Gut microbiota transplantation for colonization of germ-free mice
Summary: The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721003178 |
| _version_ | 1818664315858714624 |
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| author | Jocelyn M. Choo Geraint B. Rogers |
| author_facet | Jocelyn M. Choo Geraint B. Rogers |
| author_sort | Jocelyn M. Choo |
| collection | DOAJ |
| description | Summary: The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery.For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021). |
| first_indexed | 2024-12-17T05:30:48Z |
| format | Article |
| id | doaj.art-e5f5dfbc3ea84b798bbcc9ce9e126c3f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T05:30:48Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-e5f5dfbc3ea84b798bbcc9ce9e126c3f2022-12-21T22:01:44ZengElsevierSTAR Protocols2666-16672021-09-0123100610Gut microbiota transplantation for colonization of germ-free miceJocelyn M. Choo0Geraint B. Rogers1Microbiome and Host Health, South Australia Health and Medical Research Institute, Adelaide 5000, Australia; Microbiome Research Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park 5042, Australia; Corresponding authorMicrobiome and Host Health, South Australia Health and Medical Research Institute, Adelaide 5000, Australia; Microbiome Research Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park 5042, Australia; Corresponding authorSummary: The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery.For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).http://www.sciencedirect.com/science/article/pii/S2666166721003178Sequence analysisMicrobiologyModel OrganismsMolecular Biology |
| spellingShingle | Jocelyn M. Choo Geraint B. Rogers Gut microbiota transplantation for colonization of germ-free mice STAR Protocols Sequence analysis Microbiology Model Organisms Molecular Biology |
| title | Gut microbiota transplantation for colonization of germ-free mice |
| title_full | Gut microbiota transplantation for colonization of germ-free mice |
| title_fullStr | Gut microbiota transplantation for colonization of germ-free mice |
| title_full_unstemmed | Gut microbiota transplantation for colonization of germ-free mice |
| title_short | Gut microbiota transplantation for colonization of germ-free mice |
| title_sort | gut microbiota transplantation for colonization of germ free mice |
| topic | Sequence analysis Microbiology Model Organisms Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721003178 |
| work_keys_str_mv | AT jocelynmchoo gutmicrobiotatransplantationforcolonizationofgermfreemice AT geraintbrogers gutmicrobiotatransplantationforcolonizationofgermfreemice |