiotics in the functional food and pharmaceutical industries. |
| first_indexed | 2024-03-11T03:33:57Z |
| format | Article |
| id | doaj.art-e5f3114c172f43ec96a4e1de7e688cd9 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T03:33:57Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e5f3114c172f43ec96a4e1de7e688cd92023-11-18T02:12:34ZengMDPI AGMarine Drugs1660-33972023-04-0121526510.3390/md21050265Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota CompositionSathuvan Malairaj0Suresh Veeraperumal1Wanzi Yao2Mugesh Subramanian3Karsoon Tan4Saiyi Zhong5Kit-Leong Cheong6Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Biology, College of Science, Shantou University, Shantou 515063, ChinaResearch and Development Center, Genexia Bioserv, Chennai 600045, Tamilnadu, IndiaGuangxi Key Laboratory of Beibu Gulf Biodiversity Conservation, Beibu Gulf University, Qinzhou 535011, ChinaGuangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaIn this study, the effects of a homogenous porphyran from <i>Porphyra haitanensis</i> (PHP) on the intestinal barrier and gut microbiota were investigated. The results showed that oral administration of PHP resulted in a higher luminal moisture content and a lower pH environment for the growth of beneficial bacteria in the colon of mice. PHP significantly increased the production of total short-chain fatty acids during the fermentation process. PHP made the intestinal epithelial cells of mice arrange more tidily and tightly with a significant increase in mucosal thickness. PHP also increased the amount of mucin-producing goblet cells and the expression of mucin in the colon, which maintained the structure and function of the intestinal mucosal barrier. Moreover, PHP up-regulated the expression of tight junctions including ZO-1 and occludin, improving the intestinal physical barrier function. The results of 16S rRNA sequencing showed that PHP regulated the composition of gut microbiota in mice, increasing the richness and diversity of gut microbiota and the ratio of Firmicutes to Bacteroidetes. This study revealed that the intake of PHP is beneficial for the gastrointestinal tract and PHP could be a potential source of prebiotics in the functional food and pharmaceutical industries.https://www.mdpi.com/1660-3397/21/5/265<i>Porphyra haitanensis</i>porphyranintestinal barriergut microbiota |
| spellingShingle | Sathuvan Malairaj Suresh Veeraperumal Wanzi Yao Mugesh Subramanian Karsoon Tan Saiyi Zhong Kit-Leong Cheong Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition Marine Drugs <i>Porphyra haitanensis</i> porphyran intestinal barrier gut microbiota |
| title | Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition |
| title_full | Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition |
| title_fullStr | Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition |
| title_full_unstemmed | Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition |
| title_short | Porphyran from <em>Porphyra haitanensis</em> Enhances Intestinal Barrier Function and Regulates Gut Microbiota Composition |
| title_sort | porphyran from em porphyra haitanensis em enhances intestinal barrier function and regulates gut microbiota composition |
| topic | <i>Porphyra haitanensis</i> porphyran intestinal barrier gut microbiota |
| url | https://www.mdpi.com/1660-3397/21/5/265 |
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