Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition
Abstract Background Schistosomiasis is a prevalent but neglected tropical disease caused by parasitic trematodes of the genus Schistosoma, with the primary disease-causing species being S. haematobium, S. mansoni and S. japonicum. Male–female pairing of schistosomes is necessary for sexual maturity...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3672-8 |
| _version_ | 1828403807014354944 |
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| author | Fengchun Liu Han Ding Jiaming Tian Congyu Zhou Fei Yang Wei Shao Yinan Du Xin Hou Cuiping Ren Jijia Shen Miao Liu |
| author_facet | Fengchun Liu Han Ding Jiaming Tian Congyu Zhou Fei Yang Wei Shao Yinan Du Xin Hou Cuiping Ren Jijia Shen Miao Liu |
| author_sort | Fengchun Liu |
| collection | DOAJ |
| description | Abstract Background Schistosomiasis is a prevalent but neglected tropical disease caused by parasitic trematodes of the genus Schistosoma, with the primary disease-causing species being S. haematobium, S. mansoni and S. japonicum. Male–female pairing of schistosomes is necessary for sexual maturity and the production of a large number of eggs, which are primarily responsible for schistosomiasis dissemination and pathology. Methods Here, we used microarray hybridization, bioinformatics, quantitative PCR, in situ hybridization and gene silencing assays to identify genes that play critical roles in S. japonicum reproduction biology, particularly in vitellarium development, a process that affects male–female pairing, sexual maturation and subsequent egg production. Results Microarray hybridization analyses generated a comprehensive set of genes differentially transcribed before and after male–female pairing. Although the transcript profiles of females were similar 16 and 18 days after host infection, marked gene expression changes were observed at 24 days. The 30 most abundantly transcribed genes on day 24 included those associated with vitellarium development. Among these, the gene for female-specific 800 (fs800) was substantially upregulated. Our in situ hybridization results in female S. japonicum indicated that Sjfs800 mRNA was observed only in the vitellarium, localized in mature vitelline cells. Knocking down the Sjfs800 gene in female S. japonicum by approximately 60% reduced the number of mature vitelline cells, decreased rates of pairing and oviposition, and decreased the number of eggs produced in each male–female pairing by about 50%. Conclusions These results indicate that Sjfs800 may play a role in vitellarium development and egg production in S. japonicum and suggest that Sjfs800 regulation may provide a novel approach for the prevention or treatment of schistosomiasis. |
| first_indexed | 2024-12-10T10:23:54Z |
| format | Article |
| id | doaj.art-e602b6f1f16d48f6b245093fb6c6f9ec |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-10T10:23:54Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-e602b6f1f16d48f6b245093fb6c6f9ec2022-12-22T01:52:46ZengBMCParasites & Vectors1756-33052019-08-0112111210.1186/s13071-019-3672-8Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and ovipositionFengchun Liu0Han Ding1Jiaming Tian2Congyu Zhou3Fei Yang4Wei Shao5Yinan Du6Xin Hou7Cuiping Ren8Jijia Shen9Miao Liu10Department of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical UniversityAbstract Background Schistosomiasis is a prevalent but neglected tropical disease caused by parasitic trematodes of the genus Schistosoma, with the primary disease-causing species being S. haematobium, S. mansoni and S. japonicum. Male–female pairing of schistosomes is necessary for sexual maturity and the production of a large number of eggs, which are primarily responsible for schistosomiasis dissemination and pathology. Methods Here, we used microarray hybridization, bioinformatics, quantitative PCR, in situ hybridization and gene silencing assays to identify genes that play critical roles in S. japonicum reproduction biology, particularly in vitellarium development, a process that affects male–female pairing, sexual maturation and subsequent egg production. Results Microarray hybridization analyses generated a comprehensive set of genes differentially transcribed before and after male–female pairing. Although the transcript profiles of females were similar 16 and 18 days after host infection, marked gene expression changes were observed at 24 days. The 30 most abundantly transcribed genes on day 24 included those associated with vitellarium development. Among these, the gene for female-specific 800 (fs800) was substantially upregulated. Our in situ hybridization results in female S. japonicum indicated that Sjfs800 mRNA was observed only in the vitellarium, localized in mature vitelline cells. Knocking down the Sjfs800 gene in female S. japonicum by approximately 60% reduced the number of mature vitelline cells, decreased rates of pairing and oviposition, and decreased the number of eggs produced in each male–female pairing by about 50%. Conclusions These results indicate that Sjfs800 may play a role in vitellarium development and egg production in S. japonicum and suggest that Sjfs800 regulation may provide a novel approach for the prevention or treatment of schistosomiasis.http://link.springer.com/article/10.1186/s13071-019-3672-8Schistosoma japonicumSjfs800Vitellarium developmentEgg production |
| spellingShingle | Fengchun Liu Han Ding Jiaming Tian Congyu Zhou Fei Yang Wei Shao Yinan Du Xin Hou Cuiping Ren Jijia Shen Miao Liu Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition Parasites & Vectors Schistosoma japonicum Sjfs800 Vitellarium development Egg production |
| title | Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition |
| title_full | Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition |
| title_fullStr | Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition |
| title_full_unstemmed | Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition |
| title_short | Differential gene expression, including Sjfs800, in Schistosoma japonicum females at pre-pairing, initial pairing and oviposition |
| title_sort | differential gene expression including sjfs800 in schistosoma japonicum females at pre pairing initial pairing and oviposition |
| topic | Schistosoma japonicum Sjfs800 Vitellarium development Egg production |
| url | http://link.springer.com/article/10.1186/s13071-019-3672-8 |
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