Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China
Background: Spontaneous abortion is the most common complication of early pregnancy. In this study, we aim to investigate the clinical application value of genetic diagnosis using single nucleotide polymorphism (SNP) microarray analysis on the products of conception and to characterize the types of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1319624/full |
| _version_ | 1827583659777982464 |
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| author | ShuYuan Xue ShuYuan Xue LiXia Wang Jie Wei YuTong Liu GuiFeng Ding PengGao Dai |
| author_facet | ShuYuan Xue ShuYuan Xue LiXia Wang Jie Wei YuTong Liu GuiFeng Ding PengGao Dai |
| author_sort | ShuYuan Xue |
| collection | DOAJ |
| description | Background: Spontaneous abortion is the most common complication of early pregnancy. In this study, we aim to investigate the clinical application value of genetic diagnosis using single nucleotide polymorphism (SNP) microarray analysis on the products of conception and to characterize the types of genetic abnormalities and their prevalence in pregnancy loss in Northwest China.Methods: Over 48 months, we selected 652 products of conception, which included chorionic villi, fetal tissues, germ cell samples, amniotic fluid samples, cord blood samples, and a cardiac blood sample. We analyzed the distribution of chromosomal abnormalities leading to fetal arrest or abortion using SNP array. The patients were then categorized divided into groups based on maternal age, gestational age, number of miscarriages, and maternal ethnic background. The incidences of various chromosomal abnormalities in each group were compared.Results: Of the 652 cases, 314 (48.16%) exhibited chromosomal abnormalities. These included 286 cases with numerical chromosomal abnormalities, 24 cases with copy number variation, and four cases with loss of heterozygosity. Among them, there were 203 trisomy cases, 55 monosomy cases, and 28 polyploidy cases. In the subgroup analysis, significant differences were found in the frequency of numerical chromosomal abnormalities and copy number variation between the advanced and younger maternal age group as well as between the early and late abortion groups. Furthermore, we identified significant differences in the frequency of numerical chromosomal abnormalities between the first spontaneous abortion and recurrent miscarriage groups. However, there were no significant differences in the frequency of numerical chromosomal abnormalities between the Han and Uighur groups.Conclusion: Our research highlights chromosomal abnormalities as the primary cause of spontaneous abortion, with a higher incidence in early pregnancy and among women of advanced age. The use of SNP array analysis emerges as an effective and reliable technique for chromosome analysis in aborted fetuses. This method offers a comprehensive and dependable genetic investigation into the etiology of miscarriage, establishing itself as a valuable routine selection for genetic analysis in cases of natural abortions. |
| first_indexed | 2024-03-08T23:09:56Z |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-08T23:09:56Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-e6045c03f28e4eb7b16fbf52cf92e5a92023-12-15T07:58:56ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-12-011410.3389/fgene.2023.13196241319624Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest ChinaShuYuan Xue0ShuYuan Xue1LiXia Wang2Jie Wei3YuTong Liu4GuiFeng Ding5PengGao Dai6The College of Life Sciences, Northwest University, Xi’an, Shanxi, ChinaPrenatal Diagnosis Center, Urumqi Maternal and Child Healthcare Hospital, Urumqi, Xinjiang, ChinaPrenatal Diagnosis Center, Urumqi Maternal and Child Healthcare Hospital, Urumqi, Xinjiang, ChinaPrenatal Diagnosis Center, Urumqi Maternal and Child Healthcare Hospital, Urumqi, Xinjiang, ChinaCollege of Public Health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Obstetrics, Urumqi Maternal and Child Healthcare Hospital, Urumqi, Xinjiang, ChinaThe College of Life Sciences, Northwest University, Xi’an, Shanxi, ChinaBackground: Spontaneous abortion is the most common complication of early pregnancy. In this study, we aim to investigate the clinical application value of genetic diagnosis using single nucleotide polymorphism (SNP) microarray analysis on the products of conception and to characterize the types of genetic abnormalities and their prevalence in pregnancy loss in Northwest China.Methods: Over 48 months, we selected 652 products of conception, which included chorionic villi, fetal tissues, germ cell samples, amniotic fluid samples, cord blood samples, and a cardiac blood sample. We analyzed the distribution of chromosomal abnormalities leading to fetal arrest or abortion using SNP array. The patients were then categorized divided into groups based on maternal age, gestational age, number of miscarriages, and maternal ethnic background. The incidences of various chromosomal abnormalities in each group were compared.Results: Of the 652 cases, 314 (48.16%) exhibited chromosomal abnormalities. These included 286 cases with numerical chromosomal abnormalities, 24 cases with copy number variation, and four cases with loss of heterozygosity. Among them, there were 203 trisomy cases, 55 monosomy cases, and 28 polyploidy cases. In the subgroup analysis, significant differences were found in the frequency of numerical chromosomal abnormalities and copy number variation between the advanced and younger maternal age group as well as between the early and late abortion groups. Furthermore, we identified significant differences in the frequency of numerical chromosomal abnormalities between the first spontaneous abortion and recurrent miscarriage groups. However, there were no significant differences in the frequency of numerical chromosomal abnormalities between the Han and Uighur groups.Conclusion: Our research highlights chromosomal abnormalities as the primary cause of spontaneous abortion, with a higher incidence in early pregnancy and among women of advanced age. The use of SNP array analysis emerges as an effective and reliable technique for chromosome analysis in aborted fetuses. This method offers a comprehensive and dependable genetic investigation into the etiology of miscarriage, establishing itself as a valuable routine selection for genetic analysis in cases of natural abortions.https://www.frontiersin.org/articles/10.3389/fgene.2023.1319624/fullsingle nucleotide polymorphism microarray analysischromosome aberrationscopy number variationpregnancy lossclinical application |
| spellingShingle | ShuYuan Xue ShuYuan Xue LiXia Wang Jie Wei YuTong Liu GuiFeng Ding PengGao Dai Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China Frontiers in Genetics single nucleotide polymorphism microarray analysis chromosome aberrations copy number variation pregnancy loss clinical application |
| title | Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China |
| title_full | Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China |
| title_fullStr | Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China |
| title_full_unstemmed | Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China |
| title_short | Clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in Northwest China |
| title_sort | clinical application of single nucleotide polymorphism microarray analysis in pregnancy loss in northwest china |
| topic | single nucleotide polymorphism microarray analysis chromosome aberrations copy number variation pregnancy loss clinical application |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1319624/full |
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