Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics
Abstract Listeria monocytogenes is an important foodborne pathogen, which is able to persist in the food production environments. The presence of these bacteria in different niches makes them a potential threat for public health. In the present review, the current information on the classical and al...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.2910 |
| _version_ | 1798036074288644096 |
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| author | Jacek Osek Beata Lachtara Kinga Wieczorek |
| author_facet | Jacek Osek Beata Lachtara Kinga Wieczorek |
| author_sort | Jacek Osek |
| collection | DOAJ |
| description | Abstract Listeria monocytogenes is an important foodborne pathogen, which is able to persist in the food production environments. The presence of these bacteria in different niches makes them a potential threat for public health. In the present review, the current information on the classical and alternative methods used for isolation and identification of L. monocytogenes in food have been described. Although these techniques are usually simple, standardized, inexpensive, and are routinely used in many food testing laboratories, several alternative molecular‐based approaches for the bacteria detection in food and food production environments have been developed. They are characterized by the high sample throughput, a short time of analysis, and cost‐effectiveness. However, these methods are important for the routine testing toward the presence and number of L. monocytogenes, but are not suitable for characteristics and typing of the bacterial isolates, which are crucial in the study of listeriosis infections. For these purposes, novel approaches, with a high discriminatory power to genetically distinguish the strains during epidemiological studies, have been developed, e.g., whole‐genome sequence‐based techniques such as NGS which provide an opportunity to perform comparison between strains of the same species. In the present review, we have shown a short description of the principles of microbiological, alternative, and modern methods of detection of L. monocytogenes in foods and characterization of the isolates for epidemiological purposes. According to our knowledge, similar comprehensive papers on such subject have not been recently published, and we hope that the current review may be interesting for research communities. |
| first_indexed | 2024-04-11T21:07:19Z |
| format | Article |
| id | doaj.art-e602b22f90c74189859c547fdea7d2d5 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-11T21:07:19Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-e602b22f90c74189859c547fdea7d2d52022-12-22T04:03:14ZengWileyFood Science & Nutrition2048-71772022-09-011092825285410.1002/fsn3.2910Listeria monocytogenes in foods—From culture identification to whole‐genome characteristicsJacek Osek0Beata Lachtara1Kinga Wieczorek2Department of Hygiene of Food of Animal Origin National Veterinary Research Institute Puławy PolandDepartment of Hygiene of Food of Animal Origin National Veterinary Research Institute Puławy PolandDepartment of Hygiene of Food of Animal Origin National Veterinary Research Institute Puławy PolandAbstract Listeria monocytogenes is an important foodborne pathogen, which is able to persist in the food production environments. The presence of these bacteria in different niches makes them a potential threat for public health. In the present review, the current information on the classical and alternative methods used for isolation and identification of L. monocytogenes in food have been described. Although these techniques are usually simple, standardized, inexpensive, and are routinely used in many food testing laboratories, several alternative molecular‐based approaches for the bacteria detection in food and food production environments have been developed. They are characterized by the high sample throughput, a short time of analysis, and cost‐effectiveness. However, these methods are important for the routine testing toward the presence and number of L. monocytogenes, but are not suitable for characteristics and typing of the bacterial isolates, which are crucial in the study of listeriosis infections. For these purposes, novel approaches, with a high discriminatory power to genetically distinguish the strains during epidemiological studies, have been developed, e.g., whole‐genome sequence‐based techniques such as NGS which provide an opportunity to perform comparison between strains of the same species. In the present review, we have shown a short description of the principles of microbiological, alternative, and modern methods of detection of L. monocytogenes in foods and characterization of the isolates for epidemiological purposes. According to our knowledge, similar comprehensive papers on such subject have not been recently published, and we hope that the current review may be interesting for research communities.https://doi.org/10.1002/fsn3.2910classical methodsgenotypingidentificationListeria monocytogenesmolecular methods |
| spellingShingle | Jacek Osek Beata Lachtara Kinga Wieczorek Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics Food Science & Nutrition classical methods genotyping identification Listeria monocytogenes molecular methods |
| title | Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics |
| title_full | Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics |
| title_fullStr | Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics |
| title_full_unstemmed | Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics |
| title_short | Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics |
| title_sort | listeria monocytogenes in foods from culture identification to whole genome characteristics |
| topic | classical methods genotyping identification Listeria monocytogenes molecular methods |
| url | https://doi.org/10.1002/fsn3.2910 |
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