Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro
Background and aims: VL (Viseral Leishmaniosis) the second- most dreaded parasitic disease after malaria is currently endemic in 88 countries. Artemisia sieberi is native medicinal plants in Iran and their effects are scientifically proven to be effective on leishmaniasis. The aim of this study was...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2017-04-01
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| Series: | Advanced Herbal Medicine |
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| Online Access: | http://herbmed.skums.ac.ir/article_35215_9e8f06baa20fbad7c03071d924bfd9b5.pdf |
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| author | elham moghaddas Mohaddeseh Abouhosseini Tabari Mohammad Reza Youssefi Mohammad Amin Ebrahimi Nilofar Nabavi Mousavi ali naseri |
| author_facet | elham moghaddas Mohaddeseh Abouhosseini Tabari Mohammad Reza Youssefi Mohammad Amin Ebrahimi Nilofar Nabavi Mousavi ali naseri |
| author_sort | elham moghaddas |
| collection | DOAJ |
| description | Background and aims: VL (Viseral Leishmaniosis) the second- most dreaded parasitic disease after malaria is currently endemic in 88 countries. Artemisia sieberi is native medicinal plants in Iran and their effects are scientifically proven to be effective on leishmaniasis. The aim of this study was to investigate antileishmanial effects of A.sieberi essential oil on Leishmania infantum in vitro. This is the first application of A. sieberi for treatment of L.infantum. Methods: Promastigotes of L. infantum were treated by A. sieberi in 1, 5, 10, 15, 20 μg/ml concentration. MTT test ([3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] was done in 48h after treatment to determine the effect on promastigote viability. The data were analyzed by SPSS, and using one-way ANOVA and Mann Whitney tests. Results: Fewer than 15 μg /ml concentrations of A. sieberi essential oil were no appreciable effect on the parasite. A dose of 15, 20 μg/ml showed growth inhibitory on L. infantum in 24h/48h compared to control group (P<0.05). Conclusion: The A. sieberi essential oil had antileishmanial effects against L.infantum in vitro. Therefore, they might be a reliable source for preparation of new drugs. More in vivo investigations are required to clarify details of effects of A.sieberi on leishmania spp and analysis of its natural components. |
| first_indexed | 2024-12-13T12:58:40Z |
| format | Article |
| id | doaj.art-e5ff151685344650b49b02918a40117a |
| institution | Directory Open Access Journal |
| issn | 2423-3579 2423-3579 |
| language | English |
| last_indexed | 2024-12-13T12:58:40Z |
| publishDate | 2017-04-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Advanced Herbal Medicine |
| spelling | doaj.art-e5ff151685344650b49b02918a40117a2022-12-21T23:45:06ZengShahrekord University of Medical SciencesAdvanced Herbal Medicine2423-35792423-35792017-04-0132404635215Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitroelham moghaddas0Mohaddeseh Abouhosseini Tabari1Mohammad Reza Youssefi2Mohammad Amin Ebrahimi3Nilofar Nabavi Mousavi4ali naseri5Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Faculty of Veterinary Medicine, Amol University of Special ModernTechnologies, Amol, IranDepartment of Veterinary Parasitology, Islamic Azad University, Babol-Branch, Babol, IranYoung Researcher and Elite Club, Babol Branch, Islamic Azad University, Babol, IranYoung Researcher and Elite Club, Babol Branch, Islamic Azad University, Babol, IranDepartment of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Background and aims: VL (Viseral Leishmaniosis) the second- most dreaded parasitic disease after malaria is currently endemic in 88 countries. Artemisia sieberi is native medicinal plants in Iran and their effects are scientifically proven to be effective on leishmaniasis. The aim of this study was to investigate antileishmanial effects of A.sieberi essential oil on Leishmania infantum in vitro. This is the first application of A. sieberi for treatment of L.infantum. Methods: Promastigotes of L. infantum were treated by A. sieberi in 1, 5, 10, 15, 20 μg/ml concentration. MTT test ([3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] was done in 48h after treatment to determine the effect on promastigote viability. The data were analyzed by SPSS, and using one-way ANOVA and Mann Whitney tests. Results: Fewer than 15 μg /ml concentrations of A. sieberi essential oil were no appreciable effect on the parasite. A dose of 15, 20 μg/ml showed growth inhibitory on L. infantum in 24h/48h compared to control group (P<0.05). Conclusion: The A. sieberi essential oil had antileishmanial effects against L.infantum in vitro. Therefore, they might be a reliable source for preparation of new drugs. More in vivo investigations are required to clarify details of effects of A.sieberi on leishmania spp and analysis of its natural components.http://herbmed.skums.ac.ir/article_35215_9e8f06baa20fbad7c03071d924bfd9b5.pdfleishmania infantumartemisiapromastigotein vitro technique |
| spellingShingle | elham moghaddas Mohaddeseh Abouhosseini Tabari Mohammad Reza Youssefi Mohammad Amin Ebrahimi Nilofar Nabavi Mousavi ali naseri Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro Advanced Herbal Medicine leishmania infantum artemisia promastigote in vitro technique |
| title | Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro |
| title_full | Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro |
| title_fullStr | Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro |
| title_full_unstemmed | Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro |
| title_short | Antileashmanial activity of Artemisia sieberi essential oil against Leishmania infantum in vitro |
| title_sort | antileashmanial activity of artemisia sieberi essential oil against leishmania infantum in vitro |
| topic | leishmania infantum artemisia promastigote in vitro technique |
| url | http://herbmed.skums.ac.ir/article_35215_9e8f06baa20fbad7c03071d924bfd9b5.pdf |
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